(Circular) (Six-base) MAPSORT of: pF1KE2552.seq Check: 5291 from: 1 to: 3614 pF1KE2552 3614 bp With 182 enzymes: SgfI * November 21, 2014 11:13 .. AccI GT'mk_AC Cuts at: 359 652 359 Size: 293 3321 Acc65I G'GTAC_C Cuts at: 630 630 Size: 3614 AclI AA'CG_TT Cuts at: 3454 3454 Size: 3614 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 279 397 1439 1871 2618 2886 279 Size: 118 1042 432 747 268 1007 Fragments arranged by size: 1042 1007 747 432 268 118 AflIII A'CryG_T Cuts at: 966 2070 3047 966 Size: 1104 977 1533 Fragments arranged by size: 1533 1104 977 AgeI A'CCGG_T Cuts at: 2002 2002 Size: 3614 AlwNI CAG_nnn'CTG Cuts at: 919 2486 919 Size: 1567 2047 ApaLI G'TGCA_C Cuts at: 2384 2384 Size: 3614 ApoI r'AATT_y Cuts at: 238 618 2967 3223 238 Size: 380 2349 256 629 Fragments arranged by size: 2349 629 380 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3614 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3614 AvaI C'yCGr_G Cuts at: 634 634 Size: 3614 BamHI G'GATC_C Cuts at: 639 639 Size: 3614 BanI G'GyrC_C Cuts at: 630 1044 1292 1327 630 Size: 414 248 35 2917 Fragments arranged by size: 2917 414 248 35 BanII G_rGCy'C Cuts at: 628 1658 628 Size: 1030 2584 BbeI G_GCGC'C Cuts at: 1048 1296 1048 Size: 248 3366 BbsI GAAGACnn'nnnn_ Cuts at: 309 309 Size: 3614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 133 167 133 Size: 34 3580 BciVI GTATCCnnnnn_n' Cuts at: 1512 2273 3614 1512 Size: 761 1341 1512 Fragments arranged by size: 1512 1341 761 BclI T'GATC_A Cuts at: 1134 1134 Size: 3614 BglII A'GATC_T Cuts at: 418 1129 3166 418 Size: 711 2037 866 Fragments arranged by size: 2037 866 711 BlpI GC'TnA_GC Cuts at: 726 726 Size: 3614 Bme1580I G_kGCm'C Cuts at: 1239 1332 2388 1239 Size: 93 1056 2465 Fragments arranged by size: 2465 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 826 907 1242 3386 826 Size: 81 335 2144 1054 Fragments arranged by size: 2144 1054 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1981 1981 Size: 3614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 368 368 Size: 3614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 790 1669 2161 2459 790 Size: 879 492 298 1945 Fragments arranged by size: 1945 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3318 3318 Size: 3614 BsaAI yAC'GTr Cuts at: 967 967 Size: 3614 BsaBI GATnn'nnATC Cuts at: 6 1128 3165 6 Size: 1122 2037 455 Fragments arranged by size: 2037 1122 455 BsaHI Gr'CG_yC Cuts at: 1045 1293 1995 3491 1045 Size: 248 702 1496 1168 Fragments arranged by size: 1496 1168 702 248 BsaWI w'CCGG_w Cuts at: 803 1324 2002 2276 2423 2834 803 Size: 521 678 274 147 411 1583 Fragments arranged by size: 1583 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 341 371 341 Size: 30 3584 BseYI C'CCAG_C Cuts at: 1039 1797 2374 2870 1039 Size: 758 577 496 1783 Fragments arranged by size: 1783 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1156 1202 2410 104 Size: 1052 46 1208 1308 Fragments arranged by size: 1308 1208 1052 46 BsiHKAI G_wGCw'C Cuts at: 628 1406 1596 2388 628 Size: 778 190 792 1854 Fragments arranged by size: 1854 792 778 190 Bsp1286I G_dGCh'C Cuts at: 628 1239 1332 1406 1596 1658 2388 628 Size: 611 93 74 190 62 730 1854 Fragments arranged by size: 1854 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2834 2834 Size: 3614 BspHI T'CATG_A Cuts at: 2790 2790 Size: 3614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 664 878 1180 1561 664 Size: 214 302 381 2717 Fragments arranged by size: 2717 381 302 214 BsrBI CCG'CTC Cuts at: 50 1906 1960 3444 3609 50 Size: 1856 54 1484 165 55 Fragments arranged by size: 1856 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 347 1526 347 Size: 1179 2435 BsrFI r'CCGG_y Cuts at: 1612 1793 2002 1612 Size: 181 209 3224 Fragments arranged by size: 3224 209 181 BssSI C'ACGA_G Cuts at: 1885 2243 1885 Size: 358 3256 BstAPI GCAn_nnn'nTGC Cuts at: 991 991 Size: 3614 BstBI TT'CG_AA Cuts at: 1975 1975 Size: 3614 BstXI CCAn_nnnn'nTGG Cuts at: 222 222 Size: 3614 BstYI r'GATC_y Cuts at: 1 418 639 1106 1129 1464 1710 2722 2808 Size: 417 221 467 23 335 246 1012 86 Cuts at: 2808 2820 3166 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 417 346 335 246 221 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3614 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1564 1745 1908 1564 Size: 181 163 3270 Fragments arranged by size: 3270 181 163 BtsI GCAGTG_nn' Cuts at: 857 857 Size: 3614 DraI TTT'AAA Cuts at: 613 613 Size: 3614 DrdI GACnn_nn'nnGTC Cuts at: 1320 2178 1320 Size: 858 2756 EaeI y'GGCC_r Cuts at: 1199 1373 1764 1791 3136 1199 Size: 174 391 27 1345 1677 Fragments arranged by size: 1677 1345 391 174 27 EagI C'GGCC_G Cuts at: 1199 1199 Size: 3614 EarI CTCTTCn'nnn_ Cuts at: 1637 1847 1637 Size: 210 3404 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1822 2132 2278 3424 1822 Size: 310 146 1146 2012 Fragments arranged by size: 2012 1146 310 146 EcoICRI GAG'CTC Cuts at: 626 626 Size: 3614 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 279 368 397 1439 1871 2618 2886 279 Size: 89 29 1042 432 747 268 1007 Fragments arranged by size: 1042 1007 747 432 268 89 29 EcoO109I rG'GnC_Cy Cuts at: 753 753 Size: 3614 EcoRI G'AATT_C Cuts at: 618 618 Size: 3614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1633 1633 Size: 3614 FspI TGC'GCA Cuts at: 1395 1395 Size: 3614 HaeII r_GCGC'y Cuts at: 1048 1296 2318 3297 1048 Size: 248 1022 979 1365 Fragments arranged by size: 1365 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 341 373 1456 1486 1488 1518 341 Size: 32 1083 30 2 30 2437 Fragments arranged by size: 2437 1083 32 30 30 2 HincII GTy'rAC Cuts at: 653 653 Size: 3614 KasI G'GCGC_C Cuts at: 1044 1292 1044 Size: 248 3366 KpnI G_GTAC'C Cuts at: 634 634 Size: 3614 MluI A'CGCG_T Cuts at: 3047 3047 Size: 3614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2285 2469 2285 Size: 184 3430 MscI TGG'CCA Cuts at: 1375 1375 Size: 3614 MslI CAynn'nnrTG Cuts at: 1603 1730 1603 Size: 127 3487 MspA1I CmG'CkG Cuts at: 725 1039 1399 2412 2657 725 Size: 314 360 1013 245 1682 Fragments arranged by size: 1682 1013 360 314 245 NaeI GCC'GGC Cuts at: 1795 1795 Size: 3614 NarI GG'CG_CC Cuts at: 1045 1293 1045 Size: 248 3366 NcoI C'CATG_G Cuts at: 107 107 Size: 3614 NgoMIV G'CCGG_C Cuts at: 1793 1793 Size: 3614 NspI r_CATG'y Cuts at: 667 1167 2074 667 Size: 500 907 2207 Fragments arranged by size: 2207 907 500 PciI A'CATG_T Cuts at: 2070 2070 Size: 3614 PflMI CCAn_nnn'nTGG Cuts at: 470 470 Size: 3614 PmeI GTTT'AAAC Cuts at: 613 613 Size: 3614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2753 2785 2753 Size: 32 3582 PstI C_TGCA'G Cuts at: 661 1346 661 Size: 685 2929 PvuI CG_AT'CG Cuts at: 104 104 Size: 3614 PvuII CAG'CTG Cuts at: 1039 1399 1039 Size: 360 3254 RsrII CG'GwC_CG Cuts at: 1809 1809 Size: 3614 SacI G_AGCT'C Cuts at: 628 628 Size: 3614 SalI G'TCGA_C Cuts at: 651 651 Size: 3614 SapI GCTCTTCn'nnn_ Cuts at: 1637 1847 1637 Size: 210 3404 SbfI CC_TGCA'GG Cuts at: 661 661 Size: 3614 ScaI AGT'ACT Cuts at: 3131 3131 Size: 3614 SfcI C'TryA_G Cuts at: 33 657 1342 2335 2526 33 Size: 624 685 993 191 1121 Fragments arranged by size: 1121 993 685 624 191 SfoI GGC'GCC Cuts at: 1046 1294 1046 Size: 248 3366 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3614 SmaI CCC'GGG Cuts at: 636 636 Size: 3614 SmlI C'TyrA_G Cuts at: 769 1684 2176 2438 769 Size: 915 492 262 1945 Fragments arranged by size: 1945 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3614 SphI G_CATG'C Cuts at: 667 1167 667 Size: 500 3114 StyI C'CwwG_G Cuts at: 107 748 1102 107 Size: 641 354 2619 Fragments arranged by size: 2619 641 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 232 1197 1330 1789 1998 232 Size: 965 133 459 209 1848 Fragments arranged by size: 1848 965 459 209 133 TatI w'GTAC_w Cuts at: 3129 3129 Size: 3614 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3131 3163 3131 Size: 32 3582 Tth111I GACn'n_nGTC Cuts at: 1411 1411 Size: 3614 XbaI T'CTAG_A Cuts at: 645 645 Size: 3614 XcmI CCAnnnn_n'nnnnTGG Cuts at: 219 1109 219 Size: 890 2724 XmaI C'CCGG_G Cuts at: 634 634 Size: 3614 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI