################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI Hin1I KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 68 CGCG CG^CG AclI 373 AACGTT AA^CGTT AflIII 257 ACATGT A^C[GA][CT]GT AhdI 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 820 ATTAAT AT^TAAT AsnI 820 ATTAAT AT^TAAT AspEI 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2514 GAGCTC G[AT]GC[AT]^C BanI 2298 GGTGCC G^G[CT][GA]CC BglII 811 AGATCT A^GATCT BsaBI 2524 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1005 GTGCAG GTGCAG(16/14) BsiEI 1074 CGGTCG CG[GA][CT]^CG BsiHKAI 2514 GAGCTC G[AT]GC[AT]^C BspLU11I 257 ACATGT A^CATGT BsrFI 197 ACCGGC [GA]^CCGG[CT] BstAPI 764 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 68 CGCG CG^CG BstXI 1352 CCATTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 197 ACCGGC [GA]^CCGG[CT] DraI 1095 TTTAAA TTT^AAA DsaI 2762 CCATGG C^C[GA][CT]GG Eam1105I 1364 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2014 GAAGAG CTCTTC(1/4) EcoNI 714 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1872 GATATC GAT^ATC HaeII 49 GGCGCT [GA]GCGC^[CT] HincII 376 GTTAAC GT[CT]^[GA]AC HindII 376 GTTAAC GT[CT]^[GA]AC HpaI 376 GTTAAC GTT^AAC Ksp632I 2014 GAAGAG CTCTTC(1/4) MamI 2524 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1799 CAATTG C^AATTG MunI 1799 CAATTG C^AATTG MvnI 68 CGCG CG^CG NcoI 2762 CCATGG C^CATGG PaeR7I 476 CTCGAG C^TCGAG PflMI 2240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1757 GGGACCC [GA]G^G[AT]CC[CT] PshBI 820 ATTAAT AT^TAAT Psp1406I 373 AACGTT AA^CGTT SacI 2514 GAGCTC GAGCT^C SgrAI 196 CACCGGCG C[GA]^CCGG[CT]G SmaI 44 CCCGGG CCC^GGG SphI 1647 GCATGC GCATG^C StuI 834 AGGCCT AGG^CCT Tsp45I 658 GTGAC ^GT[GC]AC Van91I 2240 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 425 CCAATGAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 476 CTCGAG C^TCGAG XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 2284 GTAC GT^AC 2599 GTAC ApaI 22 GGGCCC GGGCC^C 1548 GGGCCC AvaI 44 CCCGGG C^[CT]CG[GA]G 476 CTCGAG BclI 1068 TGATCA T^GATCA 2177 TGATCA BseRI 292 GAGGAG GAGGAG(10/8) 2263 GAGGAG BsmAI 1718 GAGAC GTCTC(1/5) 2678 GTCTC BsoBI 44 CCCGGG C^[CT]CG[GA]G 476 CTCGAG EcoT14I 1553 CCAAGG C^C[AT][AT]GG 2762 CCATGG FauI 129 CCCGC CCCGC(4/6) 138 CCCGC FbaI 1068 TGATCA T^GATCA 2177 TGATCA HgaI 1675 GACGC GACGC(5/10) 2209 GACGC HindIII 1854 AAGCTT A^AGCTT 2335 AAGCTT MaeII 374 ACGT A^CGT 2114 ACGT MslI 330 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1923 CATTATGGTG PstI 1867 CTGCAG CTGCA^G 2602 CTGCAG RsaI 2284 GTAC GT^AC 2599 GTAC SspI 925 AATATT AAT^ATT 1559 AATATT StyI 1553 CCAAGG C^C[AT][AT]GG 2762 CCATGG TfiI 1179 GAATC G^A[AT]TC 1631 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RcaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI