(Linear) (Six-base) MAPSORT of: KIEE1965.seq Check: 3360 from: 1 to: 1587 KIEE1965.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1544 1587 Size: 926 618 43 Fragments arranged by size: 926 618 43 AhdI GACnn_n'nnGTC Cuts at: 0 231 1587 Size: 231 1356 AleI CACnn'nnGTG Cuts at: 0 217 1587 Size: 217 1370 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 647 679 1587 Size: 647 32 908 Fragments arranged by size: 908 647 32 AlwNI CAG_nnn'CTG Cuts at: 0 672 1587 Size: 672 915 ApoI r'AATT_y Cuts at: 0 397 955 1587 Size: 397 558 632 Fragments arranged by size: 632 558 397 AvaI C'yCGr_G Cuts at: 0 877 1587 Size: 877 710 BanI G'GyrC_C Cuts at: 0 1208 1587 Size: 1208 379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 361 395 1587 Size: 361 34 1192 Fragments arranged by size: 1192 361 34 BfrBI ATG'CAT Cuts at: 0 537 1171 1587 Size: 537 634 416 Fragments arranged by size: 634 537 416 BglI GCCn_nnn'nGGC Cuts at: 0 1474 1587 Size: 1474 113 BglII A'GATC_T Cuts at: 0 1329 1587 Size: 1329 258 Bme1580I G_kGCm'C Cuts at: 0 384 1587 Size: 384 1203 BmrI ACTGGGnnnn_n' Cuts at: 0 1064 1587 Size: 1064 523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 603 1587 Size: 603 984 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 120 150 1587 Size: 120 30 1437 Fragments arranged by size: 1437 120 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 66 798 853 1531 1587 Size: 66 732 55 678 56 Fragments arranged by size: 732 678 66 56 55 BseYI C'CCAG_C Cuts at: 0 792 1587 Size: 792 795 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1587 Size: 318 1269 BsiHKAI G_wGCw'C Cuts at: 0 274 1587 Size: 274 1313 BsmI GAATG_Cn' Cuts at: 0 106 1587 Size: 106 1481 BsmBI CGTCTCn'nnnn_ Cuts at: 0 336 1587 Size: 336 1251 Bsp1286I G_dGCh'C Cuts at: 0 274 384 1587 Size: 274 110 1203 Fragments arranged by size: 1203 274 110 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 271 1587 Size: 271 1316 BsrBI CCG'CTC Cuts at: 0 119 1587 Size: 119 1468 BsrDI GCAATG_nn' Cuts at: 0 873 1587 Size: 873 714 BsrGI T'GTAC_A Cuts at: 0 1295 1587 Size: 1295 292 BstXI CCAn_nnnn'nTGG Cuts at: 0 274 1332 1587 Size: 274 1058 255 Fragments arranged by size: 1058 274 255 BstYI r'GATC_y Cuts at: 0 571 1135 1329 1418 1587 Size: 571 564 194 89 169 Fragments arranged by size: 571 564 194 169 89 BtgI C'CryG_G Cuts at: 0 112 992 1587 Size: 112 880 595 Fragments arranged by size: 880 595 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1459 1587 Size: 1459 128 BtsI GCAGTG_nn' Cuts at: 0 559 1201 1463 1587 Size: 559 642 262 124 Fragments arranged by size: 642 559 262 124 DrdI GACnn_nn'nnGTC Cuts at: 0 526 1587 Size: 526 1061 EaeI y'GGCC_r Cuts at: 0 51 138 468 1587 Size: 51 87 330 1119 Fragments arranged by size: 1119 330 87 51 EarI CTCTTCn'nnn_ Cuts at: 0 643 945 1223 1587 Size: 643 302 278 364 Fragments arranged by size: 643 364 302 278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 603 926 1544 1587 Size: 603 323 618 43 Fragments arranged by size: 618 603 323 43 EcoO109I rG'GnC_Cy Cuts at: 0 260 461 883 1587 Size: 260 201 422 704 Fragments arranged by size: 704 422 260 201 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 333 1587 Size: 333 1254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 56 88 217 249 563 595 1357 1389 Size: 56 32 129 32 314 32 762 32 Cuts at: 1389 1587 Size: 198 Fragments arranged by size: 762 314 198 129 56 32 32 32 32 HincII GTy'rAC Cuts at: 0 1179 1587 Size: 1179 408 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1396 1587 Size: 1396 191 MscI TGG'CCA Cuts at: 0 53 140 470 1587 Size: 53 87 330 1117 Fragments arranged by size: 1117 330 87 53 MslI CAynn'nnrTG Cuts at: 0 217 542 1105 1587 Size: 217 325 563 482 Fragments arranged by size: 563 482 325 217 MspA1I CmG'CkG Cuts at: 0 49 946 1587 Size: 49 897 641 Fragments arranged by size: 897 641 49 NcoI C'CATG_G Cuts at: 0 992 1587 Size: 992 595 NsiI A_TGCA'T Cuts at: 0 539 1173 1587 Size: 539 634 414 Fragments arranged by size: 634 539 414 NspI r_CATG'y Cuts at: 0 1086 1587 Size: 1086 501 PflMI CCAn_nnn'nTGG Cuts at: 0 1054 1587 Size: 1054 533 PfoI T'CCnGG_A Cuts at: 0 900 1587 Size: 900 687 PpuMI rG'GwC_Cy Cuts at: 0 260 461 883 1587 Size: 260 201 422 704 Fragments arranged by size: 704 422 260 201 PshAI GACnn'nnGTC Cuts at: 0 128 1587 Size: 128 1459 PsiI TTA'TAA Cuts at: 0 254 1587 Size: 254 1333 PvuII CAG'CTG Cuts at: 0 946 1587 Size: 946 641 SfcI C'TryA_G Cuts at: 0 825 1344 1587 Size: 825 519 243 Fragments arranged by size: 825 519 243 SphI G_CATG'C Cuts at: 0 1086 1587 Size: 1086 501 SspI AAT'ATT Cuts at: 0 513 1587 Size: 513 1074 StyI C'CwwG_G Cuts at: 0 625 928 992 1434 1587 Size: 625 303 64 442 153 Fragments arranged by size: 625 442 303 153 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 507 1263 1587 Size: 507 756 324 Fragments arranged by size: 756 507 324 TatI w'GTAC_w Cuts at: 0 17 749 842 1295 1587 Size: 17 732 93 453 292 Fragments arranged by size: 732 453 292 93 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 120 152 1587 Size: 120 32 1435 Fragments arranged by size: 1435 120 32 Tth111I GACn'n_nGTC Cuts at: 0 445 1587 Size: 445 1142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 46 478 707 982 1587 Size: 46 432 229 275 605 Fragments arranged by size: 605 432 275 229 46 Enzymes that do cut: AcuI AhdI AleI AloI AlwNI ApoI AvaI BanI BcgI BfrBI BglI BglII Bme1580I BmrI BpmI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI PpuMI PshAI PsiI PvuII SfcI SphI SspI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BciVI BclI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI XbaI XhoI XmaI XmnI ZraI