################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BciVI BclI BfaI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MamI MluI MroI MseI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AvaI 181 CCCGAG C^[CT]CG[GA]G AviII 290 TGCGCA TGC^GCA BamHI 155 GGATCC G^GATCC BbrPI 1135 CACGTG CAC^GTG BbvCI 489 CCTCAGC CCTCAGC(-5/-2) BglII 866 AGATCT A^GATCT BlpI 715 GCTGAGC GC^T[ACGT]AGC Bpu1102I 715 GCTGAGC GC^T[ACGT]AGC BsaAI 1135 CACGTG [CT]AC^GT[GA] BsgI 422 GTGCAG GTGCAG(16/14) BsmI 293 GCATTC GAATGC(1/-1) BsoBI 181 CCCGAG C^[CT]CG[GA]G BspHI 1298 TCATGA T^CATGA BspMI 1260 GCAGGT ACCTGC(4/8) BssSI 1273 CACGAG CACGAG(-5/-1) BstAPI 1260 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 942 GGTGACC G^GT[ACGT]ACC BstPI 942 GGTGACC G^GT[ACGT]ACC CelII 715 GCTGAGC GC^T[ACGT]AGC DraIII 1239 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 213 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 942 GGTGACC G^GT[ACGT]ACC FspI 290 TGCGCA TGC^GCA KspI 19 CCGCGG CCGC^GG MaeII 1136 ACGT A^CGT MfeI 204 CAATTG C^AATTG MunI 204 CAATTG C^AATTG PflMI 498 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 972 GAGTC GAGTC(4/5) PmaCI 1135 CACGTG CAC^GTG PmlI 1135 CACGTG CAC^GTG RcaI 1298 TCATGA T^CATGA SacI 718 GAGCTC GAGCT^C SacII 19 CCGCGG CCGC^GG SapI 240 GCTCTTC GCTCTTC(1/4) Tsp509I 205 AATT ^AATT Van91I 498 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Aor51HI 342 AGCGCT AGC^GCT 879 AGCGCT Asp700I 87 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 836 GAAAACCTTC AspHI 162 GAGCAC G[AT]GC[AT]^C 718 GAGCTC BbeI 1251 GGCGCC GGCGC^C 1291 GGCGCC BcgI 564 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 437 GCAGCCTCTTCG BsaI 565 GAGACC GGTCTC(1/5) 706 GAGACC BsiHKAI 162 GAGCAC G[AT]GC[AT]^C 718 GAGCTC BsmBI 11 GAGACG CGTCTC(1/5) 283 GAGACG Eco47III 342 AGCGCT AGC^GCT 879 AGCGCT EcoNI 789 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1350 CCTCTTCAAGG KasI 1251 GGCGCC G^GCGCC 1291 GGCGCC NarI 1251 GGCGCC GG^CGCC 1291 GGCGCC PpuMI 177 GGGTCCC [GA]G^G[AT]CC[CT] 1147 AGGTCCC PvuII 226 CAGCTG CAG^CTG 451 CAGCTG SexAI 35 ACCTGGT A^CC[AT]GGT 1226 ACCTGGT SfcI 430 CTACAG C^T[GA][CT]AG 474 CTACAG SfoI 1251 GGCGCC GGC^GCC 1291 GGCGCC TfiI 744 GATTC G^A[AT]TC 805 GAATC XcmI 498 CCAGGGCCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 929 CCATCACAGCCATGG XmnI 87 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 836 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XhoII