(Circular) (Six-base) MAPSORT of: pF1KE3532.seq Check: 7790 from: 1 to: 4364 pF1KE3532 4364 bp With 182 enzymes: SgfI * August 29, 2012 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 174 174 Size: 4364 AccI GT'mk_AC Cuts at: 1402 1402 Size: 4364 Acc65I G'GTAC_C Cuts at: 1380 1380 Size: 4364 AclI AA'CG_TT Cuts at: 4204 4204 Size: 4364 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 2621 3368 3636 2189 Size: 432 747 268 2917 Fragments arranged by size: 2917 747 432 268 AflIII A'CryG_T Cuts at: 1716 2820 3797 1716 Size: 1104 977 2283 Fragments arranged by size: 2283 1104 977 AgeI A'CCGG_T Cuts at: 2752 2752 Size: 4364 AleI CACnn'nnGTG Cuts at: 1187 1187 Size: 4364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1067 1067 Size: 4364 AlwNI CAG_nnn'CTG Cuts at: 1017 1669 3236 1017 Size: 652 1567 2145 Fragments arranged by size: 2145 1567 652 ApaI G_GGCC'C Cuts at: 383 618 383 Size: 235 4129 ApaLI G'TGCA_C Cuts at: 997 3134 997 Size: 2137 2227 ApoI r'AATT_y Cuts at: 1368 3717 3973 1368 Size: 2349 256 1759 Fragments arranged by size: 2349 1759 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4364 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4364 AvaI C'yCGr_G Cuts at: 1384 1384 Size: 4364 AvrII C'CTAG_G Cuts at: 1240 1240 Size: 4364 BamHI G'GATC_C Cuts at: 1281 1389 1281 Size: 108 4256 BanI G'GyrC_C Cuts at: 132 150 596 708 775 1139 1380 1794 2042 Size: 18 446 112 67 364 241 414 248 Cuts at: 2042 2077 132 Size: 35 2419 Fragments arranged by size: 2419 446 414 364 248 241 112 67 35 18 BanII G_rGCy'C Cuts at: 383 618 1378 2408 383 Size: 235 760 1030 2339 Fragments arranged by size: 2339 1030 760 235 BbeI G_GCGC'C Cuts at: 600 712 779 1798 2046 600 Size: 112 67 1019 248 2918 Fragments arranged by size: 2918 1019 248 112 67 BciVI GTATCCnnnnn_n' Cuts at: 2262 3023 4364 2262 Size: 761 1341 2262 Fragments arranged by size: 2262 1341 761 BclI T'GATC_A Cuts at: 1884 1884 Size: 4364 BglI GCCn_nnn'nGGC Cuts at: 393 706 802 393 Size: 313 96 3955 Fragments arranged by size: 3955 313 96 BglII A'GATC_T Cuts at: 1879 3916 1879 Size: 2037 2327 BlpI GC'TnA_GC Cuts at: 1476 1476 Size: 4364 Bme1580I G_kGCm'C Cuts at: 266 383 606 618 1001 1088 1989 2082 3138 Size: 117 223 12 383 87 901 93 1056 Cuts at: 3138 266 Size: 1492 Fragments arranged by size: 1492 1056 901 383 223 117 93 87 12 BmgBI CAC'GTC Cuts at: 1189 1189 Size: 4364 BmrI ACTGGGnnnn_n' Cuts at: 1220 1576 1657 1992 4136 1220 Size: 356 81 335 2144 1448 Fragments arranged by size: 2144 1448 356 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 532 1122 2731 532 Size: 590 1609 2165 Fragments arranged by size: 2165 1609 590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 751 751 Size: 4364 Bpu10I CC'TnA_GC Cuts at: 1018 1018 Size: 4364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1540 2419 2911 3209 1540 Size: 879 492 298 2695 Fragments arranged by size: 2695 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4068 4068 Size: 4364 BsaAI yAC'GTr Cuts at: 1717 1717 Size: 4364 BsaBI GATnn'nnATC Cuts at: 6 1878 3915 6 Size: 1872 2037 455 Fragments arranged by size: 2037 1872 455 BsaHI Gr'CG_yC Cuts at: 597 709 776 1795 2043 2745 4241 597 Size: 112 67 1019 248 702 1496 720 Fragments arranged by size: 1496 1019 720 702 248 112 67 BsaWI w'CCGG_w Cuts at: 1553 2074 2752 3026 3173 3584 1553 Size: 521 678 274 147 411 2333 Fragments arranged by size: 2333 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1297 1297 Size: 4364 BseYI C'CCAG_C Cuts at: 236 1081 1789 2547 3124 3620 236 Size: 845 708 758 577 496 980 Fragments arranged by size: 980 845 758 708 577 496 BsiEI CG_ry'CG Cuts at: 104 496 764 1906 1952 3160 104 Size: 392 268 1142 46 1208 1308 Fragments arranged by size: 1308 1208 1142 392 268 46 BsiHKAI G_wGCw'C Cuts at: 587 1001 1024 1118 1135 1378 2156 2346 3138 Size: 414 23 94 17 243 778 190 792 Cuts at: 3138 587 Size: 1813 Fragments arranged by size: 1813 792 778 414 243 190 94 23 17 BsmBI CGTCTCn'nnnn_ Cuts at: 277 277 Size: 4364 Bsp1286I G_dGCh'C Cuts at: 266 383 587 606 618 1001 1024 1088 1118 Size: 117 204 19 12 383 23 64 30 Cuts at: 1118 1135 1378 1989 2082 2156 2346 2408 3138 Size: 17 243 611 93 74 190 62 730 Cuts at: 3138 266 Size: 1492 Fragments arranged by size: 1492 730 611 383 243 204 190 117 93 74 64 62 30 23 19 17 12 BspEI T'CCGG_A Cuts at: 3584 3584 Size: 4364 BspHI T'CATG_A Cuts at: 3540 3540 Size: 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 174 1035 1414 1628 1930 2311 174 Size: 861 379 214 302 381 2227 Fragments arranged by size: 2227 861 381 379 302 214 BsrBI CCG'CTC Cuts at: 50 2656 2710 4194 4359 50 Size: 2606 54 1484 165 55 Fragments arranged by size: 2606 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2276 2276 Size: 4364 BsrFI r'CCGG_y Cuts at: 845 1001 1314 2362 2543 2752 845 Size: 156 313 1048 181 209 2457 Fragments arranged by size: 2457 1048 313 209 181 156 BssHII G'CGCG_C Cuts at: 665 665 Size: 4364 BssSI C'ACGA_G Cuts at: 2635 2993 2635 Size: 358 4006 BstAPI GCAn_nnn'nTGC Cuts at: 1139 1741 1139 Size: 602 3762 BstBI TT'CG_AA Cuts at: 2725 2725 Size: 4364 BstXI CCAn_nnnn'nTGG Cuts at: 375 978 1285 375 Size: 603 307 3454 Fragments arranged by size: 3454 603 307 BstYI r'GATC_y Cuts at: 1 1281 1389 1856 1879 2214 2460 3472 3558 Size: 1280 108 467 23 335 246 1012 86 Cuts at: 3558 3570 3916 1 Size: 12 346 449 Fragments arranged by size: 1280 1012 467 449 346 335 246 108 86 23 12 BtgI C'CryG_G Cuts at: 107 779 107 Size: 672 3692 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 781 2314 2495 2658 781 Size: 1533 181 163 2487 Fragments arranged by size: 2487 1533 181 163 BtsI GCAGTG_nn' Cuts at: 1607 1607 Size: 4364 DraI TTT'AAA Cuts at: 1363 1363 Size: 4364 DraIII CAC_nnn'GTG Cuts at: 1139 1139 Size: 4364 DrdI GACnn_nn'nnGTC Cuts at: 2070 2928 2070 Size: 858 3506 EaeI y'GGCC_r Cuts at: 493 761 1154 1312 1949 2123 2514 2541 3886 Size: 268 393 158 637 174 391 27 1345 Cuts at: 3886 493 Size: 971 Fragments arranged by size: 1345 971 637 393 391 268 174 158 27 EagI C'GGCC_G Cuts at: 493 761 1949 493 Size: 268 1188 2908 Fragments arranged by size: 2908 1188 268 EarI CTCTTCn'nnn_ Cuts at: 223 340 952 2387 2597 223 Size: 117 612 1435 210 1990 Fragments arranged by size: 1990 1435 612 210 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 907 2572 2882 3028 4174 907 Size: 1665 310 146 1146 1097 Fragments arranged by size: 1665 1146 1097 310 146 EcoICRI GAG'CTC Cuts at: 1376 1376 Size: 4364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 751 2189 2621 3368 3636 751 Size: 1438 432 747 268 1479 Fragments arranged by size: 1479 1438 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 379 380 530 542 615 1014 1222 1244 1503 Size: 1 150 12 73 399 208 22 259 Cuts at: 1503 379 Size: 3240 Fragments arranged by size: 3240 399 259 208 150 73 22 12 1 EcoRI G'AATT_C Cuts at: 1368 1368 Size: 4364 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 4364 FspI TGC'GCA Cuts at: 2145 2145 Size: 4364 HaeII r_GCGC'y Cuts at: 600 712 779 1798 2046 3068 4047 600 Size: 112 67 1019 248 1022 979 917 Fragments arranged by size: 1022 1019 979 917 248 112 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2206 2236 2238 2268 2206 Size: 30 2 30 4302 Fragments arranged by size: 4302 30 30 2 HincII GTy'rAC Cuts at: 1403 1403 Size: 4364 KasI G'GCGC_C Cuts at: 596 708 775 1794 2042 596 Size: 112 67 1019 248 2918 Fragments arranged by size: 2918 1019 248 112 67 KpnI G_GTAC'C Cuts at: 1384 1384 Size: 4364 MluI A'CGCG_T Cuts at: 3797 3797 Size: 4364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3035 3219 3035 Size: 184 4180 MscI TGG'CCA Cuts at: 2125 2125 Size: 4364 MslI CAynn'nnrTG Cuts at: 1187 2353 2480 1187 Size: 1166 127 3071 Fragments arranged by size: 3071 1166 127 MspA1I CmG'CkG Cuts at: 190 1475 1789 2149 3162 3407 190 Size: 1285 314 360 1013 245 1147 Fragments arranged by size: 1285 1147 1013 360 314 245 NaeI GCC'GGC Cuts at: 847 1316 2545 847 Size: 469 1229 2666 Fragments arranged by size: 2666 1229 469 NarI GG'CG_CC Cuts at: 597 709 776 1795 2043 597 Size: 112 67 1019 248 2918 Fragments arranged by size: 2918 1019 248 112 67 NcoI C'CATG_G Cuts at: 107 779 107 Size: 672 3692 NgoMIV G'CCGG_C Cuts at: 845 1314 2543 845 Size: 469 1229 2666 Fragments arranged by size: 2666 1229 469 NotI GC'GGCC_GC Cuts at: 761 761 Size: 4364 NspI r_CATG'y Cuts at: 1417 1917 2824 1417 Size: 500 907 2957 Fragments arranged by size: 2957 907 500 PasI CC'CwG_GG Cuts at: 177 611 177 Size: 434 3930 PciI A'CATG_T Cuts at: 2820 2820 Size: 4364 PmeI GTTT'AAAC Cuts at: 1363 1363 Size: 4364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 3503 Size: 32 4332 PpuMI rG'GwC_Cy Cuts at: 1014 1014 Size: 4364 PspOMI G'GGCC_C Cuts at: 379 614 379 Size: 235 4129 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 907 939 907 Size: 32 4332 PstI C_TGCA'G Cuts at: 425 1411 2096 425 Size: 986 685 2693 Fragments arranged by size: 2693 986 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4364 PvuII CAG'CTG Cuts at: 190 1789 2149 190 Size: 1599 360 2405 Fragments arranged by size: 2405 1599 360 RsrII CG'GwC_CG Cuts at: 2559 2559 Size: 4364 SacI G_AGCT'C Cuts at: 1378 1378 Size: 4364 SalI G'TCGA_C Cuts at: 1401 1401 Size: 4364 SapI GCTCTTCn'nnn_ Cuts at: 223 2387 2597 223 Size: 2164 210 1990 Fragments arranged by size: 2164 1990 210 SbfI CC_TGCA'GG Cuts at: 1411 1411 Size: 4364 ScaI AGT'ACT Cuts at: 3881 3881 Size: 4364 SexAI A'CCwGG_T Cuts at: 896 926 1135 896 Size: 30 209 4125 Fragments arranged by size: 4125 209 30 SfcI C'TryA_G Cuts at: 33 421 1407 2092 3085 3276 33 Size: 388 986 685 993 191 1121 Fragments arranged by size: 1121 993 986 685 388 191 SfoI GGC'GCC Cuts at: 598 710 777 1796 2044 598 Size: 112 67 1019 248 2918 Fragments arranged by size: 2918 1019 248 112 67 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4364 SmaI CCC'GGG Cuts at: 1386 1386 Size: 4364 SmlI C'TyrA_G Cuts at: 1519 2434 2926 3188 1519 Size: 915 492 262 2695 Fragments arranged by size: 2695 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4364 SphI G_CATG'C Cuts at: 1417 1917 1417 Size: 500 3864 StyI C'CwwG_G Cuts at: 107 479 737 779 830 1240 1498 1852 107 Size: 372 258 42 51 410 258 354 2619 Fragments arranged by size: 2619 410 372 354 258 258 51 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 760 1049 1947 2080 2539 2748 760 Size: 289 898 133 459 209 2376 Fragments arranged by size: 2376 898 459 289 209 133 TatI w'GTAC_w Cuts at: 3879 3879 Size: 4364 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3881 3913 3881 Size: 32 4332 Tth111I GACn'n_nGTC Cuts at: 2161 2161 Size: 4364 XbaI T'CTAG_A Cuts at: 1395 1395 Size: 4364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 975 978 1036 1859 975 Size: 3 58 823 3480 Fragments arranged by size: 3480 823 58 3 XmaI C'CCGG_G Cuts at: 1384 1384 Size: 4364 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AloI AscI BaeI BbsI BbvCI BcgI BfrBI BmtI BsaXI BsgI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI