Restriction map for
pF1KE3532
(Linear) (Six-base) MAPSORT of: KIEE3532.seq Check: 5580 from: 1 to: 1251
KIEE3532.orf
With 182 enzymes: SgfI *
August 29, 2012 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 66 1251
Size: 66 1185
AleI CACnn'nnGTG
Cuts at: 0 1079 1251
Size: 1079 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 959 1251
Size: 959 292
AlwNI CAG_nnn'CTG
Cuts at: 0 909 1251
Size: 909 342
ApaI G_GGCC'C
Cuts at: 0 275 510 1251
Size: 275 235 741
Fragments arranged by size:
741 275 235
ApaLI G'TGCA_C
Cuts at: 0 889 1251
Size: 889 362
AvrII C'CTAG_G
Cuts at: 0 1132 1251
Size: 1132 119
BamHI G'GATC_C
Cuts at: 0 1173 1251
Size: 1173 78
BanI G'GyrC_C
Cuts at: 0 24 42 488 600 667 1031 1251
Size: 24 18 446 112 67 364 220
Fragments arranged by size:
446 364 220 112 67 24 18
BanII G_rGCy'C
Cuts at: 0 275 510 1251
Size: 275 235 741
Fragments arranged by size:
741 275 235
BbeI G_GCGC'C
Cuts at: 0 492 604 671 1251
Size: 492 112 67 580
Fragments arranged by size:
580 492 112 67
BglI GCCn_nnn'nGGC
Cuts at: 0 285 598 694 1251
Size: 285 313 96 557
Fragments arranged by size:
557 313 285 96
Bme1580I G_kGCm'C
Cuts at: 0 158 275 498 510 893 980 1251
Size: 158 117 223 12 383 87 271
Fragments arranged by size:
383 271 223 158 117 87 12
BmgBI CAC'GTC
Cuts at: 0 1081 1251
Size: 1081 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 1112 1251
Size: 1112 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 424 1014 1251
Size: 424 590 237
Fragments arranged by size:
590 424 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1251
Size: 643 608
Bpu10I CC'TnA_GC
Cuts at: 0 910 1251
Size: 910 341
BsaHI Gr'CG_yC
Cuts at: 0 489 601 668 1251
Size: 489 112 67 583
Fragments arranged by size:
583 489 112 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1189 1251
Size: 1189 62
BseYI C'CCAG_C
Cuts at: 0 128 973 1251
Size: 128 845 278
Fragments arranged by size:
845 278 128
BsiEI CG_ry'CG
Cuts at: 0 388 656 1251
Size: 388 268 595
Fragments arranged by size:
595 388 268
BsiHKAI G_wGCw'C
Cuts at: 0 479 893 916 1010 1027 1251
Size: 479 414 23 94 17 224
Fragments arranged by size:
479 414 224 94 23 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 1251
Size: 169 1082
Bsp1286I G_dGCh'C
Cuts at: 0 158 275 479 498 510 893 916 980
Size: 158 117 204 19 12 383 23 64
Cuts at: 980 1010 1027 1251
Size: 30 17 224
Fragments arranged by size:
383 224 204 158 117 64 30 23
19 17 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 927 1251
Size: 66 861 324
Fragments arranged by size:
861 324 66
BsrFI r'CCGG_y
Cuts at: 0 737 893 1206 1251
Size: 737 156 313 45
Fragments arranged by size:
737 313 156 45
BssHII G'CGCG_C
Cuts at: 0 557 1251
Size: 557 694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1031 1251
Size: 1031 220
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 870 1177 1251
Size: 267 603 307 74
Fragments arranged by size:
603 307 267 74
BstYI r'GATC_y
Cuts at: 0 1173 1251
Size: 1173 78
BtgI C'CryG_G
Cuts at: 0 671 1251
Size: 671 580
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 673 1251
Size: 673 578
DraIII CAC_nnn'GTG
Cuts at: 0 1031 1251
Size: 1031 220
EaeI y'GGCC_r
Cuts at: 0 385 653 1046 1204 1251
Size: 385 268 393 158 47
Fragments arranged by size:
393 385 268 158 47
EagI C'GGCC_G
Cuts at: 0 385 653 1251
Size: 385 268 598
Fragments arranged by size:
598 385 268
EarI CTCTTCn'nnn_
Cuts at: 0 115 232 844 1251
Size: 115 117 612 407
Fragments arranged by size:
612 407 117 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 799 1251
Size: 799 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1251
Size: 643 608
EcoO109I rG'GnC_Cy
Cuts at: 0 271 272 422 434 507 906 1114 1136
Size: 271 1 150 12 73 399 208 22
Cuts at: 1136 1251
Size: 115
Fragments arranged by size:
399 271 208 150 115 73 22 12
1
HaeII r_GCGC'y
Cuts at: 0 492 604 671 1251
Size: 492 112 67 580
Fragments arranged by size:
580 492 112 67
KasI G'GCGC_C
Cuts at: 0 488 600 667 1251
Size: 488 112 67 584
Fragments arranged by size:
584 488 112 67
MslI CAynn'nnrTG
Cuts at: 0 1079 1251
Size: 1079 172
MspA1I CmG'CkG
Cuts at: 0 82 1251
Size: 82 1169
NaeI GCC'GGC
Cuts at: 0 739 1208 1251
Size: 739 469 43
Fragments arranged by size:
739 469 43
NarI GG'CG_CC
Cuts at: 0 489 601 668 1251
Size: 489 112 67 583
Fragments arranged by size:
583 489 112 67
NcoI C'CATG_G
Cuts at: 0 671 1251
Size: 671 580
NgoMIV G'CCGG_C
Cuts at: 0 737 1206 1251
Size: 737 469 45
Fragments arranged by size:
737 469 45
NotI GC'GGCC_GC
Cuts at: 0 653 1251
Size: 653 598
PasI CC'CwG_GG
Cuts at: 0 69 503 1251
Size: 69 434 748
Fragments arranged by size:
748 434 69
PpuMI rG'GwC_Cy
Cuts at: 0 906 1251
Size: 906 345
PspOMI G'GGCC_C
Cuts at: 0 271 506 1251
Size: 271 235 745
Fragments arranged by size:
745 271 235
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 799 831 1251
Size: 799 32 420
Fragments arranged by size:
799 420 32
PstI C_TGCA'G
Cuts at: 0 317 1251
Size: 317 934
PvuII CAG'CTG
Cuts at: 0 82 1251
Size: 82 1169
SapI GCTCTTCn'nnn_
Cuts at: 0 115 1251
Size: 115 1136
SexAI A'CCwGG_T
Cuts at: 0 788 818 1027 1251
Size: 788 30 209 224
Fragments arranged by size:
788 224 209 30
SfcI C'TryA_G
Cuts at: 0 313 1251
Size: 313 938
SfoI GGC'GCC
Cuts at: 0 490 602 669 1251
Size: 490 112 67 582
Fragments arranged by size:
582 490 112 67
StyI C'CwwG_G
Cuts at: 0 371 629 671 722 1132 1251
Size: 371 258 42 51 410 119
Fragments arranged by size:
410 371 258 119 51 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 652 941 1251
Size: 652 289 310
Fragments arranged by size:
652 310 289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 867 870 928 1251
Size: 867 3 58 323
Fragments arranged by size:
867 323 58 3
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI AvrII BamHI
BanI BanII BbeI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
HaeII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI PasI PpuMI PspOMI PsrI PstI PvuII SapI
SexAI SfcI SfoI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvaI
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI