(Circular) (Six-base) MAPSORT of: pF1KE2406.seq Check: 7916 from: 1 to: 7604 pF1KE2406 7604 bp With 182 enzymes: SgfI * April 20, 2012 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 372 1173 372 Size: 801 6803 AccI GT'mk_AC Cuts at: 4642 4642 Size: 7604 Acc65I G'GTAC_C Cuts at: 2479 4620 2479 Size: 2141 5463 AclI AA'CG_TT Cuts at: 7444 7444 Size: 7604 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 164 1357 1612 2938 3071 3380 5429 5861 6608 Size: 1193 255 1326 133 309 2049 432 747 Cuts at: 6608 6876 164 Size: 268 892 Fragments arranged by size: 2049 1326 1193 892 747 432 309 268 255 133 AflIII A'CryG_T Cuts at: 3077 3518 4956 6060 7037 3077 Size: 441 1438 1104 977 3644 Fragments arranged by size: 3644 1438 1104 977 441 AgeI A'CCGG_T Cuts at: 5992 5992 Size: 7604 AhdI GACnn_n'nnGTC Cuts at: 3711 3711 Size: 7604 AleI CACnn'nnGTG Cuts at: 1099 1099 Size: 7604 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1120 1152 1120 Size: 32 7572 AlwNI CAG_nnn'CTG Cuts at: 2095 2149 2626 3244 4909 6476 2095 Size: 54 477 618 1665 1567 3223 Fragments arranged by size: 3223 1665 1567 618 477 54 ApaI G_GGCC'C Cuts at: 1892 2140 3098 3107 3427 3566 3791 4306 4477 Size: 248 958 9 320 139 225 515 171 Cuts at: 4477 1892 Size: 5019 Fragments arranged by size: 5019 958 515 320 248 225 171 139 9 ApaLI G'TGCA_C Cuts at: 6374 6374 Size: 7604 ApoI r'AATT_y Cuts at: 481 530 639 3622 4608 6957 7213 481 Size: 49 109 2983 986 2349 256 872 Fragments arranged by size: 2983 2349 986 872 256 109 49 AseI AT'TA_AT Cuts at: 20 20 Size: 7604 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7604 AvaI C'yCGr_G Cuts at: 2258 2314 2741 3428 3558 3654 3782 3792 4514 Size: 56 427 687 130 96 128 10 722 Cuts at: 4514 4624 2258 Size: 110 5238 Fragments arranged by size: 5238 722 687 427 130 128 110 96 56 10 BamHI G'GATC_C Cuts at: 3668 4629 3668 Size: 961 6643 BanI G'GyrC_C Cuts at: 721 997 1472 1689 2479 2614 4081 4620 5034 Size: 276 475 217 790 135 1467 539 414 Cuts at: 5034 5282 5317 721 Size: 248 35 3008 Fragments arranged by size: 3008 1467 790 539 475 414 276 248 217 135 35 BanII G_rGCy'C Cuts at: 357 445 631 706 835 1751 1892 2140 2168 Size: 88 186 75 129 916 141 248 28 Cuts at: 2168 2315 2520 3027 3098 3107 3124 3427 3566 Size: 147 205 507 71 9 17 303 139 Cuts at: 3566 3599 3791 3810 4306 4477 4618 5648 357 Size: 33 192 19 496 171 141 1030 2313 Fragments arranged by size: 2313 1030 916 507 496 303 248 205 192 186 171 147 141 141 139 129 88 75 71 33 28 19 17 9 BbeI G_GCGC'C Cuts at: 5038 5286 5038 Size: 248 7356 BbsI GAAGACnn'nnnn_ Cuts at: 867 1268 1345 3368 4344 867 Size: 401 77 2023 976 4127 Fragments arranged by size: 4127 2023 976 401 77 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4110 4144 4110 Size: 34 7570 BciVI GTATCCnnnnn_n' Cuts at: 1891 5502 6263 7604 1891 Size: 3611 761 1341 1891 Fragments arranged by size: 3611 1891 1341 761 BclI T'GATC_A Cuts at: 3857 5124 3857 Size: 1267 6337 BglI GCCn_nnn'nGGC Cuts at: 1481 1682 1967 2135 2270 2630 2693 2712 2777 Size: 201 285 168 135 360 63 19 65 Cuts at: 2777 2831 2840 2975 3102 3111 3786 3908 4310 Size: 54 9 135 127 9 675 122 402 Cuts at: 4310 4329 1481 Size: 19 4756 Fragments arranged by size: 4756 675 402 360 285 201 168 135 135 127 122 65 63 54 19 19 9 9 BglII A'GATC_T Cuts at: 3190 4215 5119 7156 3190 Size: 1025 904 2037 3638 Fragments arranged by size: 3638 2037 1025 904 BlpI GC'TnA_GC Cuts at: 4716 4716 Size: 7604 Bme1580I G_kGCm'C Cuts at: 1477 1892 2140 2619 3098 3107 3427 3566 3791 Size: 415 248 479 479 9 320 139 225 Cuts at: 3791 4306 4450 4477 5229 5322 6378 1477 Size: 515 144 27 752 93 1056 2703 Fragments arranged by size: 2703 1056 752 515 479 479 415 320 248 225 144 139 93 27 9 BmgBI CAC'GTC Cuts at: 3393 3393 Size: 7604 BmrI ACTGGGnnnn_n' Cuts at: 702 2396 4816 4897 5232 7376 702 Size: 1694 2420 81 335 2144 930 Fragments arranged by size: 2420 2144 1694 930 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3618 3726 5971 3618 Size: 108 2245 5251 Fragments arranged by size: 5251 2245 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 283 429 1633 1663 1859 2048 2126 2444 2504 Size: 146 1204 30 196 189 78 318 60 Cuts at: 2504 2594 2606 3740 4232 4436 283 Size: 90 12 1134 492 204 3451 Fragments arranged by size: 3451 1204 1134 492 318 204 196 189 146 90 78 60 30 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 586 4780 5659 6151 6449 586 Size: 4194 879 492 298 1741 Fragments arranged by size: 4194 1741 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 676 1325 2161 2420 2449 2647 2855 7308 676 Size: 649 836 259 29 198 208 4453 972 Fragments arranged by size: 4453 972 836 649 259 208 198 29 BsaAI yAC'GTr Cuts at: 2701 3078 4957 2701 Size: 377 1879 5348 Fragments arranged by size: 5348 1879 377 BsaBI GATnn'nnATC Cuts at: 6 5118 7155 6 Size: 5112 2037 455 Fragments arranged by size: 5112 2037 455 BsaHI Gr'CG_yC Cuts at: 898 5035 5283 5985 7481 898 Size: 4137 248 702 1496 1021 Fragments arranged by size: 4137 1496 1021 702 248 BsaWI w'CCGG_w Cuts at: 462 3202 3845 4793 5314 5992 6266 6413 6824 Size: 2740 643 948 521 678 274 147 411 Cuts at: 6824 462 Size: 1242 Fragments arranged by size: 2740 1242 948 678 643 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 432 462 742 772 4566 4596 432 Size: 30 280 30 3794 30 3440 Fragments arranged by size: 3794 3440 280 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 832 930 1430 1591 1641 1759 3589 3607 3978 Size: 98 500 161 50 118 1830 18 371 Cuts at: 3978 3981 4064 832 Size: 3 83 4372 Fragments arranged by size: 4372 1830 500 371 161 118 98 83 50 18 3 BseYI C'CCAG_C Cuts at: 973 1512 2634 4480 4561 5029 5787 6364 6860 Size: 539 1122 1846 81 468 758 577 496 Cuts at: 6860 973 Size: 1717 Fragments arranged by size: 1846 1717 1122 758 577 539 496 468 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1074 1123 1711 4079 4094 1074 Size: 49 588 2368 15 4584 Fragments arranged by size: 4584 2368 588 49 15 BsiEI CG_ry'CG Cuts at: 104 616 5146 5192 6400 104 Size: 512 4530 46 1208 1308 Fragments arranged by size: 4530 1308 1208 512 46 BsiHKAI G_wGCw'C Cuts at: 445 706 835 1751 2520 3599 4045 4618 5396 Size: 261 129 916 769 1079 446 573 778 Cuts at: 5396 5586 6378 445 Size: 190 792 1671 Fragments arranged by size: 1671 1079 916 792 778 769 573 446 261 190 129 BsmI GAATG_Cn' Cuts at: 3032 3506 3032 Size: 474 7130 Bsp1286I G_dGCh'C Cuts at: 357 445 631 706 835 1477 1751 1892 2140 Size: 88 186 75 129 642 274 141 248 Cuts at: 2140 2168 2315 2520 2619 3027 3098 3107 3124 Size: 28 147 205 99 408 71 9 17 Cuts at: 3124 3427 3566 3599 3791 3810 4045 4306 4450 Size: 303 139 33 192 19 235 261 144 Cuts at: 4450 4477 4618 5229 5322 5396 5586 5648 6378 Size: 27 141 611 93 74 190 62 730 Cuts at: 6378 357 Size: 1583 Fragments arranged by size: 1583 730 642 611 408 303 274 261 248 235 205 192 190 186 147 144 141 141 139 129 99 93 88 75 74 71 62 33 28 27 19 17 9 BspEI T'CCGG_A Cuts at: 462 3202 3845 6824 462 Size: 2740 643 2979 1242 Fragments arranged by size: 2979 2740 1242 643 BspHI T'CATG_A Cuts at: 1193 6780 1193 Size: 5587 2017 BspMI ACCTGCnnnn'nnnn_ Cuts at: 372 1173 4654 4868 5170 5551 372 Size: 801 3481 214 302 381 2425 Fragments arranged by size: 3481 2425 801 381 302 214 BsrBI CCG'CTC Cuts at: 50 5896 5950 7434 7599 50 Size: 5846 54 1484 165 55 Fragments arranged by size: 5846 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 225 740 1562 2336 3684 4188 5516 225 Size: 515 822 774 1348 504 1328 2313 Fragments arranged by size: 2313 1348 1328 822 774 515 504 BsrFI r'CCGG_y Cuts at: 257 2629 3730 4596 5602 5783 5992 257 Size: 2372 1101 866 1006 181 209 1869 Fragments arranged by size: 2372 1869 1101 1006 866 209 181 BssSI C'ACGA_G Cuts at: 320 518 2000 5875 6233 320 Size: 198 1482 3875 358 1691 Fragments arranged by size: 3875 1691 1482 358 198 BstAPI GCAn_nnn'nTGC Cuts at: 3820 4081 4981 3820 Size: 261 900 6443 Fragments arranged by size: 6443 900 261 BstBI TT'CG_AA Cuts at: 5965 5965 Size: 7604 BstEII G'GTnAC_C Cuts at: 2236 4546 2236 Size: 2310 5294 BstXI CCAn_nnnn'nTGG Cuts at: 4405 4405 Size: 7604 BstYI r'GATC_y Cuts at: 1 1944 2023 3190 3668 4215 4629 5096 5119 Size: 1943 79 1167 478 547 414 467 23 Cuts at: 5119 5454 5700 6712 6798 6810 7156 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1943 1167 1012 547 478 467 449 414 346 335 246 86 79 23 12 Bsu36I CC'TnA_GG Cuts at: 2465 2925 2465 Size: 460 7144 BtgI C'CryG_G Cuts at: 107 785 797 993 1694 1964 1991 2403 2591 Size: 678 12 196 701 270 27 412 188 Cuts at: 2591 3956 107 Size: 1365 3755 Fragments arranged by size: 3755 1365 701 678 412 270 196 188 27 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1124 1224 5554 5735 5898 1124 Size: 100 4330 181 163 2830 Fragments arranged by size: 4330 2830 181 163 100 BtsI GCAGTG_nn' Cuts at: 1367 1401 1416 4847 1367 Size: 34 15 3431 4124 Fragments arranged by size: 4124 3431 34 15 ClaI AT'CG_AT Cuts at: 2551 2551 Size: 7604 DraI TTT'AAA Cuts at: 4603 4603 Size: 7604 DraIII CAC_nnn'GTG Cuts at: 2083 3300 2083 Size: 1217 6387 DrdI GACnn_nn'nnGTC Cuts at: 987 1922 3647 3767 5310 6168 987 Size: 935 1725 120 1543 858 2423 Fragments arranged by size: 2423 1725 1543 935 858 120 EaeI y'GGCC_r Cuts at: 2487 3138 5189 5363 5754 5781 7126 2487 Size: 651 2051 174 391 27 1345 2965 Fragments arranged by size: 2965 2051 1345 651 391 174 27 EagI C'GGCC_G Cuts at: 5189 5189 Size: 7604 EarI CTCTTCn'nnn_ Cuts at: 462 1558 3360 4038 5627 5837 462 Size: 1096 1802 678 1589 210 2229 Fragments arranged by size: 2229 1802 1589 1096 678 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1071 1588 3425 4154 4202 4581 5812 6122 6268 Size: 517 1837 729 48 379 1231 310 146 Cuts at: 6268 7414 1071 Size: 1146 1261 Fragments arranged by size: 1837 1261 1231 1146 729 517 379 310 146 48 EcoICRI GAG'CTC Cuts at: 443 704 833 1749 2518 3597 4616 443 Size: 261 129 916 769 1079 1019 3431 Fragments arranged by size: 3431 1079 1019 916 769 261 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 164 283 429 1357 1612 1633 1663 1859 2048 Size: 119 146 928 255 21 30 196 189 Cuts at: 2048 2126 2444 2504 2594 2606 2938 3071 3380 Size: 78 318 60 90 12 332 133 309 Cuts at: 3380 3740 4232 4436 5429 5861 6608 6876 164 Size: 360 492 204 993 432 747 268 892 Fragments arranged by size: 993 928 892 747 492 432 360 332 318 309 268 255 204 196 189 146 133 119 90 78 60 30 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 402 3050 402 Size: 2648 4956 EcoO109I rG'GnC_Cy Cuts at: 791 803 1843 1889 1960 1987 1996 2014 2065 Size: 12 1040 46 71 27 9 18 51 Cuts at: 2065 2101 2136 2137 2146 2227 2254 2335 2416 Size: 36 35 1 9 81 27 81 81 Cuts at: 2416 2452 2461 2569 2578 2596 2605 2623 2677 Size: 36 9 108 9 18 9 18 54 Cuts at: 2677 2713 2737 2851 2869 2878 3094 3095 3104 Size: 36 24 114 18 9 216 1 9 Cuts at: 3104 3214 3241 3424 3467 3562 3563 3649 3705 Size: 110 27 183 43 95 1 86 56 Cuts at: 3705 3751 3760 3778 3787 3788 3910 3928 4111 Size: 46 9 18 9 1 122 18 183 Cuts at: 4111 4269 4302 4420 4456 4473 4474 4743 791 Size: 158 33 118 36 17 1 269 3652 Fragments arranged by size: 3652 1040 269 216 183 183 158 122 118 114 110 108 95 86 81 81 81 71 56 54 51 46 46 43 36 36 36 36 35 33 27 27 27 24 18 18 18 18 18 18 17 12 9 9 9 9 9 9 9 9 9 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 639 3622 4608 639 Size: 2983 986 3635 Fragments arranged by size: 3635 2983 986 EcoRV GAT'ATC Cuts at: 1377 1377 Size: 7604 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 2680 5623 2200 Size: 480 2943 4181 Fragments arranged by size: 4181 2943 480 FspI TGC'GCA Cuts at: 3886 5385 3886 Size: 1499 6105 HaeII r_GCGC'y Cuts at: 1439 1465 5038 5286 6308 7287 1439 Size: 26 3573 248 1022 979 1756 Fragments arranged by size: 3573 1756 1022 979 248 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2533 2565 3411 3443 3697 3729 4434 4466 5446 Size: 32 846 32 254 32 705 32 980 Cuts at: 5446 5476 5478 5508 2533 Size: 30 2 30 4629 Fragments arranged by size: 4629 980 846 705 254 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 4643 4643 Size: 7604 KasI G'GCGC_C Cuts at: 5034 5282 5034 Size: 248 7356 KpnI G_GTAC'C Cuts at: 2483 4624 2483 Size: 2141 5463 MluI A'CGCG_T Cuts at: 7037 7037 Size: 7604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 671 2825 3618 6275 6459 671 Size: 2154 793 2657 184 1816 Fragments arranged by size: 2657 2154 1816 793 184 MscI TGG'CCA Cuts at: 3140 5365 3140 Size: 2225 5379 MslI CAynn'nnrTG Cuts at: 431 1099 3076 3697 5593 5720 431 Size: 668 1977 621 1896 127 2315 Fragments arranged by size: 2315 1977 1896 668 621 127 MspA1I CmG'CkG Cuts at: 293 787 1208 1496 1696 2117 2638 3384 3826 Size: 494 421 288 200 421 521 746 442 Cuts at: 3826 4715 5029 5389 6402 6647 293 Size: 889 314 360 1013 245 1250 Fragments arranged by size: 1250 1013 889 746 521 494 442 421 421 360 314 288 245 200 NaeI GCC'GGC Cuts at: 259 2631 3732 5785 259 Size: 2372 1101 2053 2078 Fragments arranged by size: 2372 2078 2053 1101 NarI GG'CG_CC Cuts at: 5035 5283 5035 Size: 248 7356 NcoI C'CATG_G Cuts at: 107 797 993 1964 1991 2591 107 Size: 690 196 971 27 600 5120 Fragments arranged by size: 5120 971 690 600 196 27 NdeI CA'TA_TG Cuts at: 1020 1020 Size: 7604 NgoMIV G'CCGG_C Cuts at: 257 2629 3730 5783 257 Size: 2372 1101 2053 2078 Fragments arranged by size: 2372 2078 2053 1101 NspI r_CATG'y Cuts at: 957 1041 3174 3522 4173 4260 4657 5157 6064 Size: 84 2133 348 651 87 397 500 907 Cuts at: 6064 957 Size: 2497 Fragments arranged by size: 2497 2133 907 651 500 397 348 87 84 PasI CC'CwG_GG Cuts at: 1867 1885 2457 2982 3100 3109 3775 3934 4068 Size: 18 572 525 118 9 666 159 134 Cuts at: 4068 4426 1867 Size: 358 5045 Fragments arranged by size: 5045 666 572 525 358 159 134 118 18 9 PciI A'CATG_T Cuts at: 3518 6060 3518 Size: 2542 5062 PflMI CCAn_nnn'nTGG Cuts at: 569 1686 569 Size: 1117 6487 PfoI T'CCnGG_A Cuts at: 740 2019 2583 3756 4452 740 Size: 1279 564 1173 696 3892 Fragments arranged by size: 3892 1279 1173 696 564 PmeI GTTT'AAAC Cuts at: 4603 4603 Size: 7604 PmlI CAC'GTG Cuts at: 2701 2701 Size: 7604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6743 6775 6743 Size: 32 7572 PpuMI rG'GwC_Cy Cuts at: 791 803 1996 2065 2101 2146 2227 2254 2335 Size: 12 1193 69 36 45 81 27 81 Cuts at: 2335 2416 2452 2461 2578 2596 2605 2677 2737 Size: 81 36 9 117 18 9 72 60 Cuts at: 2737 2851 2869 3214 3241 3467 3649 3705 3760 Size: 114 18 345 27 226 182 56 55 Cuts at: 3760 3928 4111 4420 4456 791 Size: 168 183 309 36 3939 Fragments arranged by size: 3939 1193 345 309 226 183 182 168 117 114 81 81 81 72 69 60 56 55 45 36 36 36 27 27 18 18 12 9 9 PshAI GACnn'nnGTC Cuts at: 2867 3395 4109 2867 Size: 528 714 6362 Fragments arranged by size: 6362 714 528 PspOMI G'GGCC_C Cuts at: 1888 2136 3094 3103 3423 3562 3787 4302 4473 Size: 248 958 9 320 139 225 515 171 Cuts at: 4473 1888 Size: 5019 Fragments arranged by size: 5019 958 515 320 248 225 171 139 9 PspXI vC'TCGA_Gb Cuts at: 2258 3782 4514 2258 Size: 1524 732 5348 Fragments arranged by size: 5348 1524 732 PstI C_TGCA'G Cuts at: 548 1374 1495 3383 4101 4651 5336 548 Size: 826 121 1888 718 550 685 2816 Fragments arranged by size: 2816 1888 826 718 685 550 121 PvuI CG_AT'CG Cuts at: 104 616 104 Size: 512 7092 PvuII CAG'CTG Cuts at: 1496 2638 3826 5029 5389 1496 Size: 1142 1188 1203 360 3711 Fragments arranged by size: 3711 1203 1188 1142 360 RsrII CG'GwC_CG Cuts at: 5799 5799 Size: 7604 SacI G_AGCT'C Cuts at: 445 706 835 1751 2520 3599 4618 445 Size: 261 129 916 769 1079 1019 3431 Fragments arranged by size: 3431 1079 1019 916 769 261 129 SacII CC_GC'GG Cuts at: 788 1697 788 Size: 909 6695 SalI G'TCGA_C Cuts at: 4641 4641 Size: 7604 SanDI GG'GwC_CC Cuts at: 2101 2596 3214 3467 3928 4111 2101 Size: 495 618 253 461 183 5594 Fragments arranged by size: 5594 618 495 461 253 183 SapI GCTCTTCn'nnn_ Cuts at: 1558 4038 5627 5837 1558 Size: 2480 1589 210 3325 Fragments arranged by size: 3325 2480 1589 210 SbfI CC_TGCA'GG Cuts at: 4651 4651 Size: 7604 ScaI AGT'ACT Cuts at: 3415 7121 3415 Size: 3706 3898 SexAI A'CCwGG_T Cuts at: 2061 2079 3228 4488 2061 Size: 18 1149 1260 5177 Fragments arranged by size: 5177 1260 1149 18 SfcI C'TryA_G Cuts at: 33 544 1370 1386 1491 2150 3379 4026 4097 Size: 511 826 16 105 659 1229 647 71 Cuts at: 4097 4362 4647 5332 6325 6516 33 Size: 265 285 685 993 191 1121 Fragments arranged by size: 1229 1121 993 826 685 659 647 511 285 265 191 105 71 16 SfiI GGCCn_nnn'nGGCC Cuts at: 1967 2135 2630 2693 2712 2777 2831 2840 2975 Size: 168 495 63 19 65 54 9 135 Cuts at: 2975 3102 3111 3786 3908 4310 4329 1967 Size: 127 9 675 122 402 19 5242 Fragments arranged by size: 5242 675 495 402 168 135 127 122 65 63 54 19 19 9 9 SfoI GGC'GCC Cuts at: 5036 5284 5036 Size: 248 7356 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7604 SmaI CCC'GGG Cuts at: 2316 3560 3656 3794 4626 2316 Size: 1244 96 138 832 5294 Fragments arranged by size: 5294 1244 832 138 96 SmlI C'TyrA_G Cuts at: 601 2258 3782 4514 4759 5674 6166 6428 601 Size: 1657 1524 732 245 915 492 262 1777 Fragments arranged by size: 1777 1657 1524 915 732 492 262 245 SpeI A'CTAG_T Cuts at: 67 67 Size: 7604 SphI G_CATG'C Cuts at: 957 4260 4657 5157 957 Size: 3303 397 500 3404 Fragments arranged by size: 3404 3303 500 397 StuI AGG'CCT Cuts at: 1310 2264 2906 1310 Size: 954 642 6008 Fragments arranged by size: 6008 954 642 StyI C'CwwG_G Cuts at: 107 797 993 1199 1450 1892 1964 1991 2160 Size: 690 196 206 251 442 72 27 169 Cuts at: 2160 2358 2591 2855 2865 2892 4738 5092 107 Size: 198 233 264 10 27 1846 354 2619 Fragments arranged by size: 2619 1846 690 442 354 264 251 233 206 198 196 169 72 27 27 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 924 1916 4060 5187 5320 5779 5988 924 Size: 992 2144 1127 133 459 209 2540 Fragments arranged by size: 2540 2144 1127 992 459 209 133 TatI w'GTAC_w Cuts at: 3155 3167 3413 7119 3155 Size: 12 246 3706 3640 Fragments arranged by size: 3706 3640 246 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 432 464 644 676 2069 2101 3653 3685 7121 Size: 32 180 32 1393 32 1552 32 3436 Cuts at: 7121 7153 432 Size: 32 883 Fragments arranged by size: 3436 1552 1393 883 180 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 509 1344 4552 5401 509 Size: 835 3208 849 2712 Fragments arranged by size: 3208 2712 849 835 XbaI T'CTAG_A Cuts at: 4635 4635 Size: 7604 XcmI CCAnnnn_n'nnnnTGG Cuts at: 566 1176 1466 3680 3696 4558 5099 566 Size: 610 290 2214 16 862 541 3071 Fragments arranged by size: 3071 2214 862 610 541 290 16 XhoI C'TCGA_G Cuts at: 2258 3782 4514 2258 Size: 1524 732 5348 Fragments arranged by size: 5348 1524 732 XmaI C'CCGG_G Cuts at: 2314 3558 3654 3792 4624 2314 Size: 1244 96 138 832 5294 Fragments arranged by size: 5294 1244 832 138 96 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AflII AlfI AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PsiI PsrI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI