################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HinfI HpaI HphI KpnI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 484 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 433 CCTCAGG CC^T[ACGT]AGG BfaI 616 CTAG C^TAG BglI 186 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 680 TACGTG [CT]AC^GT[GA] BsiEI 354 CGGCCG CG[GA][CT]^CG BsmBI 145 GAGACG CGTCTC(1/5) BsrBI 703 CCGCTC CCGCTC(-3/-3) BsrI 808 ACTGG ACTGG(1/-1) BssHII 413 GCGCGC G^CGCGC BstAPI 612 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 433 CCTCAGG CC^T[ACGT]AGG DpnII 138 GATC ^GATC DsaI 351 CCGCGG C^C[GA][CT]GG EagI 354 CGGCCG C^GGCCG EclXI 354 CGGCCG C^GGCCG Eco52I 354 CGGCCG C^GGCCG Eco81I 433 CCTCAGG CC^T[ACGT]AGG HindIII 688 AAGCTT A^AGCTT KspI 351 CCGCGG CCGC^GG MaeI 616 CTAG C^TAG MaeII 681 ACGT A^CGT MboI 138 GATC ^GATC NdeII 138 GATC ^GATC NlaIII 733 CATG CATG^ NotI 353 GCGGCCGC GC^GGCCGC NruI 664 TCGCGA TCG^CGA PvuII 154 CAGCTG CAG^CTG SacI 394 GAGCTC GAGCT^C SacII 351 CCGCGG CCGC^GG Sau3AI 138 GATC ^GATC SfaNI 476 GCATC GCATC(5/9) Tsp45I 773 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 394 GAGCTC G[AT]GC[AT]^C 619 GTGCTC BsiHKAI 394 GAGCTC G[AT]GC[AT]^C 619 GTGCTC BsmAI 145 GAGAC GTCTC(1/5) 301 GAGAC BstF5I 477 CATCC GGATG(2/0) 511 GGATG DdeI 434 CTCAG C^T[ACGT]AG 802 CTCAG EaeI 354 CGGCCG [CT]^GGCC[GA] 389 TGGCCG EarI 22 GAAGAG CTCTTC(1/4) 55 GAAGAG EcoNI 633 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 785 CCTCCGGGAGG FokI 477 CATCC GGATG(9/13) 511 GGATG Ksp632I 22 GAAGAG CTCTTC(1/4) 55 GAAGAG MaeIII 678 GTTAC ^GT[ACGT]AC 773 GTGAC MboII 22 GAAGA GAAGA(8/7) 55 GAAGA PshAI 554 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 713 GACCTCTGTC SmaI 198 CCCGGG CCC^GGG 219 CCCGGG XmaCI 198 CCCGGG C^CCGGG 219 CCCGGG XmaI 198 CCCGGG C^CCGGG 219 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIV PpuMI PstI Sau96I ScrFI SfcI SfoI TseI TspRI