(Circular) (Six-base) MAPSORT of: pF1KE1772.seq Check: 6000 from: 1 to: 3950 pF1KE1772 3950 bp With 182 enzymes: SgfI * October 24, 2019 20:49 .. AccI GT'mk_AC Cuts at: 988 988 Size: 3950 Acc65I G'GTAC_C Cuts at: 966 966 Size: 3950 AclI AA'CG_TT Cuts at: 3790 3790 Size: 3950 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1775 2207 2954 3222 1775 Size: 432 747 268 2503 Fragments arranged by size: 2503 747 432 268 AflIII A'CryG_T Cuts at: 1302 2406 3383 1302 Size: 1104 977 1869 Fragments arranged by size: 1869 1104 977 AgeI A'CCGG_T Cuts at: 2338 2338 Size: 3950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 806 806 Size: 3950 AlwNI CAG_nnn'CTG Cuts at: 597 1255 2822 597 Size: 658 1567 1725 Fragments arranged by size: 1725 1567 658 ApaI G_GGCC'C Cuts at: 305 327 335 421 305 Size: 22 8 86 3834 Fragments arranged by size: 3834 86 22 8 ApaLI G'TGCA_C Cuts at: 2720 2720 Size: 3950 ApoI r'AATT_y Cuts at: 954 3303 3559 954 Size: 2349 256 1345 Fragments arranged by size: 2349 1345 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3950 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3950 AvaI C'yCGr_G Cuts at: 216 306 327 970 216 Size: 90 21 643 3196 Fragments arranged by size: 3196 643 90 21 BamHI G'GATC_C Cuts at: 975 975 Size: 3950 BanI G'GyrC_C Cuts at: 312 348 378 423 631 889 966 1380 1628 Size: 36 30 45 208 258 77 414 248 Cuts at: 1628 1663 312 Size: 35 2599 Fragments arranged by size: 2599 414 258 248 208 77 45 36 35 30 BanII G_rGCy'C Cuts at: 305 327 335 421 506 964 1994 305 Size: 22 8 86 85 458 1030 2261 Fragments arranged by size: 2261 1030 458 86 85 22 8 BbeI G_GCGC'C Cuts at: 316 427 635 893 1384 1632 316 Size: 111 208 258 491 248 2634 Fragments arranged by size: 2634 491 258 248 208 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3916 BciVI GTATCCnnnnn_n' Cuts at: 1848 2609 3950 1848 Size: 761 1341 1848 Fragments arranged by size: 1848 1341 761 BclI T'GATC_A Cuts at: 1470 1470 Size: 3950 BglI GCCn_nnn'nGGC Cuts at: 300 300 Size: 3950 BglII A'GATC_T Cuts at: 1465 3502 1465 Size: 2037 1913 BlpI GC'TnA_GC Cuts at: 1062 1062 Size: 3950 Bme1580I G_kGCm'C Cuts at: 305 327 335 421 1575 1668 2724 305 Size: 22 8 86 1154 93 1056 1531 Fragments arranged by size: 1531 1154 1056 93 86 22 8 BmrI ACTGGGnnnn_n' Cuts at: 1162 1243 1578 3722 1162 Size: 81 335 2144 1390 Fragments arranged by size: 2144 1390 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 640 2317 640 Size: 1677 2273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1126 2005 2497 2795 1126 Size: 879 492 298 2281 Fragments arranged by size: 2281 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3654 3654 Size: 3950 BsaAI yAC'GTr Cuts at: 790 1303 790 Size: 513 3437 BsaBI GATnn'nnATC Cuts at: 6 1464 3501 6 Size: 1458 2037 455 Fragments arranged by size: 2037 1458 455 BsaHI Gr'CG_yC Cuts at: 313 424 632 890 1381 1629 2331 3827 313 Size: 111 208 258 491 248 702 1496 436 Fragments arranged by size: 1496 702 491 436 258 248 208 111 BsaWI w'CCGG_w Cuts at: 1139 1660 2338 2612 2759 3170 1139 Size: 521 678 274 147 411 1919 Fragments arranged by size: 1919 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 797 827 797 Size: 30 3920 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 139 160 181 184 139 Size: 21 21 3 3905 Fragments arranged by size: 3905 21 21 3 BseYI C'CCAG_C Cuts at: 207 517 1375 2133 2710 3206 207 Size: 310 858 758 577 496 951 Fragments arranged by size: 951 858 758 577 496 310 BsiEI CG_ry'CG Cuts at: 104 465 1492 1538 2746 104 Size: 361 1027 46 1208 1308 Fragments arranged by size: 1308 1208 1027 361 46 BsiHKAI G_wGCw'C Cuts at: 506 731 964 1742 1932 2724 506 Size: 225 233 778 190 792 1732 Fragments arranged by size: 1732 792 778 233 225 190 BsmBI CGTCTCn'nnnn_ Cuts at: 247 247 Size: 3950 Bsp1286I G_dGCh'C Cuts at: 305 327 335 421 506 731 964 1575 1668 Size: 22 8 86 85 225 233 611 93 Cuts at: 1668 1742 1932 1994 2724 305 Size: 74 190 62 730 1531 Fragments arranged by size: 1531 730 611 233 225 190 93 86 85 74 62 22 8 BspEI T'CCGG_A Cuts at: 3170 3170 Size: 3950 BspHI T'CATG_A Cuts at: 3126 3126 Size: 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1000 1214 1516 1897 1000 Size: 214 302 381 3053 Fragments arranged by size: 3053 381 302 214 BsrBI CCG'CTC Cuts at: 50 813 2242 2296 3780 3945 50 Size: 763 1429 54 1484 165 55 Fragments arranged by size: 1484 1429 763 165 55 54 BsrDI GCAATG_nn' Cuts at: 1862 1862 Size: 3950 BsrFI r'CCGG_y Cuts at: 139 230 351 611 707 1948 2129 2338 139 Size: 91 121 260 96 1241 181 209 1751 Fragments arranged by size: 1751 1241 260 209 181 121 96 91 BssHII G'CGCG_C Cuts at: 521 521 Size: 3950 BssSI C'ACGA_G Cuts at: 2221 2579 2221 Size: 358 3592 BstAPI GCAn_nnn'nTGC Cuts at: 726 1327 726 Size: 601 3349 BstBI TT'CG_AA Cuts at: 2311 2311 Size: 3950 BstYI r'GATC_y Cuts at: 1 975 1442 1465 1800 2046 3058 3144 3156 Size: 974 467 23 335 246 1012 86 12 Cuts at: 3156 3502 1 Size: 346 449 Fragments arranged by size: 1012 974 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 542 542 Size: 3950 BtgI C'CryG_G Cuts at: 459 459 Size: 3950 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1900 2081 2244 1900 Size: 181 163 3606 Fragments arranged by size: 3606 181 163 BtsI GCAGTG_nn' Cuts at: 532 885 1193 532 Size: 353 308 3289 Fragments arranged by size: 3289 353 308 DraI TTT'AAA Cuts at: 949 949 Size: 3950 DrdI GACnn_nn'nnGTC Cuts at: 1656 2514 1656 Size: 858 3092 EaeI y'GGCC_r Cuts at: 462 497 1535 1709 2100 2127 3472 462 Size: 35 1038 174 391 27 1345 940 Fragments arranged by size: 1345 1038 940 391 174 35 27 EagI C'GGCC_G Cuts at: 462 1535 462 Size: 1073 2877 EarI CTCTTCn'nnn_ Cuts at: 125 158 1973 2183 125 Size: 33 1815 210 1892 Fragments arranged by size: 1892 1815 210 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 166 370 421 2158 2468 2614 3760 166 Size: 204 51 1737 310 146 1146 356 Fragments arranged by size: 1737 1146 356 310 204 146 51 EcoICRI GAG'CTC Cuts at: 504 962 504 Size: 458 3492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1775 2207 2954 3222 1775 Size: 432 747 268 2503 Fragments arranged by size: 2503 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 745 897 745 Size: 152 3798 EcoO109I rG'GnC_Cy Cuts at: 302 324 331 332 368 435 514 668 820 Size: 22 7 1 36 67 79 154 152 Cuts at: 820 1089 302 Size: 269 3163 Fragments arranged by size: 3163 269 154 152 79 67 36 22 7 1 EcoRI G'AATT_C Cuts at: 954 954 Size: 3950 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1969 1969 Size: 3950 FspI TGC'GCA Cuts at: 1731 1731 Size: 3950 HaeII r_GCGC'y Cuts at: 208 316 427 635 893 1384 1632 2654 3633 Size: 108 111 208 258 491 248 1022 979 Cuts at: 3633 208 Size: 525 Fragments arranged by size: 1022 979 525 491 258 248 208 111 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1792 1822 1824 1854 1792 Size: 30 2 30 3888 Fragments arranged by size: 3888 30 30 2 HincII GTy'rAC Cuts at: 989 989 Size: 3950 HindIII A'AGCT_T Cuts at: 796 796 Size: 3950 KasI G'GCGC_C Cuts at: 312 423 631 889 1380 1628 312 Size: 111 208 258 491 248 2634 Fragments arranged by size: 2634 491 258 248 208 111 KpnI G_GTAC'C Cuts at: 970 970 Size: 3950 MluI A'CGCG_T Cuts at: 3383 3383 Size: 3950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2621 2805 2621 Size: 184 3766 MscI TGG'CCA Cuts at: 1711 1711 Size: 3950 MslI CAynn'nnrTG Cuts at: 1939 2066 1939 Size: 127 3823 MspA1I CmG'CkG Cuts at: 264 318 384 461 597 1061 1375 1735 2748 Size: 54 66 77 136 464 314 360 1013 Cuts at: 2748 2993 264 Size: 245 1221 Fragments arranged by size: 1221 1013 464 360 314 245 136 77 66 54 NaeI GCC'GGC Cuts at: 141 353 613 2131 141 Size: 212 260 1518 1960 Fragments arranged by size: 1960 1518 260 212 NarI GG'CG_CC Cuts at: 313 424 632 890 1381 1629 313 Size: 111 208 258 491 248 2634 Fragments arranged by size: 2634 491 258 248 208 111 NgoMIV G'CCGG_C Cuts at: 139 351 611 2129 139 Size: 212 260 1518 1960 Fragments arranged by size: 1960 1518 260 212 NotI GC'GGCC_GC Cuts at: 462 462 Size: 3950 NruI TCG'CGA Cuts at: 774 774 Size: 3950 NspI r_CATG'y Cuts at: 1003 1503 2410 1003 Size: 500 907 2543 Fragments arranged by size: 2543 907 500 PasI CC'CwG_GG Cuts at: 430 450 430 Size: 20 3930 PciI A'CATG_T Cuts at: 2406 2406 Size: 3950 PfoI T'CCnGG_A Cuts at: 564 895 564 Size: 331 3619 PmeI GTTT'AAAC Cuts at: 949 949 Size: 3950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3089 3121 3089 Size: 32 3918 PpuMI rG'GwC_Cy Cuts at: 368 435 668 820 368 Size: 67 233 152 3498 Fragments arranged by size: 3498 233 152 67 PshAI GACnn'nnGTC Cuts at: 666 825 666 Size: 159 3791 PspOMI G'GGCC_C Cuts at: 301 323 331 417 301 Size: 22 8 86 3834 Fragments arranged by size: 3834 86 22 8 PstI C_TGCA'G Cuts at: 194 263 656 719 785 880 997 1682 194 Size: 69 393 63 66 95 117 685 2462 Fragments arranged by size: 2462 685 393 117 95 69 66 63 PvuI CG_AT'CG Cuts at: 104 104 Size: 3950 PvuII CAG'CTG Cuts at: 264 1375 1735 264 Size: 1111 360 2479 Fragments arranged by size: 2479 1111 360 RsrII CG'GwC_CG Cuts at: 2145 2145 Size: 3950 SacI G_AGCT'C Cuts at: 506 964 506 Size: 458 3492 SacII CC_GC'GG Cuts at: 462 462 Size: 3950 SalI G'TCGA_C Cuts at: 987 987 Size: 3950 SanDI GG'GwC_CC Cuts at: 668 668 Size: 3950 SapI GCTCTTCn'nnn_ Cuts at: 1973 2183 1973 Size: 210 3740 SbfI CC_TGCA'GG Cuts at: 997 997 Size: 3950 ScaI AGT'ACT Cuts at: 3467 3467 Size: 3950 SfcI C'TryA_G Cuts at: 33 190 259 652 715 781 876 993 1678 Size: 157 69 393 63 66 95 117 685 Cuts at: 1678 2671 2862 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 685 393 191 157 117 95 69 66 63 SfoI GGC'GCC Cuts at: 314 425 633 891 1382 1630 314 Size: 111 208 258 491 248 2634 Fragments arranged by size: 2634 491 258 248 208 111 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3950 SmaI CCC'GGG Cuts at: 308 329 972 308 Size: 21 643 3286 Fragments arranged by size: 3286 643 21 SmlI C'TyrA_G Cuts at: 1105 2020 2512 2774 1105 Size: 915 492 262 2281 Fragments arranged by size: 2281 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3950 SphI G_CATG'C Cuts at: 1003 1503 1003 Size: 500 3450 StyI C'CwwG_G Cuts at: 1084 1438 1084 Size: 354 3596 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1533 1666 2125 2334 1533 Size: 133 459 209 3149 Fragments arranged by size: 3149 459 209 133 TatI w'GTAC_w Cuts at: 3465 3465 Size: 3950 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3467 3499 3467 Size: 32 3918 Tth111I GACn'n_nGTC Cuts at: 1747 1747 Size: 3950 XbaI T'CTAG_A Cuts at: 981 981 Size: 3950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1445 1445 Size: 3950 XmaI C'CCGG_G Cuts at: 306 327 970 306 Size: 21 643 3286 Fragments arranged by size: 3286 643 21 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NruI NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI NsiI PacI PflMI PmlI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI