Restriction map for pF1KE1772
 (Linear) (Six-base) MAPSORT of: KIEE1772.seq  Check: 8700  from: 1  to: 837



KIEE1772.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     698     837 
   Size:        698     139
 
AlwNI CAG_nnn'CTG

Cuts at:      0     489     837 
   Size:        489     348
 
ApaI G_GGCC'C

Cuts at:      0     197     219     227     313     837 
   Size:        197      22       8      86     524

  Fragments arranged by size: 

                 524     197      86      22       8
 
AvaI C'yCGr_G

Cuts at:      0     108     198     219     837 
   Size:        108      90      21     618

  Fragments arranged by size: 

                 618     108      90      21
 
BanI G'GyrC_C

Cuts at:      0     204     240     270     315     523     781     837 
   Size:        204      36      30      45     208     258      56

  Fragments arranged by size: 

                 258     208     204      56      45      36      30
 
BanII G_rGCy'C

Cuts at:      0     197     219     227     313     398     837 
   Size:        197      22       8      86      85     439

  Fragments arranged by size: 

                 439     197      86      85      22       8
 
BbeI G_GCGC'C

Cuts at:      0     208     319     527     785     837 
   Size:        208     111     208     258      52

  Fragments arranged by size: 

                 258     208     208     111      52
 
BglI GCCn_nnn'nGGC

Cuts at:      0     192     837 
   Size:        192     645
 
Bme1580I G_kGCm'C

Cuts at:      0     197     219     227     313     837 
   Size:        197      22       8      86     524

  Fragments arranged by size: 

                 524     197      86      22       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     532     837 
   Size:        532     305
 
BsaAI yAC'GTr

Cuts at:      0     682     837 
   Size:        682     155
 
BsaHI Gr'CG_yC

Cuts at:      0     205     316     524     782     837 
   Size:        205     111     208     258      55

  Fragments arranged by size: 

                 258     208     205     111      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     689     719     837 
   Size:        689      30     118

  Fragments arranged by size: 

                 689     118      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31      52      73      76     837 
   Size:         31      21      21       3     761

  Fragments arranged by size: 

                 761      31      21      21       3
 
BseYI C'CCAG_C

Cuts at:      0      99     409     837 
   Size:         99     310     428

  Fragments arranged by size: 

                 428     310      99
 
BsiEI CG_ry'CG

Cuts at:      0     357     837 
   Size:        357     480
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     623     837 
   Size:        398     225     214

  Fragments arranged by size: 

                 398     225     214
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     139     837 
   Size:        139     698
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     219     227     313     398     623     837 
   Size:        197      22       8      86      85     225     214

  Fragments arranged by size: 

                 225     214     197      86      85      22       8
 
BsrBI CCG'CTC

Cuts at:      0     705     837 
   Size:        705     132
 
BsrFI r'CCGG_y

Cuts at:      0      31     122     243     503     599     837 
   Size:         31      91     121     260      96     238

  Fragments arranged by size: 

                 260     238     121      96      91      31
 
BssHII G'CGCG_C

Cuts at:      0     413     837 
   Size:        413     424
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     618     837 
   Size:        618     219
 
Bsu36I CC'TnA_GG

Cuts at:      0     434     837 
   Size:        434     403
 
BtgI C'CryG_G

Cuts at:      0     351     837 
   Size:        351     486
 
BtsI GCAGTG_nn'

Cuts at:      0     424     777     837 
   Size:        424     353      60

  Fragments arranged by size: 

                 424     353      60
 
EaeI y'GGCC_r

Cuts at:      0     354     389     837 
   Size:        354      35     448

  Fragments arranged by size: 

                 448     354      35
 
EagI C'GGCC_G

Cuts at:      0     354     837 
   Size:        354     483
 
EarI CTCTTCn'nnn_

Cuts at:      0      17      50     837 
   Size:         17      33     787

  Fragments arranged by size: 

                 787      33      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     262     313     837 
   Size:         58     204      51     524

  Fragments arranged by size: 

                 524     204      58      51
 
EcoICRI GAG'CTC

Cuts at:      0     396     837 
   Size:        396     441
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     637     789     837 
   Size:        637     152      48

  Fragments arranged by size: 

                 637     152      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     216     223     224     260     327     406     560 
   Size:        194      22       7       1      36      67      79     154

Cuts at:    560     712     837 
   Size:        152     125

  Fragments arranged by size: 

                 194     154     152     125      79      67      36      22
                   7       1
 
HaeII r_GCGC'y

Cuts at:      0     100     208     319     527     785     837 
   Size:        100     108     111     208     258      52

  Fragments arranged by size: 

                 258     208     111     108     100      52
 
HindIII A'AGCT_T

Cuts at:      0     688     837 
   Size:        688     149
 
KasI G'GCGC_C

Cuts at:      0     204     315     523     781     837 
   Size:        204     111     208     258      56

  Fragments arranged by size: 

                 258     208     204     111      56
 
MspA1I CmG'CkG

Cuts at:      0     156     210     276     353     489     837 
   Size:        156      54      66      77     136     348

  Fragments arranged by size: 

                 348     156     136      77      66      54
 
NaeI GCC'GGC

Cuts at:      0      33     245     505     837 
   Size:         33     212     260     332

  Fragments arranged by size: 

                 332     260     212      33
 
NarI GG'CG_CC

Cuts at:      0     205     316     524     782     837 
   Size:        205     111     208     258      55

  Fragments arranged by size: 

                 258     208     205     111      55
 
NgoMIV G'CCGG_C

Cuts at:      0      31     243     503     837 
   Size:         31     212     260     334

  Fragments arranged by size: 

                 334     260     212      31
 
NotI GC'GGCC_GC

Cuts at:      0     354     837 
   Size:        354     483
 
NruI TCG'CGA

Cuts at:      0     666     837 
   Size:        666     171
 
PasI CC'CwG_GG

Cuts at:      0     322     342     837 
   Size:        322      20     495

  Fragments arranged by size: 

                 495     322      20
 
PfoI T'CCnGG_A

Cuts at:      0     456     787     837 
   Size:        456     331      50

  Fragments arranged by size: 

                 456     331      50
 
PpuMI rG'GwC_Cy

Cuts at:      0     260     327     560     712     837 
   Size:        260      67     233     152     125

  Fragments arranged by size: 

                 260     233     152     125      67
 
PshAI GACnn'nnGTC

Cuts at:      0     558     717     837 
   Size:        558     159     120

  Fragments arranged by size: 

                 558     159     120
 
PspOMI G'GGCC_C

Cuts at:      0     193     215     223     309     837 
   Size:        193      22       8      86     528

  Fragments arranged by size: 

                 528     193      86      22       8
 
PstI C_TGCA'G

Cuts at:      0      86     155     548     611     677     772     837 
   Size:         86      69     393      63      66      95      65

  Fragments arranged by size: 

                 393      95      86      69      66      65      63
 
PvuII CAG'CTG

Cuts at:      0     156     837 
   Size:        156     681
 
SacI G_AGCT'C

Cuts at:      0     398     837 
   Size:        398     439
 
SacII CC_GC'GG

Cuts at:      0     354     837 
   Size:        354     483
 
SanDI GG'GwC_CC

Cuts at:      0     560     837 
   Size:        560     277
 
SfcI C'TryA_G

Cuts at:      0      82     151     544     607     673     768     837 
   Size:         82      69     393      63      66      95      69

  Fragments arranged by size: 

                 393      95      82      69      69      66      63
 
SfoI GGC'GCC

Cuts at:      0     206     317     525     783     837 
   Size:        206     111     208     258      54

  Fragments arranged by size: 

                 258     208     206     111      54
 
SmaI CCC'GGG

Cuts at:      0     200     221     837 
   Size:        200      21     616

  Fragments arranged by size: 

                 616     200      21
 
XmaI C'CCGG_G

Cuts at:      0     198     219     837 
   Size:        198      21     618

  Fragments arranged by size: 

                 618     198      21
 

 Enzymes that do cut:

     AlfI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BglI
 Bme1580I     BplI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrFI   BssHII   BstAPI   Bsu36I
     BtgI     BtsI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    HaeII  HindIII     KasI   MspA1I     NaeI     NarI   NgoMIV
     NotI     NruI     PasI     PfoI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI    SacII    SanDI     SfcI     SfoI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NheI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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