Restriction map for pF1KB8468
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaII       HphI        ItaI        
KasI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TseI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Acc65I         37  GGTACC               G^GTACC
AccI           62  GTAGAC               GT^[AC][GT]AC
Asp700I       124  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        37  GGTACC               G^GTACC
BalI          682  TGGCCA               TGG^CCA
BbsI          194  GTCTTC               GAAGAC(2/6)
BclI          630  TGATCA               T^GATCA
BglII         508  AGATCT               A^GATCT
BmyI          660  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         194  GTCTTC               GAAGAC(2/6)
BsmAI         380  GAGAC                GTCTC(1/5)
Bsp1286I      660  GGGCAC               G[AGT]GC[ACT]^C
DdeI          596  CTAAG                C^T[ACGT]AG
EaeI          682  TGGCCA               [CT]^GGCC[GA]
FbaI          630  TGATCA               T^GATCA
HpaI          801  GTTAAC               GTT^AAC
KpnI           37  GGTACC               GGTAC^C
MluNI         682  TGGCCA               TGG^CCA
MscI          682  TGGCCA               TGG^CCA
MwoI          583  GCTGAACTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
ScaI          136  AGTACT               AGT^ACT
SfaNI         748  GCATC                GCATC(5/9)
TaqI          793  TCGA                 T^CGA
Tsp45I        356  GTCAC                ^GT[GC]AC
TspRI         842  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       793  TCGA                 T^CGA
XbaI          361  TCTAGA               T^CTAGA
XcmI          550  CCAGTTGACATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          124  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          152  AAATTT               [GA]^AATT[CT]
              824  GAATTT              
AlwI          811  GATCC                GGATC(4/5)
              255  GGATC               
ApoI          152  AAATTT               [GA]^AATT[CT]
              824  GAATTT              
BanI           37  GGTACC               G^G[CT][GA]CC
              571  GGCACC              
BpmI          512  CTCCAG               CTGGAG(16/14)
              548  CTCCAG              
BsaJI         251  CCATGG               C^C[ACGT][ACGT]GG
              612  CCATGG              
BsrI          429  ACTGG                ACTGG(1/-1)
              550  CCAGT               
BstYI         255  GGATCT               [GA]^GATC[CT]
              508  AGATCT              
DsaI          251  CCATGG               C^C[GA][CT]GG
              612  CCATGG              
EcoT14I       251  CCATGG               C^C[AT][AT]GG
              612  CCATGG              
HaeIII        442  GGCC                 GG^CC
              683  GGCC                
HincII        553  GTTGAC               GT[CT]^[GA]AC
              801  GTTAAC              
HindII        553  GTTGAC               GT[CT]^[GA]AC
              801  GTTAAC              
MflI          255  GGATCT               [GA]^GATC[CT]
              508  AGATCT              
NcoI          251  CCATGG               C^CATGG
              612  CCATGG              
SfcI           74  CTACAG               C^T[GA][CT]AG
              337  CTACAG              
StyI          251  CCATGG               C^C[AT][AT]GG
              612  CCATGG              
XhoII         255  GGATCT               [GA]^GATC[CT]
              508  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BfaI        BssKI       BstF5I      
BstNI       DpnII       EarI        EcoRII      FokI        
HinfI       Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      ScrFI       
TfiI        Tru9I       Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com