Restriction map for
pF1KE6787
(Linear) (Six-base) MAPSORT of: KIEE6787.seq Check: 1436 from: 1 to: 2073
KIEE6787.orf
With 182 enzymes: SgfI *
August 9, 2016 17:39 ..
AfeI AGC'GCT
Cuts at: 0 1157 2073
Size: 1157 916
AflII C'TTAA_G
Cuts at: 0 1776 2073
Size: 1776 297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1673 2073
Size: 1673 400
AlwNI CAG_nnn'CTG
Cuts at: 0 1428 1909 2073
Size: 1428 481 164
Fragments arranged by size:
1428 481 164
ApoI r'AATT_y
Cuts at: 0 300 421 1300 1815 2073
Size: 300 121 879 515 258
Fragments arranged by size:
879 515 300 258 121
AseI AT'TA_AT
Cuts at: 0 1351 2073
Size: 1351 722
AvrII C'CTAG_G
Cuts at: 0 2019 2073
Size: 2019 54
BanI G'GyrC_C
Cuts at: 0 33 218 2073
Size: 33 185 1855
Fragments arranged by size:
1855 185 33
BanII G_rGCy'C
Cuts at: 0 1141 2073
Size: 1141 932
BbsI GAAGACnn'nnnn_
Cuts at: 0 1804 2073
Size: 1804 269
BbvCI CC'TCA_GC
Cuts at: 0 87 1033 2073
Size: 87 946 1040
Fragments arranged by size:
1040 946 87
BciVI GTATCCnnnnn_n'
Cuts at: 0 36 2073
Size: 36 2037
BclI T'GATC_A
Cuts at: 0 632 852 2073
Size: 632 220 1221
Fragments arranged by size:
1221 632 220
BglII A'GATC_T
Cuts at: 0 380 2073
Size: 380 1693
Bme1580I G_kGCm'C
Cuts at: 0 223 1448 2073
Size: 223 1225 625
Fragments arranged by size:
1225 625 223
BmrI ACTGGGnnnn_n'
Cuts at: 0 224 1144 2073
Size: 224 920 929
Fragments arranged by size:
929 920 224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 359 2073
Size: 359 1714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 634 1111 2073
Size: 314 320 477 962
Fragments arranged by size:
962 477 320 314
Bpu10I CC'TnA_GC
Cuts at: 0 87 1033 2073
Size: 87 946 1040
Fragments arranged by size:
1040 946 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 2073
Size: 165 1908
BsaAI yAC'GTr
Cuts at: 0 1449 2073
Size: 1449 624
BsaBI GATnn'nnATC
Cuts at: 0 254 1726 2073
Size: 254 1472 347
Fragments arranged by size:
1472 347 254
BsaWI w'CCGG_w
Cuts at: 0 8 2073
Size: 8 2065
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 754 1021 1195 2073
Size: 243 511 267 174 878
Fragments arranged by size:
878 511 267 243 174
BseYI C'CCAG_C
Cuts at: 0 1146 1912 2073
Size: 1146 766 161
Fragments arranged by size:
1146 766 161
BsiHKAI G_wGCw'C
Cuts at: 0 1775 2073
Size: 1775 298
BsiWI C'GTAC_G
Cuts at: 0 727 2073
Size: 727 1346
BsmI GAATG_Cn'
Cuts at: 0 245 2073
Size: 245 1828
Bsp1286I G_dGCh'C
Cuts at: 0 223 1141 1448 1775 2073
Size: 223 918 307 327 298
Fragments arranged by size:
918 327 307 298 223
BspEI T'CCGG_A
Cuts at: 0 8 2073
Size: 8 2065
BspHI T'CATG_A
Cuts at: 0 1625 1737 2073
Size: 1625 112 336
Fragments arranged by size:
1625 336 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 934 2073
Size: 143 791 1139
Fragments arranged by size:
1139 791 143
BsrFI r'CCGG_y
Cuts at: 0 126 2073
Size: 126 1947
BstAPI GCAn_nnn'nTGC
Cuts at: 0 260 2073
Size: 260 1813
BstEII G'GTnAC_C
Cuts at: 0 157 2073
Size: 157 1916
BstXI CCAn_nnnn'nTGG
Cuts at: 0 914 2073
Size: 914 1159
BstYI r'GATC_y
Cuts at: 0 380 1895 2073
Size: 380 1515 178
Fragments arranged by size:
1515 380 178
Bsu36I CC'TnA_GG
Cuts at: 0 448 1265 2073
Size: 448 817 808
Fragments arranged by size:
817 808 448
BtgI C'CryG_G
Cuts at: 0 108 223 881 1365 2073
Size: 108 115 658 484 708
Fragments arranged by size:
708 658 484 115 108
BtsI GCAGTG_nn'
Cuts at: 0 594 1907 2073
Size: 594 1313 166
Fragments arranged by size:
1313 594 166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1704 1739 2073
Size: 1704 35 334
Fragments arranged by size:
1704 334 35
EaeI y'GGCC_r
Cuts at: 0 1220 2073
Size: 1220 853
EarI CTCTTCn'nnn_
Cuts at: 0 350 371 879 2073
Size: 350 21 508 1194
Fragments arranged by size:
1194 508 350 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 730 2073
Size: 730 1343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 634 1111 2073
Size: 314 320 477 962
Fragments arranged by size:
962 477 320 314
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 451 2073
Size: 139 312 1622
Fragments arranged by size:
1622 312 139
EcoO109I rG'GnC_Cy
Cuts at: 0 969 1425 2073
Size: 969 456 648
Fragments arranged by size:
969 648 456
EcoRV GAT'ATC
Cuts at: 0 498 2073
Size: 498 1575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 331 1011 2073
Size: 331 680 1062
Fragments arranged by size:
1062 680 331
HaeII r_GCGC'y
Cuts at: 0 1159 2073
Size: 1159 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 2073
Size: 70 32 1971
Fragments arranged by size:
1971 70 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1613 2073
Size: 185 1428 460
Fragments arranged by size:
1428 460 185
MslI CAynn'nnrTG
Cuts at: 0 1175 1313 1810 2073
Size: 1175 138 497 263
Fragments arranged by size:
1175 497 263 138
MspA1I CmG'CkG
Cuts at: 0 945 1037 1206 1558 1873 2073
Size: 945 92 169 352 315 200
Fragments arranged by size:
945 352 315 200 169 92
NcoI C'CATG_G
Cuts at: 0 881 1365 2073
Size: 881 484 708
Fragments arranged by size:
881 708 484
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 1303 2073
Size: 546 757 770
Fragments arranged by size:
770 757 546
PmlI CAC'GTG
Cuts at: 0 1449 2073
Size: 1449 624
PsiI TTA'TAA
Cuts at: 0 1822 2073
Size: 1822 251
PstI C_TGCA'G
Cuts at: 0 566 2073
Size: 566 1507
PvuII CAG'CTG
Cuts at: 0 945 1037 1206 1558 1873 2073
Size: 945 92 169 352 315 200
Fragments arranged by size:
945 352 315 200 169 92
ScaI AGT'ACT
Cuts at: 0 520 789 2073
Size: 520 269 1284
Fragments arranged by size:
1284 520 269
SfcI C'TryA_G
Cuts at: 0 139 562 961 1104 1188 1568 2073
Size: 139 423 399 143 84 380 505
Fragments arranged by size:
505 423 399 380 143 139 84
SmlI C'TyrA_G
Cuts at: 0 180 1776 2073
Size: 180 1596 297
Fragments arranged by size:
1596 297 180
SspI AAT'ATT
Cuts at: 0 2064 2073
Size: 2064 9
StyI C'CwwG_G
Cuts at: 0 881 1055 1365 1934 2019 2073
Size: 881 174 310 569 85 54
Fragments arranged by size:
881 569 310 174 85 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 179 206 2073
Size: 21 158 27 1867
Fragments arranged by size:
1867 158 27 21
TatI w'GTAC_w
Cuts at: 0 518 787 2073
Size: 518 269 1286
Fragments arranged by size:
1286 518 269
Tth111I GACn'n_nGTC
Cuts at: 0 1233 2073
Size: 1233 840
XmnI GAAnn'nnTTC
Cuts at: 0 1946 2073
Size: 1946 127
Enzymes that do cut:
AfeI AflII AlfI AlwNI ApoI AseI AvrII BanI
BanII BbsI BbvCI BciVI BclI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI
BseYI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I MmeI MslI MspA1I NcoI PflMI
PmlI PsiI PstI PvuII ScaI SfcI SmlI SspI
StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
BaeI BamHI BbeI BcgI BfrBI BglI BlpI BmgBI
BmtI BsaI BsaHI BsaXI BsgI BsiEI BsmBI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EcoICRI EcoRI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI XbaI XcmI XhoI XmaI ZraI