Restriction map for
pF1KE2518
(Circular) (Six-base) MAPSORT of: pF1KE2518.seq Check: 5199 from: 1 to: 7481
pF1KE2518 7481 bp
With 182 enzymes: SgfI *
February 23, 2015 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 509 1285 509
Size: 776 6705
AccI GT'mk_AC
Cuts at: 577 4519 577
Size: 3942 3539
Acc65I G'GTAC_C
Cuts at: 4497 4497
Size: 7481
AclI AA'CG_TT
Cuts at: 1438 7321 1438
Size: 5883 1598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2528 5306 5738 6485 6753 2258
Size: 270 2778 432 747 268 2986
Fragments arranged by size:
2986 2778 747 432 270 268
AfeI AGC'GCT
Cuts at: 208 208
Size: 7481
AflII C'TTAA_G
Cuts at: 1776 1776
Size: 7481
AflIII A'CryG_T
Cuts at: 2753 3161 3561 4350 4833 5937 6914 2753
Size: 408 400 789 483 1104 977 3320
Fragments arranged by size:
3320 1104 977 789 483 408 400
AgeI A'CCGG_T
Cuts at: 5869 5869
Size: 7481
AhdI GACnn_n'nnGTC
Cuts at: 3187 3187
Size: 7481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1739 1771 2283 2315 3149 3181 1739
Size: 32 512 32 834 32 6039
Fragments arranged by size:
6039 834 512 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1113 2506 3128 4786 6353 1113
Size: 1393 622 1658 1567 2241
Fragments arranged by size:
2241 1658 1567 1393 622
ApaLI G'TGCA_C
Cuts at: 4354 6251 4354
Size: 1897 5584
ApoI r'AATT_y
Cuts at: 277 394 742 1149 1324 1875 1972 2598 2820
Size: 117 348 407 175 551 97 626 222
Cuts at: 2820 2955 3445 4485 6834 7090 277
Size: 135 490 1040 2349 256 668
Fragments arranged by size:
2349 1040 668 626 551 490 407 348
256 222 175 135 117 97
AseI AT'TA_AT
Cuts at: 20 2212 2408 20
Size: 2192 196 5093
Fragments arranged by size:
5093 2192 196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7481
AvaI C'yCGr_G
Cuts at: 2135 4501 2135
Size: 2366 5115
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2874 2907 2874
Size: 33 7448
BamHI G'GATC_C
Cuts at: 3510 4506 3510
Size: 996 6485
BanI G'GyrC_C
Cuts at: 827 2059 4372 4497 4911 5159 5194 827
Size: 1232 2313 125 414 248 35 3114
Fragments arranged by size:
3114 2313 1232 414 248 125 35
BanII G_rGCy'C
Cuts at: 869 2154 3659 4495 5525 869
Size: 1285 1505 836 1030 2825
Fragments arranged by size:
2825 1505 1285 1030 836
BbeI G_GCGC'C
Cuts at: 2063 4376 4915 5163 2063
Size: 2313 539 248 4381
Fragments arranged by size:
4381 2313 539 248
BbsI GAAGACnn'nnnn_
Cuts at: 1742 3712 3889 4112 4253 1742
Size: 1970 177 223 141 4970
Fragments arranged by size:
4970 1970 223 177 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1281 1315 2570 2604 1281
Size: 34 1255 34 6158
Fragments arranged by size:
6158 1255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1318 5379 6140 7481 1318
Size: 4061 761 1341 1318
Fragments arranged by size:
4061 1341 1318 761
BclI T'GATC_A
Cuts at: 2702 5001 2702
Size: 2299 5182
BfrBI ATG'CAT
Cuts at: 3526 3526
Size: 7481
BglII A'GATC_T
Cuts at: 527 4434 4996 7033 527
Size: 3907 562 2037 975
Fragments arranged by size:
3907 2037 975 562
BlpI GC'TnA_GC
Cuts at: 863 4593 863
Size: 3730 3751
Bme1580I G_kGCm'C
Cuts at: 4358 5106 5199 6255 4358
Size: 748 93 1056 5584
Fragments arranged by size:
5584 1056 748 93
BmgBI CAC'GTC
Cuts at: 4025 4351 4025
Size: 326 7155
BmrI ACTGGGnnnn_n'
Cuts at: 224 363 1123 1533 1998 2331 3143 3213 3651
Size: 139 760 410 465 333 812 70 438
Cuts at: 3651 3882 4693 4774 5109 7253 224
Size: 231 811 81 335 2144 452
Fragments arranged by size:
2144 812 811 760 465 452 438 410
335 333 231 139 81 70
BmtI G_CTAG'C
Cuts at: 2174 2174
Size: 7481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1283 5848 1283
Size: 4565 2916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 3362 540
Size: 2822 4659
Bpu10I CC'TnA_GC
Cuts at: 871 2468 871
Size: 1597 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 4349 4657 5536 6028 6326 2471
Size: 1878 308 879 492 298 3626
Fragments arranged by size:
3626 1878 879 492 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 3066 4056 7185 3066
Size: 990 3129 3362
Fragments arranged by size:
3362 3129 990
BsaAI yAC'GTr
Cuts at: 2754 4834 2754
Size: 2080 5401
BsaBI GATnn'nnATC
Cuts at: 6 4995 7032 6
Size: 4989 2037 455
Fragments arranged by size:
4989 2037 455
BsaHI Gr'CG_yC
Cuts at: 2060 4373 4912 5160 5862 7358 2060
Size: 2313 539 248 702 1496 2183
Fragments arranged by size:
2313 2183 1496 702 539 248
BsaWI w'CCGG_w
Cuts at: 2046 4047 4670 5191 5869 6143 6290 6701 2046
Size: 2001 623 521 678 274 147 411 2826
Fragments arranged by size:
2826 2001 678 623 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 2013 2043 2283 2313 2466 2496 3360
Size: 30 1876 30 240 30 153 30 864
Cuts at: 3360 3390 107
Size: 30 4198
Fragments arranged by size:
4198 1876 864 240 153 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2474 3200 2474
Size: 726 6755
BseYI C'CCAG_C
Cuts at: 661 1343 4906 5664 6241 6737 661
Size: 682 3563 758 577 496 1405
Fragments arranged by size:
3563 1405 758 682 577 496
BsiEI CG_ry'CG
Cuts at: 104 670 5023 5069 6277 104
Size: 566 4353 46 1208 1308
Fragments arranged by size:
4353 1308 1208 566 46
BsiHKAI G_wGCw'C
Cuts at: 556 869 2154 4358 4495 5273 5463 6255 556
Size: 313 1285 2204 137 778 190 792 1782
Fragments arranged by size:
2204 1782 1285 792 778 313 190 137
BsmI GAATG_Cn'
Cuts at: 613 1719 3503 613
Size: 1106 1784 4591
Fragments arranged by size:
4591 1784 1106
Bsp1286I G_dGCh'C
Cuts at: 556 869 2154 3659 4358 4495 5106 5199 5273
Size: 313 1285 1505 699 137 611 93 74
Cuts at: 5273 5463 5525 6255 556
Size: 190 62 730 1782
Fragments arranged by size:
1782 1505 1285 730 699 611 313 190
137 93 74 62
BspEI T'CCGG_A
Cuts at: 2046 6701 2046
Size: 4655 2826
BspHI T'CATG_A
Cuts at: 888 3434 4206 6657 888
Size: 2546 772 2451 1712
Fragments arranged by size:
2546 2451 1712 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 1285 3325 4531 4745 5047 5428 509
Size: 776 2040 1206 214 302 381 2562
Fragments arranged by size:
2562 2040 1206 776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5773 5827 7311 7476 50
Size: 5723 54 1484 165 55
Fragments arranged by size:
5723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 597 3714 3726 5393 597
Size: 3117 12 1667 2685
Fragments arranged by size:
3117 2685 1667 12
BsrFI r'CCGG_y
Cuts at: 1216 5479 5660 5869 1216
Size: 4263 181 209 2828
Fragments arranged by size:
4263 2828 209 181
BssSI C'ACGA_G
Cuts at: 654 4438 5752 6110 654
Size: 3784 1314 358 2025
Fragments arranged by size:
3784 2025 1314 358
BstAPI GCAn_nnn'nTGC
Cuts at: 610 2680 4858 610
Size: 2070 2178 3233
Fragments arranged by size:
3233 2178 2070
BstBI TT'CG_AA
Cuts at: 3900 5842 3900
Size: 1942 5539
BstEII G'GTnAC_C
Cuts at: 1016 2380 3214 1016
Size: 1364 834 5283
Fragments arranged by size:
5283 1364 834
BstXI CCAn_nnnn'nTGG
Cuts at: 3507 3507
Size: 7481
BstYI r'GATC_y
Cuts at: 1 527 3510 3687 4077 4434 4506 4973 4996
Size: 526 2983 177 390 357 72 467 23
Cuts at: 4996 5331 5577 6589 6675 6687 7033 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2983 1012 526 467 449 390 357 346
335 246 177 86 72 23 12
BstZ17I GTA'TAC
Cuts at: 578 578
Size: 7481
Bsu36I CC'TnA_GG
Cuts at: 930 930
Size: 7481
BtgI C'CryG_G
Cuts at: 2567 3831 2567
Size: 1264 6217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5431 5612 5775 5431
Size: 181 163 7137
Fragments arranged by size:
7137 181 163
BtsI GCAGTG_nn'
Cuts at: 227 759 985 1299 4724 227
Size: 532 226 314 3425 2984
Fragments arranged by size:
3425 2984 532 314 226
DraI TTT'AAA
Cuts at: 321 1148 4480 321
Size: 827 3332 3322
Fragments arranged by size:
3332 3322 827
DrdI GACnn_nn'nnGTC
Cuts at: 953 5187 6045 953
Size: 4234 858 2389
Fragments arranged by size:
4234 2389 858
EaeI y'GGCC_r
Cuts at: 1207 4271 5066 5240 5631 5658 7003 1207
Size: 3064 795 174 391 27 1345 1685
Fragments arranged by size:
3064 1685 1345 795 391 174 27
EagI C'GGCC_G
Cuts at: 5066 5066
Size: 7481
EarI CTCTTCn'nnn_
Cuts at: 1847 5504 5714 1847
Size: 3657 210 3614
Fragments arranged by size:
3657 3614 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5689 5999 6145 7291 5689
Size: 310 146 1146 5879
Fragments arranged by size:
5879 1146 310 146
EcoICRI GAG'CTC
Cuts at: 867 2152 4493 867
Size: 1285 2341 3855
Fragments arranged by size:
3855 2341 1285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 2258 2528 3362 5306 5738 6485 6753 540
Size: 1718 270 834 1944 432 747 268 1268
Fragments arranged by size:
1944 1718 1268 834 747 432 270 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2276
Size: 7481
EcoO109I rG'GnC_Cy
Cuts at: 1427 4620 1427
Size: 3193 4288
EcoRI G'AATT_C
Cuts at: 1324 1875 4485 1324
Size: 551 2610 4320
Fragments arranged by size:
4320 2610 551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3109 5500 3109
Size: 2391 5090
FspI TGC'GCA
Cuts at: 5262 5262
Size: 7481
HaeII r_GCGC'y
Cuts at: 210 2063 4376 4915 5163 6185 7164 210
Size: 1853 2313 539 248 1022 979 527
Fragments arranged by size:
2313 1853 1022 979 539 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 2662 2694 2740 2772 3173 3205 3360
Size: 32 2377 32 46 32 401 32 155
Cuts at: 3360 3392 3582 3614 4268 4300 5323 5353 5355
Size: 32 190 32 654 32 1023 30 2
Cuts at: 5355 5385 253
Size: 30 2349
Fragments arranged by size:
2377 2349 1023 654 401 190 155 46
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4062 4520 4062
Size: 458 7023
HindIII A'AGCT_T
Cuts at: 759 2076 3032 3478 3881 759
Size: 1317 956 446 403 4359
Fragments arranged by size:
4359 1317 956 446 403
KasI G'GCGC_C
Cuts at: 2059 4372 4911 5159 2059
Size: 2313 539 248 4381
Fragments arranged by size:
4381 2313 539 248
KpnI G_GTAC'C
Cuts at: 4501 4501
Size: 7481
MluI A'CGCG_T
Cuts at: 6914 6914
Size: 7481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 2932 3380 4289 6152 6336 650
Size: 2282 448 909 1863 184 1795
Fragments arranged by size:
2282 1863 1795 909 448 184
MscI TGG'CCA
Cuts at: 5242 5242
Size: 7481
MslI CAynn'nnrTG
Cuts at: 1241 2908 2937 3531 3719 4288 5470 5597 1241
Size: 1667 29 594 188 569 1182 127 3125
Fragments arranged by size:
3125 1667 1182 594 569 188 127 29
MspA1I CmG'CkG
Cuts at: 780 971 1036 4592 4906 5266 6279 6524 780
Size: 191 65 3556 314 360 1013 245 1737
Fragments arranged by size:
3556 1737 1013 360 314 245 191 65
NaeI GCC'GGC
Cuts at: 5662 5662
Size: 7481
NarI GG'CG_CC
Cuts at: 2060 4373 4912 5160 2060
Size: 2313 539 248 4381
Fragments arranged by size:
4381 2313 539 248
NcoI C'CATG_G
Cuts at: 2567 3831 2567
Size: 1264 6217
NdeI CA'TA_TG
Cuts at: 2262 2313 3340 2262
Size: 51 1027 6403
Fragments arranged by size:
6403 1027 51
NgoMIV G'CCGG_C
Cuts at: 5660 5660
Size: 7481
NheI G'CTAG_C
Cuts at: 2170 2170
Size: 7481
NsiI A_TGCA'T
Cuts at: 3528 3528
Size: 7481
NspI r_CATG'y
Cuts at: 3677 4117 4534 5034 5941 3677
Size: 440 417 500 907 5217
Fragments arranged by size:
5217 907 500 440 417
PciI A'CATG_T
Cuts at: 5937 5937
Size: 7481
PflMI CCAn_nnn'nTGG
Cuts at: 3992 3992
Size: 7481
PfoI T'CCnGG_A
Cuts at: 1068 1068
Size: 7481
PmeI GTTT'AAAC
Cuts at: 4480 4480
Size: 7481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1846 1878 2283 2315 6620 6652 1846
Size: 32 405 32 4305 32 2675
Fragments arranged by size:
4305 2675 405 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1427 1427
Size: 7481
PshAI GACnn'nnGTC
Cuts at: 992 992
Size: 7481
PsiI TTA'TAA
Cuts at: 4340 4340
Size: 7481
PstI C_TGCA'G
Cuts at: 1282 1621 3542 3939 4528 5213 1282
Size: 339 1921 397 589 685 3550
Fragments arranged by size:
3550 1921 685 589 397 339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7481
PvuII CAG'CTG
Cuts at: 1036 4906 5266 1036
Size: 3870 360 3251
Fragments arranged by size:
3870 3251 360
RsrII CG'GwC_CG
Cuts at: 5676 5676
Size: 7481
SacI G_AGCT'C
Cuts at: 869 2154 4495 869
Size: 1285 2341 3855
Fragments arranged by size:
3855 2341 1285
SalI G'TCGA_C
Cuts at: 4518 4518
Size: 7481
SanDI GG'GwC_CC
Cuts at: 1427 1427
Size: 7481
SapI GCTCTTCn'nnn_
Cuts at: 5504 5714 5504
Size: 210 7271
SbfI CC_TGCA'GG
Cuts at: 1282 4528 1282
Size: 3246 4235
ScaI AGT'ACT
Cuts at: 6998 6998
Size: 7481
SfcI C'TryA_G
Cuts at: 33 1278 1617 2961 3516 3538 3935 4524 5209
Size: 1245 339 1344 555 22 397 589 685
Cuts at: 5209 6202 6393 33
Size: 993 191 1121
Fragments arranged by size:
1344 1245 1121 993 685 589 555 397
339 191 22
SfoI GGC'GCC
Cuts at: 2061 4374 4913 5161 2061
Size: 2313 539 248 4381
Fragments arranged by size:
4381 2313 539 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7481
SmaI CCC'GGG
Cuts at: 4503 4503
Size: 7481
SmlI C'TyrA_G
Cuts at: 1776 2135 2486 4364 4636 5551 6043 6305 1776
Size: 359 351 1878 272 915 492 262 2952
Fragments arranged by size:
2952 1878 915 492 359 351 272 262
SpeI A'CTAG_T
Cuts at: 67 1762 4420 67
Size: 1695 2658 3128
Fragments arranged by size:
3128 2658 1695
SphI G_CATG'C
Cuts at: 4534 5034 4534
Size: 500 6981
SspI AAT'ATT
Cuts at: 486 1792 4140 4449 486
Size: 1306 2348 309 3518
Fragments arranged by size:
3518 2348 1306 309
StuI AGG'CCT
Cuts at: 1782 1782
Size: 7481
StyI C'CwwG_G
Cuts at: 1584 2567 3831 4615 4969 1584
Size: 983 1264 784 354 4096
Fragments arranged by size:
4096 1264 983 784 354
SwaI ATTT'AAAT
Cuts at: 321 321
Size: 7481
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 1882 2024 2146 2504 2916 4458 5064 5197
Size: 1606 142 122 358 412 1542 606 133
Cuts at: 5197 5656 5865 276
Size: 459 209 1892
Fragments arranged by size:
1892 1606 1542 606 459 412 358 209
142 133 122
TatI w'GTAC_w
Cuts at: 1406 6996 1406
Size: 5590 1891
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 2013 2045 2589 2621 2729 2761 4062
Size: 32 1718 32 544 32 108 32 1301
Cuts at: 4062 4086 4094 4118 4135 4167 6998 7030 263
Size: 24 8 24 17 32 2831 32 714
Fragments arranged by size:
2831 1718 1301 714 544 108 32 32
32 32 32 32 24 24 17 8
Tth111I GACn'n_nGTC
Cuts at: 5278 5278
Size: 7481
XbaI T'CTAG_A
Cuts at: 4512 4512
Size: 7481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4976 4976
Size: 7481
XhoI C'TCGA_G
Cuts at: 2135 2135
Size: 7481
XmaI C'CCGG_G
Cuts at: 4501 4501
Size: 7481
XmnI GAAnn'nnTTC
Cuts at: 1103 1856 2271 3896 3906 1103
Size: 753 415 1625 10 4678
Fragments arranged by size:
4678 1625 753 415 10
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI ApaI AscI AvrII BbvCI BglI
BsgI BsiWI BsmBI BsrGI BssHII ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NotI NruI PacI
PasI PmlI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI ZraI