Restriction map for pF1KE3319
 (Linear) (Six-base) MAPSORT of: KIEE3319.seq  Check: 22  from: 1  to: 2667



KIEE3319.orf

 With 182 enzymes: SgfI * 

                        March 2, 2011 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1032    2401    2592    2667 
   Size:       1032    1369     191      75

  Fragments arranged by size: 

                1369    1032     191      75
 
Acc65I G'GTAC_C

Cuts at:      0    1209    2667 
   Size:       1209    1458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     373     481     785    1651    1789    2074    2113 
   Size:        112     261     108     304     866     138     285      39

Cuts at:   2113    2436    2667 
   Size:        323     231

  Fragments arranged by size: 

                 866     323     304     285     261     231     138     112
                 108      39
 
AflIII A'CryG_T

Cuts at:      0     386    2667 
   Size:        386    2281
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2643    2667 
   Size:       2643      24
 
AleI CACnn'nnGTG

Cuts at:      0     959    2667 
   Size:        959    1708
 
AlwNI CAG_nnn'CTG

Cuts at:      0     165     168     444     526     684     717     873    1221 
   Size:        165       3     276      82     158      33     156     348

Cuts at:   1221    2667 
   Size:       1446

  Fragments arranged by size: 

                1446     348     276     165     158     156      82      33
                   3
 
AvaI C'yCGr_G

Cuts at:      0     761    1401    1969    2182    2404    2667 
   Size:        761     640     568     213     222     263

  Fragments arranged by size: 

                 761     640     568     263     222     213
 
AvrII C'CTAG_G

Cuts at:      0    1609    2667 
   Size:       1609    1058
 
BamHI G'GATC_C

Cuts at:      0    1826    2667 
   Size:       1826     841
 
BanI G'GyrC_C

Cuts at:      0      12     314     400     586     780    1209    1364    1853 
   Size:         12     302      86     186     194     429     155     489

Cuts at:   1853    2161    2667 
   Size:        308     506

  Fragments arranged by size: 

                 506     489     429     308     302     194     186     155
                  86      12
 
BanII G_rGCy'C

Cuts at:      0     312    1095    1976    2667 
   Size:        312     783     881     691

  Fragments arranged by size: 

                 881     783     691     312
 
BbeI G_GCGC'C

Cuts at:      0      16     590    1368    2667 
   Size:         16     574     778    1299

  Fragments arranged by size: 

                1299     778     574      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1504    1956    2667 
   Size:       1504     452     711

  Fragments arranged by size: 

                1504     711     452
 
BbvCI CC'TCA_GC

Cuts at:      0     581     647    1201    2667 
   Size:        581      66     554    1466

  Fragments arranged by size: 

                1466     581     554      66
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     781    2667 
   Size:        781    1886
 
BglI GCCn_nnn'nGGC

Cuts at:      0     149     857     910    2667 
   Size:        149     708      53    1757

  Fragments arranged by size: 

                1757     708     149      53
 
BlpI GC'TnA_GC

Cuts at:      0     263    1683    1786    2667 
   Size:        263    1420     103     881

  Fragments arranged by size: 

                1420     881     263     103
 
Bme1580I G_kGCm'C

Cuts at:      0    1856    2272    2392    2667 
   Size:       1856     416     120     275

  Fragments arranged by size: 

                1856     416     275     120
 
BmgBI CAC'GTC

Cuts at:      0    1621    2667 
   Size:       1621    1046
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1472    2667 
   Size:       1472    1195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     517     739    1105    1123    1852    1878    2365 
   Size:        211     306     222     366      18     729      26     487

Cuts at:   2365    2667 
   Size:        302

  Fragments arranged by size: 

                 729     487     366     306     302     222     211      26
                  18
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     527     581     647    1201    2667 
   Size:        304     223      54      66     554    1466

  Fragments arranged by size: 

                1466     554     304     223      66      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    2380    2667 
   Size:       1183    1197     287

  Fragments arranged by size: 

                1197    1183     287
 
BsaAI yAC'GTr

Cuts at:      0     389    1992    2667 
   Size:        389    1603     675

  Fragments arranged by size: 

                1603     675     389
 
BsaBI GATnn'nnATC

Cuts at:      0     484    1948    2667 
   Size:        484    1464     719

  Fragments arranged by size: 

                1464     719     484
 
BsaHI Gr'CG_yC

Cuts at:      0      13     587    1365    2324    2667 
   Size:         13     574     778     959     343

  Fragments arranged by size: 

                 959     778     574     343      13
 
BsaWI w'CCGG_w

Cuts at:      0     329    2141    2667 
   Size:        329    1812     526

  Fragments arranged by size: 

                1812     526     329
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     674     704    2667 
   Size:        674      30    1963

  Fragments arranged by size: 

                1963     674      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     556    1588    2368    2443    2590    2667 
   Size:         19     537    1032     780      75     147      77

  Fragments arranged by size: 

                1032     780     537     147      77      75      19
 
BseYI C'CCAG_C

Cuts at:      0     258     434     659     794     897    1034    1692    2232 
   Size:        258     176     225     135     103     137     658     540

Cuts at:   2232    2667 
   Size:        435

  Fragments arranged by size: 

                 658     540     435     258     225     176     137     135
                 103
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     205     544    1830    2570    2667 
   Size:        178      27     339    1286     740      97

  Fragments arranged by size: 

                1286     740     339     178      97      27
 
BsiEI CG_ry'CG

Cuts at:      0     289     905    2667 
   Size:        289     616    1762

  Fragments arranged by size: 

                1762     616     289
 
BsiHKAI G_wGCw'C

Cuts at:      0    1408    1787    2667 
   Size:       1408     379     880

  Fragments arranged by size: 

                1408     880     379
 
Bsp1286I G_dGCh'C

Cuts at:      0     312    1095    1408    1787    1856    1976    2272    2392 
   Size:        312     783     313     379      69     120     296     120

Cuts at:   2392    2667 
   Size:        275

  Fragments arranged by size: 

                 783     379     313     312     296     275     120     120
                  69
 
BspEI T'CCGG_A

Cuts at:      0     329    2667 
   Size:        329    2338
 
BspHI T'CATG_A

Cuts at:      0    1373    1940    2667 
   Size:       1373     567     727

  Fragments arranged by size: 

                1373     727     567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     834    1032    1388    2401    2540    2592    2667 
   Size:        125     709     198     356    1013     139      52      75

  Fragments arranged by size: 

                1013     709     356     198     139     125      75      52
            
 
BssSI C'ACGA_G

Cuts at:      0    1954    2026    2667 
   Size:       1954      72     641

  Fragments arranged by size: 

                1954     641      72
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     165     168     444     558    1221    1782    2475    2667 
   Size:        165       3     276     114     663     561     693     192

  Fragments arranged by size: 

                 693     663     561     276     192     165     114       3
            
 
BstEII G'GTnAC_C

Cuts at:      0    1236    1933    2667 
   Size:       1236     697     734

  Fragments arranged by size: 

                1236     734     697
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     597    2667 
   Size:        597    2070
 
BstYI r'GATC_y

Cuts at:      0    1826    1949    2667 
   Size:       1826     123     718

  Fragments arranged by size: 

                1826     718     123
 
Bsu36I CC'TnA_GG

Cuts at:      0     276    1324    2667 
   Size:        276    1048    1343

  Fragments arranged by size: 

                1343    1048     276
 
BtgI C'CryG_G

Cuts at:      0      16     289    2667 
   Size:         16     273    2378

  Fragments arranged by size: 

                2378     273      16
 
BtsI GCAGTG_nn'

Cuts at:      0     838    1447    2603    2667 
   Size:        838     609    1156      64

  Fragments arranged by size: 

                1156     838     609      64
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2311    2667 
   Size:       2311     356
 
EaeI y'GGCC_r

Cuts at:      0     128     286     902     911     963    1046    2667 
   Size:        128     158     616       9      52      83    1621

  Fragments arranged by size: 

                1621     616     158     128      83      52       9
 
EagI C'GGCC_G

Cuts at:      0     286     902    2667 
   Size:        286     616    1765

  Fragments arranged by size: 

                1765     616     286
 
EarI CTCTTCn'nnn_

Cuts at:      0    1508    2288    2667 
   Size:       1508     780     379

  Fragments arranged by size: 

                1508     780     379
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     553     838    2667 
   Size:         58     495     285    1829

  Fragments arranged by size: 

                1829     495     285      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     211     373     481     517     739     785    1105 
   Size:        112      99     162     108      36     222      46     320

Cuts at:   1105    1123    1651    1789    1852    1878    2074    2113    2365 
   Size:         18     528     138      63      26     196      39     252

Cuts at:   2365    2436    2667 
   Size:         71     231

  Fragments arranged by size: 

                 528     320     252     231     222     196     162     138
                 112     108      99      71      63      46      39      36
                  26      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1555    2667 
   Size:       1555    1112
 
EcoO109I rG'GnC_Cy

Cuts at:      0     280     509     523    1716    2049    2667 
   Size:        280     229      14    1193     333     618

  Fragments arranged by size: 

                1193     618     333     280     229      14
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1521    2667 
   Size:       1521    1146
 
FspI TGC'GCA

Cuts at:      0     748    2667 
   Size:        748    1919
 
HaeII r_GCGC'y

Cuts at:      0      16     590     894    1368    2403    2667 
   Size:         16     574     304     474    1035     264

  Fragments arranged by size: 

                1035     574     474     304     264      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1552    1584    2629    2661    2667 
   Size:       1552      32    1045      32       6

  Fragments arranged by size: 

                1552    1045      32      32       6
 
HincII GTy'rAC

Cuts at:      0    2316    2667 
   Size:       2316     351
 
KasI G'GCGC_C

Cuts at:      0      12     586    1364    2667 
   Size:         12     574     778    1303

  Fragments arranged by size: 

                1303     778     574      12
 
KpnI G_GTAC'C

Cuts at:      0    1213    2667 
   Size:       1213    1454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    2039    2667 
   Size:        203    1836     628

  Fragments arranged by size: 

                1836     628     203
 
MscI TGG'CCA

Cuts at:      0     130     913    2667 
   Size:        130     783    1754

  Fragments arranged by size: 

                1754     783     130
 
MslI CAynn'nnrTG

Cuts at:      0     959    1958    2667 
   Size:        959     999     709

  Fragments arranged by size: 

                 999     959     709
 
MspA1I CmG'CkG

Cuts at:      0      18     165     198     262     339     430     459     574 
   Size:         18     147      33      64      77      91      29     115

Cuts at:    574     714     901    1679    2667 
   Size:        140     187     778     988

  Fragments arranged by size: 

                 988     778     187     147     140     115      91      77
                  64      33      29      18
 
NarI GG'CG_CC

Cuts at:      0      13     587    1365    2667 
   Size:         13     574     778    1302

  Fragments arranged by size: 

                1302     778     574      13
 
NdeI CA'TA_TG

Cuts at:      0    1601    2667 
   Size:       1601    1066
 
NotI GC'GGCC_GC

Cuts at:      0     902    2667 
   Size:        902    1765
 
NspI r_CATG'y

Cuts at:      0     346    2667 
   Size:        346    2321
 
PasI CC'CwG_GG

Cuts at:      0     247    2082    2278    2667 
   Size:        247    1835     196     389

  Fragments arranged by size: 

                1835     389     247     196
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2465    2667 
   Size:       2465     202
 
PfoI T'CCnGG_A

Cuts at:      0    1172    1829    2627    2657    2667 
   Size:       1172     657     798      30      10

  Fragments arranged by size: 

                1172     798     657      30      10
 
PmlI CAC'GTG

Cuts at:      0     389    2667 
   Size:        389    2278
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2257    2289    2536    2568    2667 
   Size:       2257      32     247      32      99

  Fragments arranged by size: 

                2257     247      99      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     280     509    1716    2049    2667 
   Size:        280     229    1207     333     618

  Fragments arranged by size: 

                1207     618     333     280     229
 
PshAI GACnn'nnGTC

Cuts at:      0    1072    2667 
   Size:       1072    1595
 
PstI C_TGCA'G

Cuts at:      0     845    1280    1396    2018    2667 
   Size:        845     435     116     622     649

  Fragments arranged by size: 

                 845     649     622     435     116
 
PvuII CAG'CTG

Cuts at:      0     165     198     262     339     430     459     574     714 
   Size:        165      33      64      77      91      29     115     140

Cuts at:    714    2667 
   Size:       1953

  Fragments arranged by size: 

                1953     165     140     115      91      77      64      33
                  29
 
SacII CC_GC'GG

Cuts at:      0      19    2667 
   Size:         19    2648
 
SanDI GG'GwC_CC

Cuts at:      0     509    2667 
   Size:        509    2158
 
SfcI C'TryA_G

Cuts at:      0     547     664     841    1276    1392    2014    2667 
   Size:        547     117     177     435     116     622     653

  Fragments arranged by size: 

                 653     622     547     435     177     117     116
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     910    2667 
   Size:        910    1757
 
SfoI GGC'GCC

Cuts at:      0      14     588    1366    2667 
   Size:         14     574     778    1301

  Fragments arranged by size: 

                1301     778     574      14
 
SmlI C'TyrA_G

Cuts at:      0    1162    2359    2667 
   Size:       1162    1197     308

  Fragments arranged by size: 

                1197    1162     308
 
StuI AGG'CCT

Cuts at:      0    1459    2667 
   Size:       1459    1208
 
StyI C'CwwG_G

Cuts at:      0     407     704     944    1505    1609    2667 
   Size:        407     297     240     561     104    1058

  Fragments arranged by size: 

                1058     561     407     297     240     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1498    1545    2224    2251    2667 
   Size:       1498      47     679      27     416

  Fragments arranged by size: 

                1498     679     416      47      27
 
TatI w'GTAC_w

Cuts at:      0    1811    2667 
   Size:       1811     856
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     830     862    1180    1212    1578    1605    1610    1637 
   Size:        830      32     318      32     366      27       5      27

Cuts at:   1637    2667 
   Size:       1030

  Fragments arranged by size: 

                1030     830     366     318      32      32      27      27
                   5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     186     987    2667 
   Size:        186     801    1680

  Fragments arranged by size: 

                1680     801     186
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AhdI     AleI    AlwNI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NarI     NdeI     NotI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI     PstI    PvuII    SacII    SanDI
     SfcI     SfiI     SfoI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AlfI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI     BcgI
     BclI    BfrBI    BglII     BmtI     BplI     BsaI    BsiWI     BsmI
    BsmBI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoRI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     MluI     NaeI     NcoI
   NgoMIV     NheI     NruI     NsiI     PacI     PciI     PmeI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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