Restriction map for
pF1KE6507
(Linear) (Six-base) MAPSORT of: KIEE6507.seq Check: 7142 from: 1 to: 630
KIEE6507.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AflIII A'CryG_T
Cuts at: 0 353 630
Size: 353 277
AhdI GACnn_n'nnGTC
Cuts at: 0 537 630
Size: 537 93
ApoI r'AATT_y
Cuts at: 0 228 630
Size: 228 402
AvaI C'yCGr_G
Cuts at: 0 10 74 630
Size: 10 64 556
Fragments arranged by size:
556 64 10
BanI G'GyrC_C
Cuts at: 0 14 630
Size: 14 616
BanII G_rGCy'C
Cuts at: 0 569 630
Size: 569 61
BbeI G_GCGC'C
Cuts at: 0 18 630
Size: 18 612
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 593 630
Size: 338 255 37
Fragments arranged by size:
338 255 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 121 151 155 630
Size: 117 4 30 4 475
Fragments arranged by size:
475 117 30 4 4
BclI T'GATC_A
Cuts at: 0 431 630
Size: 431 199
BglI GCCn_nnn'nGGC
Cuts at: 0 119 260 630
Size: 119 141 370
Fragments arranged by size:
370 141 119
BglII A'GATC_T
Cuts at: 0 414 630
Size: 414 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 515 525 630
Size: 415 100 10 105
Fragments arranged by size:
415 105 100 10
Bpu10I CC'TnA_GC
Cuts at: 0 488 630
Size: 488 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 630
Size: 268 362
BsaHI Gr'CG_yC
Cuts at: 0 15 40 445 630
Size: 15 25 405 185
Fragments arranged by size:
405 185 25 15
BsaWI w'CCGG_w
Cuts at: 0 616 630
Size: 616 14
BseYI C'CCAG_C
Cuts at: 0 46 630
Size: 46 584
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 630
Size: 132 498
BsiEI CG_ry'CG
Cuts at: 0 88 114 630
Size: 88 26 516
Fragments arranged by size:
516 88 26
BsiHKAI G_wGCw'C
Cuts at: 0 569 630
Size: 569 61
Bsp1286I G_dGCh'C
Cuts at: 0 569 630
Size: 569 61
BspEI T'CCGG_A
Cuts at: 0 616 630
Size: 616 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 630
Size: 154 476
BsrFI r'CCGG_y
Cuts at: 0 28 87 630
Size: 28 59 543
Fragments arranged by size:
543 59 28
BstBI TT'CG_AA
Cuts at: 0 169 630
Size: 169 461
BstYI r'GATC_y
Cuts at: 0 414 630
Size: 414 216
BtgI C'CryG_G
Cuts at: 0 82 117 630
Size: 82 35 513
Fragments arranged by size:
513 82 35
ClaI AT'CG_AT
Cuts at: 0 137 630
Size: 137 493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 390 425 630
Size: 390 35 205
Fragments arranged by size:
390 205 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 370 630
Size: 370 260
EaeI y'GGCC_r
Cuts at: 0 85 111 557 630
Size: 85 26 446 73
Fragments arranged by size:
446 85 73 26
EagI C'GGCC_G
Cuts at: 0 85 111 630
Size: 85 26 519
Fragments arranged by size:
519 85 26
EcoICRI GAG'CTC
Cuts at: 0 567 630
Size: 567 63
EcoO109I rG'GnC_Cy
Cuts at: 0 78 630
Size: 78 552
FspI TGC'GCA
Cuts at: 0 127 630
Size: 127 503
HaeII r_GCGC'y
Cuts at: 0 18 630
Size: 18 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 630
Size: 523 32 75
Fragments arranged by size:
523 75 32
KasI G'GCGC_C
Cuts at: 0 14 630
Size: 14 616
MspA1I CmG'CkG
Cuts at: 0 84 630
Size: 84 546
NaeI GCC'GGC
Cuts at: 0 30 89 630
Size: 30 59 541
Fragments arranged by size:
541 59 30
NarI GG'CG_CC
Cuts at: 0 15 630
Size: 15 615
NgoMIV G'CCGG_C
Cuts at: 0 28 87 630
Size: 28 59 543
Fragments arranged by size:
543 59 28
NotI GC'GGCC_GC
Cuts at: 0 111 630
Size: 111 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 481 630
Size: 481 149
PpuMI rG'GwC_Cy
Cuts at: 0 78 630
Size: 78 552
SacI G_AGCT'C
Cuts at: 0 569 630
Size: 569 61
SacII CC_GC'GG
Cuts at: 0 85 630
Size: 85 545
SanDI GG'GwC_CC
Cuts at: 0 78 630
Size: 78 552
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 119 630
Size: 119 511
SfoI GGC'GCC
Cuts at: 0 16 630
Size: 16 614
SmaI CCC'GGG
Cuts at: 0 76 630
Size: 76 554
StyI C'CwwG_G
Cuts at: 0 605 630
Size: 605 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 60 272 397 507 630
Size: 60 212 125 110 123
Fragments arranged by size:
212 125 123 110 60
TatI w'GTAC_w
Cuts at: 0 521 630
Size: 521 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 630
Size: 413 32 185
Fragments arranged by size:
413 185 32
XbaI T'CTAG_A
Cuts at: 0 267 630
Size: 267 363
XmaI C'CCGG_G
Cuts at: 0 74 630
Size: 74 556
Enzymes that do cut:
AflIII AhdI ApoI AvaI BanI BanII BbeI BbsI
BcgI BclI BglI BglII BmrI Bpu10I BsaI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BstBI BstYI BtgI ClaI CspCI DrdI EaeI
EagI EcoICRI EcoO109I FspI HaeII Hin4I KasI MspA1I
NaeI NarI NgoMIV NotI PflMI PpuMI SacI SacII
SanDI SfiI SfoI SmaI StyI TaqII TatI TstI
XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI
BlpI Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI DraI DraIII EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI
XmnI ZraI