Restriction map for pF1KE1875
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsiWI       
BsmI        BspDI       BspHI       BspLU11I    BssHII      
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       ClaI        DraI        DrdI        Eam1105I    
EarI        Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        XbaI        XhoI        
XhoII       XmnI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI         1158  GTATAC               GT^[AC][GT]AC
AccIII        230  TCCGGA               T^CCGGA
AlwI         1020  GATCC                GGATC(4/5)
AocI          391  CCTGAGG              CC^T[ACGT]AGG
AspI          511  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          285  CCCGGG               C^[CT]CG[GA]G
BalI          533  TGGCCA               TGG^CCA
BanI          265  GGCGCC               G^G[CT][GA]CC
BbeI          265  GGCGCC               GGCGC^C
BglI          284  GCCCGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          673  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      673  GCTAAGC              GC^T[ACGT]AGC
BseAI         230  TCCGGA               T^CCGGA
BsgI           94  CTGCAC               GTGCAG(16/14)
BsmBI         516  CGTCTC               CGTCTC(1/5)
BsoBI         285  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1140  TGTACA               T^GTACA
BspEI         230  TCCGGA               T^CCGGA
BspMI         724  ACCTGC               ACCTGC(4/8)
BsrDI         814  GCAATG               GCAATG(2/0)
BsrFI         557  ACCGGC               [GA]^CCGG[CT]
BsrGI        1140  TGTACA               T^GTACA
Bst1107I     1158  GTATAC               GTA^TAC
BstF5I       1137  GGATG                GGATG(2/0)
BstZ17I      1158  GTATAC               GTA^TAC
Bsu36I        391  CCTGAGG              CC^T[ACGT]AGG
CelII         673  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        557  ACCGGC               [GA]^CCGG[CT]
CpoI          232  CGGACCG              CG^G[AT]CCG
DpnII        1020  GATC                 ^GATC
DraIII        526  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          796  CCACGG               C^C[GA][CT]GG
Eco81I        391  CCTGAGG              CC^T[ACGT]AGG
EcoNI         256  CCTCCTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        99  CCTTGG               C^C[AT][AT]GG
FokI         1137  GGATG                GGATG(9/13)
KasI          265  GGCGCC               G^GCGCC
MaeII         743  ACGT                 A^CGT
MboI         1020  GATC                 ^GATC
MluNI         533  TGGCCA               TGG^CCA
MroI          230  TCCGGA               T^CCGGA
MscI          533  TGGCCA               TGG^CCA
NarI          265  GGCGCC               GG^CGCC
NdeII        1020  GATC                 ^GATC
NheI          331  GCTAGC               G^CTAGC
NotI          491  GCGGCCGC             GC^GGCCGC
PflFI         511  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         342  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         616  CAGCTG               CAG^CTG
RsrII         232  CGGACCG              CG^G[AT]CCG
Sau3AI       1020  GATC                 ^GATC
ScaI          575  AGTACT               AGT^ACT
SexAI        1127  ACCAGGT              A^CC[AT]GGT
SfoI          265  GGCGCC               GGC^GCC
SmaI          285  CCCGGG               CCC^GGG
SspBI        1140  TGTACA               T^GTACA
StyI           99  CCTTGG               C^C[AT][AT]GG
TaqI          190  TCGA                 T^CGA
Tth111I       511  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       190  TCGA                 T^CGA
XcmI          299  CCAGCCCAGTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         285  CCCGGG               C^CCGGG
XmaI          285  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          103  GGCGTC               G[GA]^CG[CT]C
              265  GGCGCC              
ApaI          242  GGGCCC               GGGCC^C
              414  GGGCCC              
BfaI          262  CTAG                 C^TAG
              332  CTAG                
BpmI          297  CTCCAG               CTGGAG(16/14)
              451  CTGGAG              
BsaHI         103  GGCGTC               G[GA]^CG[CT]C
              265  GGCGCC              
BsaWI         230  TCCGGA               [AT]^CCGG[AT]
              235  ACCGGA              
BsiEI         247  CGGCCG               CG[GA][CT]^CG
              492  CGGCCG              
BsrBI          16  CCGCTC               CCGCTC(-3/-3)
               24  GAGCGG              
BssSI          76  CTCGTG               CACGAG(-5/-1)
              457  CTCGTG              
DraII         241  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              414  GGGCCCC             
EagI          247  CGGCCG               C^GGCCG
              492  CGGCCG              
EclXI         247  CGGCCG               C^GGCCG
              492  CGGCCG              
Eco52I        247  CGGCCG               C^GGCCG
              492  CGGCCG              
EcoO109I      241  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              414  GGGCCCC             
Hin1I         103  GGCGTC               G[GA]^CG[CT]C
              265  GGCGCC              
HphI          107  TCACC                GGTGA(8/7)
             1121  TCACC               
MaeI          262  CTAG                 C^TAG
              332  CTAG                
MaeIII        106  GTCAC                ^GT[ACGT]AC
              740  GTGAC               
MboII         483  GAAGA                GAAGA(8/7)
              937  GAAGA               
MseI          434  TTAA                 T^TAA
              953  TTAA                
MslI          438  CATGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              753  CATTCCAATG          
NspI          437  ACATGC               [GA]CATG^[CT]
              974  GCATGT              
PflMI         612  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1128  CCAGGTGCTGG         
PstI          178  CTGCAG               CTGCA^G
              604  CTGCAG              
SacI          226  GAGCTC               GAGCT^C
              454  GAGCTC              
SfaNI          39  GATGC                GCATC(5/9)
              629  GCATC               
SmlI          571  CTCAAG               C^T[CT][GA]AG
             1029  CTCAAG              
TfiI          313  GATTC                G^A[AT]TC
              830  GATTC               
Tru9I         434  TTAA                 T^TAA
              953  TTAA                
Tsp45I        106  GTCAC                ^GT[GC]AC
              740  GTGAC               
Tsp509I       916  AATT                 ^AATT
             1074  AATT                
Van91I        612  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1128  CCAGGTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        EaeI        EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PleI        
RsaI        Sau96I      ScrFI       SfcI        TseI        
TspRI       
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