Restriction map for pF1KE1598
 (Linear) (Six-base) MAPSORT of: KIEE1598.seq  Check: 5562  from: 1  to: 4296



KIEE1598.orf

 With 182 enzymes: SgfI * 

                        August 16, 2010 15:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     397    4296 
   Size:        397    3899
 
AccI GT'mk_AC

Cuts at:      0     904    1916    4296 
   Size:        904    1012    2380

  Fragments arranged by size: 

                2380    1012     904
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2224    3635    4154    4296 
   Size:       2224    1411     519     142

  Fragments arranged by size: 

                2224    1411     519     142
 
AflII C'TTAA_G

Cuts at:      0    1345    1426    4296 
   Size:       1345      81    2870

  Fragments arranged by size: 

                2870    1345      81
 
AflIII A'CryG_T

Cuts at:      0    4031    4296 
   Size:       4031     265
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     725    3515    3859    4296 
   Size:        725    2790     344     437

  Fragments arranged by size: 

                2790     725     437     344
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1187    1219    4296 
   Size:       1187      32    3077

  Fragments arranged by size: 

                3077    1187      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     567     630     643    2247    4296 
   Size:        567      63      13    1604    2049

  Fragments arranged by size: 

                2049    1604     567      63      13
 
ApoI r'AATT_y

Cuts at:      0     142     468     604     675    1208    2284    2441    2926 
   Size:        142     326     136      71     533    1076     157     485

Cuts at:   2926    3106    4296 
   Size:        180    1190

  Fragments arranged by size: 

                1190    1076     533     485     326     180     157     142
                 136      71
 
AseI AT'TA_AT

Cuts at:      0    2483    4261    4296 
   Size:       2483    1778      35

  Fragments arranged by size: 

                2483    1778      35
 
BamHI G'GATC_C

Cuts at:      0     151    4296 
   Size:        151    4145
 
BanI G'GyrC_C

Cuts at:      0    2630    3253    4296 
   Size:       2630     623    1043

  Fragments arranged by size: 

                2630    1043     623
 
BanII G_rGCy'C

Cuts at:      0    2065    2607    4296 
   Size:       2065     542    1689

  Fragments arranged by size: 

                2065    1689     542
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1142    1498    1814    2141    3733    4296 
   Size:       1142     356     316     327    1592     563

  Fragments arranged by size: 

                1592    1142     563     356     327     316
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1757    2853    4296 
   Size:       1757    1096    1443

  Fragments arranged by size: 

                1757    1443    1096
 
BclI T'GATC_A

Cuts at:      0    1053    3065    4296 
   Size:       1053    2012    1231

  Fragments arranged by size: 

                2012    1231    1053
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3634    4296 
   Size:       3634     662
 
BglII A'GATC_T

Cuts at:      0    4139    4296 
   Size:       4139     157
 
BlpI GC'TnA_GC

Cuts at:      0     794    4296 
   Size:        794    3502
 
Bme1580I G_kGCm'C

Cuts at:      0    3256    4296 
   Size:       3256    1040
 
BmgBI CAC'GTC

Cuts at:      0    2595    4296 
   Size:       2595    1701
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     983    2289    4296 
   Size:        983    1306    2007

  Fragments arranged by size: 

                2007    1306     983
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2183    4296 
   Size:       2183    2113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543    1910    1942    3911    4296 
   Size:        543    1367      32    1969     385

  Fragments arranged by size: 

                1969    1367     543     385      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1603    1987    3698    4296 
   Size:       1603     384    1711     598

  Fragments arranged by size: 

                1711    1603     598     384
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     511    3564    4296 
   Size:        511    3053     732

  Fragments arranged by size: 

                3053     732     511
 
BsaAI yAC'GTr

Cuts at:      0     445    1920    2650    4034    4099    4296 
   Size:        445    1475     730    1384      65     197

  Fragments arranged by size: 

                1475    1384     730     445     197      65
 
BsaBI GATnn'nnATC

Cuts at:      0    2990    4296 
   Size:       2990    1306
 
BsaWI w'CCGG_w

Cuts at:      0    1907    4296 
   Size:       1907    2389
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1067    1097    1187    1217    4296 
   Size:       1067      30      90      30    3079

  Fragments arranged by size: 

                3079    1067      90      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     900    4296 
   Size:        900    3396
 
BseYI C'CCAG_C

Cuts at:      0     931    1842    3522    4296 
   Size:        931     911    1680     774

  Fragments arranged by size: 

                1680     931     911     774
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11      82    4296 
   Size:         11      71    4214

  Fragments arranged by size: 

                4214      71      11
 
BsiHKAI G_wGCw'C

Cuts at:      0    2523    4296 
   Size:       2523    1773
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4206    4296 
   Size:       4206      90
 
Bsp1286I G_dGCh'C

Cuts at:      0    2065    2523    2607    3256    4296 
   Size:       2065     458      84     649    1040

  Fragments arranged by size: 

                2065    1040     649     458      84
 
BspHI T'CATG_A

Cuts at:      0    3851    4296 
   Size:       3851     445
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     397    2807    4296 
   Size:        397    2410    1489

  Fragments arranged by size: 

                2410    1489     397
 
BsrDI GCAATG_nn'

Cuts at:      0    1991    2832    4296 
   Size:       1991     841    1464

  Fragments arranged by size: 

                1991    1464     841
 
BsrGI T'GTAC_A

Cuts at:      0     511    4296 
   Size:        511    3785
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2107    4296 
   Size:       2107    2189
 
BstBI TT'CG_AA

Cuts at:      0    3235    4296 
   Size:       3235    1061
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1658    3510    4296 
   Size:       1658    1852     786

  Fragments arranged by size: 

                1852    1658     786
 
BstYI r'GATC_y

Cuts at:      0     151     786    2244    2962    4058    4139    4296 
   Size:        151     635    1458     718    1096      81     157

  Fragments arranged by size: 

                1458    1096     718     635     157     151      81
 
BstZ17I GTA'TAC

Cuts at:      0    1917    4296 
   Size:       1917    2379
 
Bsu36I CC'TnA_GG

Cuts at:      0    2525    4296 
   Size:       2525    1771
 
BtgI C'CryG_G

Cuts at:      0     832    2843    4296 
   Size:        832    2011    1453

  Fragments arranged by size: 

                2011    1453     832
 
BtsI GCAGTG_nn'

Cuts at:      0    2077    4296 
   Size:       2077    2219
 
DraI TTT'AAA

Cuts at:      0     944    1647    4296 
   Size:        944     703    2649

  Fragments arranged by size: 

                2649     944     703
 
EaeI y'GGCC_r

Cuts at:      0     253    1585    1632    3560    3776    4296 
   Size:        253    1332      47    1928     216     520

  Fragments arranged by size: 

                1928    1332     520     253     216      47
 
EarI CTCTTCn'nnn_

Cuts at:      0     103    1595    1686    3814    4130    4296 
   Size:        103    1492      91    2128     316     166

  Fragments arranged by size: 

                2128    1492     316     166     103      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543    1910    1942    2224    3635    3911    4154    4296 
   Size:        543    1367      32     282    1411     276     243     142

  Fragments arranged by size: 

                1411    1367     543     282     276     243     142      32
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1041    4296 
   Size:       1041    3255
 
EcoRI G'AATT_C

Cuts at:      0     142    4296 
   Size:        142    4154
 
EcoRV GAT'ATC

Cuts at:      0     456    4296 
   Size:        456    3840
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1102    1255    1644    3330    4296 
   Size:       1102     153     389    1686     966

  Fragments arranged by size: 

                1686    1102     966     389     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1258    1290    2521    2553    2720    2752    2848    2880 
   Size:       1258      32    1231      32     167      32      96      32

Cuts at:   2880    3292    3324    3831    3863    4180    4212    4296 
   Size:        412      32     507      32     317      32      84

  Fragments arranged by size: 

                1258    1231     507     412     317     167      96      84
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     482     761    3975    4107    4296 
   Size:        482     279    3214     132     189

  Fragments arranged by size: 

                3214     482     279     189     132
 
HindIII A'AGCT_T

Cuts at:      0    2893    3407    4296 
   Size:       2893     514     889

  Fragments arranged by size: 

                2893     889     514
 
MfeI C'AATT_G

Cuts at:      0     594    3857    4052    4296 
   Size:        594    3263     195     244

  Fragments arranged by size: 

                3263     594     244     195
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1859    2320    3626    4296 
   Size:       1859     461    1306     670

  Fragments arranged by size: 

                1859    1306     670     461
 
MscI TGG'CCA

Cuts at:      0     255    1587    1634    3562    3778    4296 
   Size:        255    1332      47    1928     216     518

  Fragments arranged by size: 

                1928    1332     518     255     216      47
 
MslI CAynn'nnrTG

Cuts at:      0    1996    2516    4296 
   Size:       1996     520    1780

  Fragments arranged by size: 

                1996    1780     520
 
MspA1I CmG'CkG

Cuts at:      0      36     587    3063    4082    4296 
   Size:         36     551    2476    1019     214

  Fragments arranged by size: 

                2476    1019     551     214      36
 
NcoI C'CATG_G

Cuts at:      0    2843    4296 
   Size:       2843    1453
 
NspI r_CATG'y

Cuts at:      0     101    3502    3867    4296 
   Size:        101    3401     365     429

  Fragments arranged by size: 

                3401     429     365     101
 
PasI CC'CwG_GG

Cuts at:      0     363    4296 
   Size:        363    3933
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1212    4296 
   Size:       1212    3084
 
PfoI T'CCnGG_A

Cuts at:      0    2129    4296 
   Size:       2129    2167
 
PmlI CAC'GTG

Cuts at:      0    4099    4296 
   Size:       4099     197
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1187    1219    4296 
   Size:       1187      32    3077

  Fragments arranged by size: 

                3077    1187      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1041    4296 
   Size:       1041    3255
 
PshAI GACnn'nnGTC

Cuts at:      0    2876    4296 
   Size:       2876    1420
 
PsiI TTA'TAA

Cuts at:      0    1256    3272    3335    4296 
   Size:       1256    2016      63     961

  Fragments arranged by size: 

                2016    1256     961      63
 
PstI C_TGCA'G

Cuts at:      0    2252    2613    4203    4296 
   Size:       2252     361    1590      93

  Fragments arranged by size: 

                2252    1590     361      93
 
PvuII CAG'CTG

Cuts at:      0    3063    4296 
   Size:       3063    1233
 
SanDI GG'GwC_CC

Cuts at:      0    1041    4296 
   Size:       1041    3255
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1595    3814    4296 
   Size:       1595    2219     482

  Fragments arranged by size: 

                2219    1595     482
 
SfcI C'TryA_G

Cuts at:      0      80     588    1819    2248    2609    4199    4296 
   Size:         80     508    1231     429     361    1590      97

  Fragments arranged by size: 

                1590    1231     508     429     361      97      80
 
SmlI C'TyrA_G

Cuts at:      0    1345    1426    1618    1966    3713    4296 
   Size:       1345      81     192     348    1747     583

  Fragments arranged by size: 

                1747    1345     583     348     192      81
 
SnaBI TAC'GTA

Cuts at:      0    1920    2650    4296 
   Size:       1920     730    1646

  Fragments arranged by size: 

                1920    1646     730
 
SphI G_CATG'C

Cuts at:      0    3867    4296 
   Size:       3867     429
 
StuI AGG'CCT

Cuts at:      0    3920    4296 
   Size:       3920     376
 
StyI C'CwwG_G

Cuts at:      0    1854    2777    2843    4296 
   Size:       1854     923      66    1453

  Fragments arranged by size: 

                1854    1453     923      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1608    4296 
   Size:       1608    2688
 
TatI w'GTAC_w

Cuts at:      0     511     981    1083    1625    2179    2763    3295    3703 
   Size:        511     470     102     542     554     584     532     408

Cuts at:   3703    4296 
   Size:        593

  Fragments arranged by size: 

                 593     584     554     542     532     511     470     408
                 102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     143     175     328     360    1082    1114    1567    1599 
   Size:        143      32     153      32     722      32     453      32

Cuts at:   1599    3591    3623    4296 
   Size:       1992      32     673

  Fragments arranged by size: 

                1992     722     673     453     153     143      32      32
                  32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2317    4296 
   Size:       2317    1979
 
XmnI GAAnn'nnTTC

Cuts at:      0    1877    4296 
   Size:       1877    2419
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI
     ApoI     AseI    BamHI     BanI    BanII     BbsI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI     PsiI     PstI    PvuII    SanDI     SapI     SfcI     SmlI
    SnaBI     SphI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BfrBI     BmtI   Bpu10I    BsaHI    BsiEI    BsiWI     BsmI
    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstEII    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SrfI     SspI     SwaI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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