Restriction map for pF1KE1794
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbsI        BbvCI       BclI        
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MluI        MluNI       MroI        MscI        MvnI        
MwoI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
PstI        PvuI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          259  GTAGAC               GT^[AC][GT]AC
AciI           84  GCGG                 CCGC(-3/-1)
AclI          421  AACGTT               AA^CGTT
AcsI          110  AAATTT               [GA]^AATT[CT]
Alw44I        782  GTGCAC               G^TGCAC
ApaI          576  GGGCCC               GGGCC^C
ApaLI         782  GTGCAC               G^TGCAC
ApoI          110  AAATTT               [GA]^AATT[CT]
AspHI         782  GTGCAC               G[AT]GC[AT]^C
AvaII          30  GGACC                G^G[AT]CC
BbrPI         638  CACGTG               CAC^GTG
BcgI          135  GCAAGAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         441  AGATCT               A^GATCT
BsaAI         638  CACGTG               [CT]AC^GT[GA]
BsaI          697  GGTCTC               GGTCTC(1/5)
BsaWI         225  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       782  GTGCAC               G[AT]GC[AT]^C
BsmI          414  GAATGC               GAATGC(1/-1)
BssSI         713  CTCGTG               CACGAG(-5/-1)
BstEII        768  GGTTACC              G^GT[ACGT]ACC
BstPI         768  GGTTACC              G^GT[ACGT]ACC
Cac8I         646  GCTGGC               GC[ACGT]^[ACGT]GC
DraI          843  TTTAAA               TTT^AAA
DraII         575  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI           31  GACCAGTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          498  CCATGG               C^C[GA][CT]GG
EarI          293  CTCTTC               CTCTTC(1/4)
EcoO109I      575  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       768  GGTTACC              G^GT[ACGT]ACC
HaeII         873  AGCGCC               [GA]GCGC^[CT]
HapII         226  CCGG                 C^CGG
HgaI           87  GACGC                GACGC(5/10)
HpaII         226  CCGG                 C^CGG
Ksp632I       293  CTCTTC               CTCTTC(1/4)
MfeI          744  CAATTG               C^AATTG
MslI          688  CATGCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          226  CCGG                 C^CGG
MunI          744  CAATTG               C^AATTG
NcoI          498  CCATGG               C^CATGG
PflMI         419  CCAACGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          291  GACTC                GAGTC(4/5)
PmaCI         638  CACGTG               CAC^GTG
PmlI          638  CACGTG               CAC^GTG
Psp1406I      421  AACGTT               AA^CGTT
TaqI           41  TCGA                 T^CGA
TfiI          124  GAATC                G^A[AT]TC
TthHB8I        41  TCGA                 T^CGA
Van91I        419  CCAACGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          518  CCATAGACACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

BanII         542  GAGCCC               G[GA]GC[CT]^C
              576  GGGCCC              
BciVI         228  GGATAC               GTATCC(6/5)
              739  GGATAC              
BsmAI         283  GAGAC                GTCTC(1/5)
              698  GTCTC               
BspHI         186  TCATGA               T^CATGA
              753  TCATGA              
BssKI         546  CCTGG                ^CC[ACGT]GG
              813  CCAGG               
BstF5I        515  CATCC                GGATG(2/0)
              622  GGATG               
BstNI         546  CCTGG                CC^[AT]GG
              813  CCAGG               
BstYI         441  AGATCT               [GA]^GATC[CT]
              603  AGATCC              
CfoI          874  GCGC                 GCG^C
              906  GCGC                
DraIII        635  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              638  CACGTGGTG           
EcoRII        546  CCTGG                ^CC[AT]GG
              813  CCAGG               
EcoT14I        20  CCAAGG               C^C[AT][AT]GG
              498  CCATGG              
FokI          515  CATCC                GGATG(9/13)
              622  GGATG               
HaeIII        505  GGCC                 GG^CC
              577  GGCC                
HhaI          874  GCGC                 GCG^C
              906  GCGC                
HinP1I        874  GCGC                 G^CGC
              906  GCGC                
HinfI         124  GAATC                G^A[ACGT]TC
              291  GACTC               
MaeII         422  ACGT                 A^CGT
              639  ACGT                
MboII         294  TCTTC                GAAGA(8/7)
              456  TCTTC               
MflI          441  AGATCT               [GA]^GATC[CT]
              603  AGATCC              
MspA1I         46  CAGCTG               C[AC]G^C[GT]G
               64  CAGCTG              
MvaI          546  CCTGG                CC^[AT]GG
              813  CCAGG               
NlaIV         576  GGGCCC               GG[ACGT]^[ACGT]CC
              809  GGAACC              
PvuII          46  CAGCTG               CAG^CTG
               64  CAGCTG              
RcaI          186  TCATGA               T^CATGA
              753  TCATGA              
ScrFI         546  CCTGG                CC^[ACGT]GG
              813  CCAGG               
StyI           20  CCAAGG               C^C[AT][AT]GG
              498  CCATGG              
Tsp45I        251  GTGAC                ^GT[GC]AC
              289  GTGAC               
XhoII         441  AGATCT               [GA]^GATC[CT]
              603  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        AlwI        BbvI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BstXI       
Cfr13I      DpnII       Fnu4HI      HphI        ItaI        
MaeIII      MboI        MnlI        MseI        NdeII       
NlaIII      Sau3AI      Sau96I      SspI        Tru9I       
TseI        Tsp509I     TspRI       
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