Restriction map for pF1KE1794
 (Linear) (Six-base) MAPSORT of: KIEE1794.seq  Check: 1811  from: 1  to: 927



KIEE1794.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     260     927 
   Size:        260     667
 
AclI AA'CG_TT

Cuts at:      0     422     927 
   Size:        422     505
 
ApaI G_GGCC'C

Cuts at:      0     580     927 
   Size:        580     347
 
ApaLI G'TGCA_C

Cuts at:      0     782     927 
   Size:        782     145
 
ApoI r'AATT_y

Cuts at:      0     110     927 
   Size:        110     817
 
BanII G_rGCy'C

Cuts at:      0     546     580     927 
   Size:        546      34     347

  Fragments arranged by size: 

                 546     347      34
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     124     158     927 
   Size:        124      34     769

  Fragments arranged by size: 

                 769     124      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     222     733     927 
   Size:        222     511     194

  Fragments arranged by size: 

                 511     222     194
 
BglII A'GATC_T

Cuts at:      0     441     927 
   Size:        441     486
 
Bme1580I G_kGCm'C

Cuts at:      0     580     720     786     927 
   Size:        580     140      66     141

  Fragments arranged by size: 

                 580     141     140      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     703     927 
   Size:        703     224
 
BsaAI yAC'GTr

Cuts at:      0     640     927 
   Size:        640     287
 
BsaWI w'CCGG_w

Cuts at:      0     225     927 
   Size:        225     702
 
BseYI C'CCAG_C

Cuts at:      0     870     927 
   Size:        870      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     786     927 
   Size:        786     141
 
BsmI GAATG_Cn'

Cuts at:      0     420     927 
   Size:        420     507
 
Bsp1286I G_dGCh'C

Cuts at:      0     546     580     720     786     927 
   Size:        546      34     140      66     141

  Fragments arranged by size: 

                 546     141     140      66      34
 
BspHI T'CATG_A

Cuts at:      0     186     753     927 
   Size:        186     567     174

  Fragments arranged by size: 

                 567     186     174
 
BssSI C'ACGA_G

Cuts at:      0     713     927 
   Size:        713     214
 
BstEII G'GTnAC_C

Cuts at:      0     768     927 
   Size:        768     159
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      27     379     694     927 
   Size:         27     352     315     233

  Fragments arranged by size: 

                 352     315     233      27
 
BstYI r'GATC_y

Cuts at:      0     441     603     927 
   Size:        441     162     324

  Fragments arranged by size: 

                 441     324     162
 
BtgI C'CryG_G

Cuts at:      0     498     927 
   Size:        498     429
 
DraI TTT'AAA

Cuts at:      0     845     927 
   Size:        845      82
 
DraIII CAC_nnn'GTG

Cuts at:      0     640     643     927 
   Size:        640       3     284

  Fragments arranged by size: 

                 640     284       3
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      37     927 
   Size:         37     890
 
EarI CTCTTCn'nnn_

Cuts at:      0     299     927 
   Size:        299     628
 
EcoO109I rG'GnC_Cy

Cuts at:      0     576     927 
   Size:        576     351
 
HaeII r_GCGC'y

Cuts at:      0     877     927 
   Size:        877      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     276     308     333     365     927 
   Size:        276      32      25      32     562

  Fragments arranged by size: 

                 562     276      32      32      25
 
MfeI C'AATT_G

Cuts at:      0     744     927 
   Size:        744     183
 
MslI CAynn'nnrTG

Cuts at:      0     692     927 
   Size:        692     235
 
MspA1I CmG'CkG

Cuts at:      0      48      66     927 
   Size:         48      18     861

  Fragments arranged by size: 

                 861      48      18
 
NcoI C'CATG_G

Cuts at:      0     498     927 
   Size:        498     429
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     425     927 
   Size:        425     502
 
PmlI CAC'GTG

Cuts at:      0     640     927 
   Size:        640     287
 
PspOMI G'GGCC_C

Cuts at:      0     576     927 
   Size:        576     351
 
PvuII CAG'CTG

Cuts at:      0      48      66     927 
   Size:         48      18     861

  Fragments arranged by size: 

                 861      48      18
 
SspI AAT'ATT

Cuts at:      0     338     433     850     927 
   Size:        338      95     417      77

  Fragments arranged by size: 

                 417     338      95      77
 
StyI C'CwwG_G

Cuts at:      0      20     498     927 
   Size:         20     478     429

  Fragments arranged by size: 

                 478     429      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238     801     927 
   Size:        238     563     126

  Fragments arranged by size: 

                 563     238     126
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     525     927 
   Size:        525     402
 

 Enzymes that do cut:

     AccI     AclI     ApaI    ApaLI     ApoI    BanII     BcgI    BciVI
    BglII Bme1580I     BsaI    BsaAI    BsaWI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BssSI   BstEII    BstXI    BstYI     BtgI     DraI
   DraIII     DrdI     EarI EcoO109I    HaeII    Hin4I     MfeI     MslI
   MspA1I     NcoI    PflMI     PmlI   PspOMI    PvuII     SspI     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com