Restriction map for pF1KE4367
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AgeI        AocI        ApaI        AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        DraI        
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII         135  CGCG                 CG^CG
AcsI          703  AAATTC               [GA]^AATT[CT]
AflII         112  CTTAAG               C^TTAAG
AhdI           14  GACAAAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI        37  AGCGCT               AGC^GCT
ApoI          703  AAATTC               [GA]^AATT[CT]
AspEI          14  GACAAAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           69  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        1101  GGGCTC               G[GA]GC[CT]^C
BbsI          124  GTCTTC               GAAGAC(2/6)
BfrI          112  CTTAAG               C^TTAAG
BpmI          278  CTGGAG               CTGGAG(16/14)
BpuAI         124  GTCTTC               GAAGAC(2/6)
BsiEI          26  CGACCG               CG[GA][CT]^CG
Bsp1407I      352  TGTACA               T^GTACA
BsrDI         318  CATTGC               GCAATG(2/0)
BsrFI         147  GCCGGT               [GA]^CCGG[CT]
BsrGI         352  TGTACA               T^GTACA
BssSI         797  CACGAG               CACGAG(-5/-1)
BstAPI       1135  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         135  CGCG                 CG^CG
Cfr10I        147  GCCGGT               [GA]^CCGG[CT]
CpoI          149  CGGTCCG              CG^G[AT]CCG
DraII         747  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        408  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          918  GACTGTAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           88  CCATGG               C^C[GA][CT]GG
Eam1105I       14  GACAAAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       37  AGCGCT               AGC^GCT
EcoNI         231  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      747  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FauI          824  CCCGC                CCCGC(4/6)
HaeIII        207  GGCC                 GG^CC
HincII        157  GTTGAC               GT[CT]^[GA]AC
HindII        157  GTTGAC               GT[CT]^[GA]AC
HindIII       432  AAGCTT               A^AGCTT
MseI          113  TTAA                 T^TAA
MslI          642  CATCATAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          135  CGCG                 CG^CG
NcoI           88  CCATGG               C^CATGG
NspI         1068  ACATGC               [GA]CATG^[CT]
PflFI          69  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         747  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         551  CAGCTG               CAG^CTG
RsrII         149  CGGTCCG              CG^G[AT]CCG
SfcI          920  CTGTAG               C^T[GA][CT]AG
SspBI         352  TGTACA               T^GTACA
TfiI          547  GAATC                G^A[AT]TC
Tru9I         113  TTAA                 T^TAA
Tth111I        69  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        469  ACGTGT               A^C[GA][CT]GT
              756  ACGTGT              
Alw44I        294  GTGCAC               G^TGCAC
              760  GTGCAC              
AlwI          108  GATCC                GGATC(4/5)
              256  GGATC               
ApaLI         294  GTGCAC               G^TGCAC
              760  GTGCAC              
Asp700I       699  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1078  GAAACAATTC          
AspHI         294  GTGCAC               G[AT]GC[AT]^C
              760  GTGCAC              
AvaI           33  CTCGAG               C^[CT]CG[GA]G
               41  CTCGAG              
BsaI          832  GAGACC               GGTCTC(1/5)
              867  GGTCTC              
BsiHKAI       294  GTGCAC               G[AT]GC[AT]^C
              760  GTGCAC              
BsoBI          33  CTCGAG               C^[CT]CG[GA]G
               41  CTCGAG              
BstXI         488  CCAGAGGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              599  CCACCATCCTGG        
BstYI         107  AGATCC               [GA]^GATC[CT]
              256  GGATCT              
EcoT14I        88  CCATGG               C^C[AT][AT]GG
              885  CCAAGG              
HaeII          37  AGCGCT               [GA]GCGC^[CT]
               50  GGCGCT              
MflI          107  AGATCC               [GA]^GATC[CT]
              256  GGATCT              
PaeR7I         33  CTCGAG               C^TCGAG
               41  CTCGAG              
PflMI          83  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1092  CCAACTGGTGG         
StyI           88  CCATGG               C^C[AT][AT]GG
              885  CCAAGG              
TaqI           34  TCGA                 T^CGA
               42  TCGA                
TthHB8I        34  TCGA                 T^CGA
               42  TCGA                
Van91I         83  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1092  CCAACTGGTGG         
XhoI           33  CTCGAG               C^TCGAG
               41  CTCGAG              
XhoII         107  AGATCC               [GA]^GATC[CT]
              256  GGATCT              
XmnI          699  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1078  GAAACAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        TseI        
Tsp45I      Tsp509I     TspRI       
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