Restriction map for pF1KB5966
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BciVI       BfrI        BglII       BlnI        BsaBI       
BsaWI       BseAI       BsgI        BsiWI       BsoBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoT22I     FseI        FspI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         1453  GTCTAC               GT^[AC][GT]AC
AccII          99  CGCG                 CG^CG
AlwI         1325  GGATC                GGATC(4/5)
BanI          222  GGTGCC               G^G[CT][GA]CC
BbsI          121  GTCTTC               GAAGAC(2/6)
BcgI         1070  CGAGCTCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1178  TGATCA               T^GATCA
BlpI         1363  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1363  GCTGAGC              GC^T[ACGT]AGC
BpuAI         121  GTCTTC               GAAGAC(2/6)
BsaI         1570  GAGACC               GGTCTC(1/5)
BseRI        1022  CTCCTC               GAGGAG(10/8)
BsiEI        1194  CGACCG               CG[GA][CT]^CG
BsmBI         385  GAGACG               CGTCTC(1/5)
BsmFI         368  GGGAC                GGGAC(10/14)
BsmI          885  GAATGC               GAATGC(1/-1)
Bsp1407I     1055  TGTACA               T^GTACA
BspHI        1175  TCATGA               T^CATGA
BsrFI        1457  ACCGGC               [GA]^CCGG[CT]
BsrGI        1055  TGTACA               T^GTACA
BssSI        1068  CACGAG               CACGAG(-5/-1)
BstUI          99  CGCG                 CG^CG
CelII        1363  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1457  ACCGGC               [GA]^CCGG[CT]
DsaI           11  CCGTGG               C^C[GA][CT]GG
EarI          970  GAAGAG               CTCTTC(1/4)
EcoRI        1159  GAATTC               G^AATTC
EcoRV         695  GATATC               GAT^ATC
FbaI         1178  TGATCA               T^GATCA
HindIII      1559  AAGCTT               A^AGCTT
Ksp632I       970  GAAGAG               CTCTTC(1/4)
MvnI           99  CGCG                 CG^CG
NheI          753  GCTAGC               G^CTAGC
PpuMI         234  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1175  TCATGA               T^CATGA
SmlI         1477  CTTGAG               C^T[CT][GA]AG
SspBI        1055  TGTACA               T^GTACA
XcmI          592  CCAACCTATACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          679  GAATTT               [GA]^AATT[CT]
             1159  GAATTC              
AlwNI         233  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              955  CAGCGCCTG           
ApaI          228  GGGCCC               GGGCC^C
              357  GGGCCC              
ApoI          679  GAATTT               [GA]^AATT[CT]
             1159  GAATTC              
AspI           16  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1009  GACTTGGTC           
BalI          320  TGGCCA               TGG^CCA
              581  TGGCCA              
BfaI          754  CTAG                 C^TAG
             1648  CTAG                
BglI          365  GCCGGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1249  GCCTGTCTGGC         
BsaAI         109  TACGTG               [CT]AC^GT[GA]
              600  TACGTG              
BspMI         237  ACCTGC               ACCTGC(4/8)
             1114  GCAGGT              
BstXI           5  CCACCACCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              104  CCATCTACGTGG        
EcoT14I       627  CCAAGG               C^C[AT][AT]GG
             1216  CCTTGG              
HaeII         269  GGCGCT               [GA]GCGC^[CT]
              956  AGCGCC              
MaeI          754  CTAG                 C^TAG
             1648  CTAG                
MaeII         110  ACGT                 A^CGT
              601  ACGT                
MluNI         320  TGGCCA               TGG^CCA
              581  TGGCCA              
MscI          320  TGGCCA               TGG^CCA
              581  TGGCCA              
PflFI          16  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1009  GACTTGGTC           
PflMI         431  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              627  CCAAGGACTGG         
PstI          200  CTGCAG               CTGCA^G
              563  CTGCAG              
PvuII         566  CAGCTG               CAG^CTG
             1166  CAGCTG              
SacI         1071  GAGCTC               GAGCT^C
             1480  GAGCTC              
SexAI         419  ACCTGGT              A^CC[AT]GGT
             1272  ACCTGGT             
StyI          627  CCAAGG               C^C[AT][AT]GG
             1216  CCTTGG              
Tth111I        16  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1009  GACTTGGTC           
Van91I        431  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              627  CCAAGGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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