Restriction map for pF1KB6450
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BbeI        BbrPI       
BbvCI       BbvI        BcgI        BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        Fnu4HI      
FseI        FspI        Hin1I       HincII      HindII      
HpaI        ItaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       StuI        
StyI        SwaI        TseI        Tsp45I      Tth111I     
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI          997  CCGC                 CCGC(-3/-1)
AfaI          190  GTAC                 GT^AC
Aor51HI       693  AGCGCT               AGC^GCT
AseI          399  ATTAAT               AT^TAAT
AsnI          399  ATTAAT               AT^TAAT
Asp700I        97  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         244  GGACC                G^G[AT]CC
BamHI          51  GGATCC               G^GATCC
BanII         737  GAGCCC               G[GA]GC[CT]^C
BbsI          127  GAAGAC               GAAGAC(2/6)
BfaI          980  CTAG                 C^TAG
BglI          739  GCCCGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          782  CTCCAG               CTGGAG(16/14)
BpuAI         127  GAAGAC               GAAGAC(2/6)
BsmAI         856  GAGAC                GTCTC(1/5)
BsmFI         724  GTCCC                GGGAC(10/14)
BsrI          700  CCAGT                ACTGG(1/-1)
BstF5I        933  GGATG                GGATG(2/0)
BstXI         955  CCAGAAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          51  GGATCC               [GA]^GATC[CT]
Cac8I          66  GCATGC               GC[ACGT]^[ACGT]GC
DraIII        887  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      693  AGCGCT               AGC^GCT
EcoT22I       449  ATGCAT               ATGCA^T
FokI          933  GGATG                GGATG(9/13)
HaeII         693  AGCGCT               [GA]GCGC^[CT]
HapII         741  CCGG                 C^CGG
HgaI           70  GCGTC                GACGC(5/10)
HpaII         741  CCGG                 C^CGG
MaeI          980  CTAG                 C^TAG
MaeIII        682  GTAAC                ^GT[ACGT]AC
MflI           51  GGATCC               [GA]^GATC[CT]
MspI          741  CCGG                 C^CGG
NsiI          449  ATGCAT               ATGCA^T
NspI           66  GCATGC               [GA]CATG^[CT]
PaeR7I        734  CTCGAG               C^TCGAG
PflMI         640  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         399  ATTAAT               AT^TAAT
RsaI          190  GTAC                 GT^AC
ScaI          189  AGTACT               AGT^ACT
SfaNI         934  GATGC                GCATC(5/9)
SmaI          740  CCCGGG               CCC^GGG
SphI           66  GCATGC               GCATG^C
SspI           14  AATATT               AAT^ATT
TaqI          735  TCGA                 T^CGA
TfiI          195  GATTC                G^A[AT]TC
TthHB8I       735  TCGA                 T^CGA
Van91I        640  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          734  CTCGAG               C^TCGAG
XhoII          51  GGATCC               [GA]^GATC[CT]
XmaCI         740  CCCGGG               C^CCGGG
XmaI          740  CCCGGG               C^CCGGG
XmnI           97  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII          74  CGCG                 CG^CG
              876  CGCG                
AcsI          469  AAATTC               [GA]^AATT[CT]
              497  AAATTC              
ApoI          469  AAATTC               [GA]^AATT[CT]
              497  AAATTC              
BcnI          740  CCCGG                CC^[GC]GG
              741  CCGGG               
BsaAI         437  CACGTA               [CT]AC^GT[GA]
              515  TACGTG              
BstUI          74  CGCG                 CG^CG
              876  CGCG                
CfoI           75  GCGC                 GCG^C
              694  GCGC                
DsaI          615  CCGTGG               C^C[GA][CT]GG
              687  CCGTGG              
EcoNI         260  CCTTGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              646  CCTGGACGAGG         
HaeIII        613  GGCC                 GG^CC
              966  GGCC                
HhaI           75  GCGC                 GCG^C
              694  GCGC                
HinP1I         75  GCGC                 G^CGC
              694  GCGC                
HindIII       428  AAGCTT               A^AGCTT
              794  AAGCTT              
HphI          832  GGTGA                GGTGA(8/7)
              662  TCACC               
MaeII         438  ACGT                 A^CGT
              516  ACGT                
MslI          376  CACTTGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1030  CACCCCTATG          
MvnI           74  CGCG                 CG^CG
              876  CGCG                
NciI          740  CCCGG                CC^[GC]GG
              741  CCGGG               
PleI          547  GACTC                GAGTC(4/5)
              444  GAGTC               
PshAI         547  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              910  GACTACGGTC          
SfcI          271  CTACAG               C^T[GA][CT]AG
              710  CTGTAG              
SmlI          378  CTTGAG               C^T[CT][GA]AG
              734  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AluI        AlwI        AvaI        BanI        BciVI       
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsoBI       Bsp1286I    BssKI       BstNI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      HinfI       
Ksp632I     MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       Tru9I       Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com