Restriction map for pF1KE1218
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AhdI        Alw44I      
AlwI        AlwNI       AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BseAI       BseRI       BsgI        
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DpnII       DraIII      DrdI        Eam1105I    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HincII      
HindII      HindIII     HinfI       HpaI        KpnI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        NcoI        NdeI        NdeII       NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI           89  GTAC                 GT^AC
AgeI          295  ACCGGT               A^CCGGT
Aor51HI       448  AGCGCT               AGC^GCT
AspHI         124  GAGCTC               G[AT]GC[AT]^C
AvaII         109  GGTCC                G^G[AT]CC
BbeI          624  GGCGCC               GGCGC^C
BglI           73  GCCAAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          592  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      592  GCTAAGC              GC^T[ACGT]AGC
BsaI          247  GGTCTC               GGTCTC(1/5)
BsiEI         482  CGGCCG               CG[GA][CT]^CG
BsiHKAI       124  GAGCTC               G[AT]GC[AT]^C
BsiYI         519  CCCAAAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          519  CCCAAAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         110  GTCCC                GGGAC(10/14)
BsrBI          20  CCGCTC               CCGCTC(-3/-3)
BsrI          391  CCAGT                ACTGG(1/-1)
BstNI         258  CCTGG                CC^[AT]GG
CelII         592  GCTAAGC              GC^T[ACGT]AGC
DraI          313  TTTAAA               TTT^AAA
DraII          50  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          485  CCGCGG               C^C[GA][CT]GG
EagI          482  CGGCCG               C^GGCCG
EarI          444  GAAGAG               CTCTTC(1/4)
EclXI         482  CGGCCG               C^GGCCG
Eco47III      448  AGCGCT               AGC^GCT
Eco52I        482  CGGCCG               C^GGCCG
EcoO109I       50  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        258  CCTGG                ^CC[AT]GG
HgaI          103  GCGTC                GACGC(5/10)
KasI          624  GGCGCC               G^GCGCC
Ksp632I       444  GAAGAG               CTCTTC(1/4)
KspI          485  CCGCGG               CCGC^GG
MaeIII        496  GTAAC                ^GT[ACGT]AC
MslI          273  CACTCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          258  CCTGG                CC^[AT]GG
NarI          624  GGCGCC               GG^CGCC
PinAI         295  ACCGGT               A^CCGGT
RsaI           89  GTAC                 GT^AC
SacI          124  GAGCTC               GAGCT^C
SacII         485  CCGCGG               CCGC^GG
SapI          444  GAAGAGC              GCTCTTC(1/4)
SfoI          624  GGCGCC               GGC^GCC
SgrAI         294  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          250  CTCAAG               C^T[CT][GA]AG
TspRI         492  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          33  CGCG                 CG^CG
              486  CGCG                
AcyI          102  GGCGTC               G[GA]^CG[CT]C
              624  GGCGCC              
AluI          125  AGCT                 AG^CT
              552  AGCT                
BanI          292  GGCACC               G^G[CT][GA]CC
              624  GGCGCC              
BanII         124  GAGCTC               G[GA]GC[CT]^C
              516  GAGCCC              
BcnI          114  CCCGG                CC^[GC]GG
              338  CCGGG               
BsaHI         102  GGCGTC               G[GA]^CG[CT]C
              624  GGCGCC              
BsaWI         106  TCCGGT               [AT]^CCGG[AT]
              295  ACCGGT              
BsmAI           7  GAGAC                GTCTC(1/5)
              248  GTCTC               
BstUI          33  CGCG                 CG^CG
              486  CGCG                
BstXI         134  CCAAGGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              500  CCAAGAAAGTGG        
Cfr13I         51  GGCCC                G^G[ACGT]CC
              109  GGTCC               
FauI           25  CCCGC                CCCGC(4/6)
              367  GCGGG               
HaeII         448  AGCGCT               [GA]GCGC^[CT]
              624  GGCGCC              
Hin1I         102  GGCGTC               G[GA]^CG[CT]C
              624  GGCGCC              
HphI          261  GGTGA                GGTGA(8/7)
              131  TCACC               
MseI          314  TTAA                 T^TAA
              359  TTAA                
MspA1I         26  CCGCTG               C[AC]G^C[GT]G
              485  CCGCGG              
MvnI           33  CGCG                 CG^CG
              486  CGCG                
NaeI          205  GCCGGC               GCC^GGC
              480  GCCGGC              
NciI          114  CCCGG                CC^[GC]GG
              338  CCGGG               
NgoMIV        205  GCCGGC               G^CCGGC
              480  GCCGGC              
Sau96I         51  GGCCC                G^G[ACGT]CC
              109  GGTCC               
Tru9I         314  TTAA                 T^TAA
              359  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        BbvI        BmyI        BsaJI       Bsp1286I    
BsrFI       BssKI       Cac8I       CfoI        Cfr10I      
DdeI        EaeI        EcoT14I     Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboII       MnlI        MspI        MwoI        NlaIV       
ScrFI       StyI        TseI        
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