Restriction map for
pF1KE1218
(Circular) (Six-base) MAPSORT of: pF1KE1218.seq Check: 5244 from: 1 to: 3752
pF1KE1218 3752 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2009 2756 3024 1577
Size: 432 747 268 2305
Fragments arranged by size:
2305 747 432 268
AfeI AGC'GCT
Cuts at: 558 558
Size: 3752
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 403 2140 403
Size: 1737 2015
AleI CACnn'nnGTG
Cuts at: 385 385
Size: 3752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 772 772
Size: 3752
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 327 360 327
Size: 33 3719
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 400 732 768 1182 1430 1465 400
Size: 332 36 414 248 35 2687
Fragments arranged by size:
2687 414 332 248 36 35
BanII G_rGCy'C
Cuts at: 236 628 766 1796 236
Size: 392 138 1030 2192
Fragments arranged by size:
2192 1030 392 138
BbeI G_GCGC'C
Cuts at: 736 1186 1434 736
Size: 450 248 3054
Fragments arranged by size:
3054 450 248
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 187 187
Size: 3752
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 701 864 701
Size: 163 3589
Bme1580I G_kGCm'C
Cuts at: 382 1377 1470 2526 382
Size: 995 93 1056 1608
Fragments arranged by size:
1608 1056 995 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
Bpu10I CC'TnA_GC
Cuts at: 677 677
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 928 1807 2299 2597 343
Size: 585 879 492 298 1498
Fragments arranged by size:
1498 879 585 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 3456 361
Size: 3095 657
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 211 733 1183 1431 2133 3629 211
Size: 522 450 248 702 1496 334
Fragments arranged by size:
1496 702 522 450 334 248
BsaWI w'CCGG_w
Cuts at: 214 403 941 1462 2140 2414 2561 2972 214
Size: 189 538 521 678 274 147 411 994
Fragments arranged by size:
994 678 538 521 411 274 189 147
BseYI C'CCAG_C
Cuts at: 190 1177 1935 2512 3008 190
Size: 987 758 577 496 934
Fragments arranged by size:
987 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 593 1294 1340 2548 104
Size: 489 701 46 1208 1308
Fragments arranged by size:
1308 1208 701 489 46
BsiHKAI G_wGCw'C
Cuts at: 236 766 1544 1734 2526 236
Size: 530 778 190 792 1462
Fragments arranged by size:
1462 792 778 530 190
Bsp1286I G_dGCh'C
Cuts at: 236 382 628 766 1377 1470 1544 1734 1796
Size: 146 246 138 611 93 74 190 62
Cuts at: 1796 2526 236
Size: 730 1462
Fragments arranged by size:
1462 730 611 246 190 146 138 93
74 62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 130 2044 2098 3582 3747 50
Size: 80 1914 54 1484 165 55
Fragments arranged by size:
1914 1484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 313 403 588 1750 1931 2140 313
Size: 90 185 1162 181 209 1925
Fragments arranged by size:
1925 1162 209 185 181 90
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstXI CCAn_nnnn'nTGG
Cuts at: 249 615 249
Size: 366 3386
BstYI r'GATC_y
Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958
Size: 776 467 23 335 246 1012 86 12
Cuts at: 2958 3304 1
Size: 346 449
Fragments arranged by size:
1012 776 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 593 593
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 423 751 423
Size: 328 3424
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 179 251 590 596 1337 1511 1902 1929 3274
Size: 72 339 6 741 174 391 27 1345
Cuts at: 3274 179
Size: 657
Fragments arranged by size:
1345 741 657 391 339 174 72 27
6
EagI C'GGCC_G
Cuts at: 590 1337 590
Size: 747 3005
EarI CTCTTCn'nnn_
Cuts at: 547 1775 1985 547
Size: 1228 210 2314
Fragments arranged by size:
2314 1228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 494 1960 2270 2416 3562 494
Size: 1466 310 146 1146 684
Fragments arranged by size:
1466 1146 684 310 146
EcoICRI GAG'CTC
Cuts at: 234 764 234
Size: 530 3222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2009 2756 3024 1577
Size: 432 747 268 2305
Fragments arranged by size:
2305 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 159 891 159
Size: 732 3020
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 560 736 1186 1434 2456 3435 560
Size: 176 450 248 1022 979 877
Fragments arranged by size:
1022 979 877 450 248 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1624 1626 1656 1594
Size: 30 2 30 3690
Fragments arranged by size:
3690 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 732 1182 1430 732
Size: 450 248 3054
Fragments arranged by size:
3054 450 248
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 385 1741 1868 385
Size: 1356 127 2269
Fragments arranged by size:
2269 1356 127
MspA1I CmG'CkG
Cuts at: 136 595 863 1177 1537 2550 2795 136
Size: 459 268 314 360 1013 245 1093
Fragments arranged by size:
1093 1013 459 360 314 268 245
NaeI GCC'GGC
Cuts at: 315 590 1933 315
Size: 275 1343 2134
Fragments arranged by size:
2134 1343 275
NarI GG'CG_CC
Cuts at: 733 1183 1431 733
Size: 450 248 3054
Fragments arranged by size:
3054 450 248
NgoMIV G'CCGG_C
Cuts at: 313 588 1931 313
Size: 275 1343 2134
Fragments arranged by size:
2134 1343 275
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 236 766 236
Size: 530 3222
SacII CC_GC'GG
Cuts at: 596 596
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 547 1775 1985 547
Size: 1228 210 2314
Fragments arranged by size:
2314 1228 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 734 1184 1432 734
Size: 450 248 3054
Fragments arranged by size:
3054 450 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SgrAI Cr'CCGG_yG
Cuts at: 403 403
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 358 907 1822 2314 2576 358
Size: 549 915 492 262 1534
Fragments arranged by size:
1534 915 549 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 242 461 638 692 707 886 1240 242
Size: 219 177 54 15 179 354 2754
Fragments arranged by size:
2754 354 219 179 177 54 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI