Restriction map for pF1KE1218
 (Linear) (Six-base) MAPSORT of: KIEE1218.seq  Check: 2247  from: 1  to: 639



KIEE1218.orf

 With 182 enzymes: SgfI * 

                        November 11, 2016 16:52  ..

AfeI AGC'GCT

Cuts at:      0     450     639 
   Size:        450     189
 
AgeI A'CCGG_T

Cuts at:      0     295     639 
   Size:        295     344
 
AleI CACnn'nnGTG

Cuts at:      0     277     639 
   Size:        277     362
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     295     639 
   Size:        295     344
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     219     252     639 
   Size:        219      33     387

  Fragments arranged by size: 

                 387     219      33
 
BanI G'GyrC_C

Cuts at:      0     292     624     639 
   Size:        292     332      15

  Fragments arranged by size: 

                 332     292      15
 
BanII G_rGCy'C

Cuts at:      0     128     520     639 
   Size:        128     392     119

  Fragments arranged by size: 

                 392     128     119
 
BbeI G_GCGC'C

Cuts at:      0     628     639 
   Size:        628      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79     639 
   Size:         79     560
 
BlpI GC'TnA_GC

Cuts at:      0     593     639 
   Size:        593      46
 
Bme1580I G_kGCm'C

Cuts at:      0     274     639 
   Size:        274     365
 
Bpu10I CC'TnA_GC

Cuts at:      0     569     639 
   Size:        569      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     639 
   Size:        235     404
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     639 
   Size:        253     386
 
BsaHI Gr'CG_yC

Cuts at:      0     103     625     639 
   Size:        103     522      14

  Fragments arranged by size: 

                 522     103      14
 
BsaWI w'CCGG_w

Cuts at:      0     106     295     639 
   Size:        106     189     344

  Fragments arranged by size: 

                 344     189     106
 
BseYI C'CCAG_C

Cuts at:      0      82     639 
   Size:         82     557
 
BsiEI CG_ry'CG

Cuts at:      0     485     639 
   Size:        485     154
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     639 
   Size:        128     511
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     274     520     639 
   Size:        128     146     246     119

  Fragments arranged by size: 

                 246     146     128     119
 
BsrBI CCG'CTC

Cuts at:      0      22     639 
   Size:         22     617
 
BsrFI r'CCGG_y

Cuts at:      0     205     295     480     639 
   Size:        205      90     185     159

  Fragments arranged by size: 

                 205     185     159      90
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141     507     639 
   Size:        141     366     132

  Fragments arranged by size: 

                 366     141     132
 
BtgI C'CryG_G

Cuts at:      0     485     639 
   Size:        485     154
 
DraI TTT'AAA

Cuts at:      0     315     639 
   Size:        315     324
 
EaeI y'GGCC_r

Cuts at:      0      71     143     482     488     639 
   Size:         71      72     339       6     151

  Fragments arranged by size: 

                 339     151      72      71       6
 
EagI C'GGCC_G

Cuts at:      0     482     639 
   Size:        482     157
 
EarI CTCTTCn'nnn_

Cuts at:      0     439     639 
   Size:        439     200
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     386     639 
   Size:        386     253
 
EcoICRI GAG'CTC

Cuts at:      0     126     639 
   Size:        126     513
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     639 
   Size:         51     588
 
HaeII r_GCGC'y

Cuts at:      0     452     628     639 
   Size:        452     176      11

  Fragments arranged by size: 

                 452     176      11
 
KasI G'GCGC_C

Cuts at:      0     624     639 
   Size:        624      15
 
MslI CAynn'nnrTG

Cuts at:      0     277     639 
   Size:        277     362
 
MspA1I CmG'CkG

Cuts at:      0      28     487     639 
   Size:         28     459     152

  Fragments arranged by size: 

                 459     152      28
 
NaeI GCC'GGC

Cuts at:      0     207     482     639 
   Size:        207     275     157

  Fragments arranged by size: 

                 275     207     157
 
NarI GG'CG_CC

Cuts at:      0     625     639 
   Size:        625      14
 
NgoMIV G'CCGG_C

Cuts at:      0     205     480     639 
   Size:        205     275     159

  Fragments arranged by size: 

                 275     205     159
 
SacI G_AGCT'C

Cuts at:      0     128     639 
   Size:        128     511
 
SacII CC_GC'GG

Cuts at:      0     488     639 
   Size:        488     151
 
SapI GCTCTTCn'nnn_

Cuts at:      0     439     639 
   Size:        439     200
 
SfoI GGC'GCC

Cuts at:      0     626     639 
   Size:        626      13
 
SgrAI Cr'CCGG_yG

Cuts at:      0     295     639 
   Size:        295     344
 
SmlI C'TyrA_G

Cuts at:      0     250     639 
   Size:        250     389
 
StyI C'CwwG_G

Cuts at:      0     134     353     530     584     599     639 
   Size:        134     219     177      54      15      40

  Fragments arranged by size: 

                 219     177     134      54      40      15
 

 Enzymes that do cut:

     AfeI     AgeI     AleI     AlfI     BaeI     BanI    BanII     BbeI
     BglI     BlpI Bme1580I   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrFI    BstXI     BtgI
     DraI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    HaeII
     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     SacI    SacII
     SapI     SfoI    SgrAI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI     BpmI
    BsaAI    BsaBI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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