Restriction map for pF1KE1617
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRII      EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeIII      HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HphI        ItaI        KasI        
KpnI        KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MscI        
MslI        MspA1I      MunI        MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TseI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        153  TCCGGA               T^CCGGA
AcsI          192  GAATTC               [GA]^AATT[CT]
AfaI          366  GTAC                 GT^AC
Aor51HI       260  AGCGCT               AGC^GCT
ApoI          192  GAATTC               [GA]^AATT[CT]
Asp700I       192  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          346  GGCACC               G^G[CT][GA]CC
BcnI          313  CCGGG                CC^[GC]GG
BmyI          345  GGGCAC               G[AGT]GC[ACT]^C
BsaJI         350  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         153  TCCGGA               [AT]^CCGG[AT]
BseAI         153  TCCGGA               T^CCGGA
Bsp1286I      345  GGGCAC               G[AGT]GC[ACT]^C
BspEI         153  TCCGGA               T^CCGGA
BspMI         144  GCAGGT               ACCTGC(4/8)
BsrI          160  ACTGG                ACTGG(1/-1)
BssKI         313  CCGGG                ^CC[ACGT]GG
BstBI         171  TTCGAA               TT^CGAA
BstF5I        151  CATCC                GGATG(2/0)
DraII          97  AGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          168  CTCTTC               CTCTTC(1/4)
Eco47III      260  AGCGCT               AGC^GCT
EcoO109I       97  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         192  GAATTC               G^AATTC
EcoRV         208  GATATC               GAT^ATC
EcoT14I       350  CCAAGG               C^C[AT][AT]GG
FokI          151  CATCC                GGATG(9/13)
HaeII         260  AGCGCT               [GA]GCGC^[CT]
HinfI         285  GATTC                G^A[ACGT]TC
Ksp632I       168  CTCTTC               CTCTTC(1/4)
MaeII         240  ACGT                 A^CGT
MroI          153  TCCGGA               T^CCGGA
NciI          313  CCGGG                CC^[GC]GG
NlaIII        331  CATG                 CATG^
NspI          330  ACATGC               [GA]CATG^[CT]
NspV          171  TTCGAA               TT^CGAA
PpuMI          97  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI          366  GTAC                 GT^AC
ScrFI         313  CCGGG                CC^[ACGT]GG
SfcI          252  CTACAG               C^T[GA][CT]AG
SfuI          171  TTCGAA               TT^CGAA
StyI          350  CCAAGG               C^C[AT][AT]GG
TaqI          172  TCGA                 T^CGA
TfiI          285  GATTC                G^A[AT]TC
Tsp509I       193  AATT                 ^AATT
TthHB8I       172  TCGA                 T^CGA
XcmI          150  CCATCCGGACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          192  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII          98  GGACC                G^G[AT]CC
              147  GGTCC               
BsiYI          69  CCAGAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              154  CCGGACACTGG         
BslI           69  CCAGAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              154  CCGGACACTGG         
Cfr13I         98  GGACC                G^G[ACGT]CC
              147  GGTCC               
DdeI          341  CTGAG                C^T[ACGT]AG
              362  CTAAG               
MaeIII        202  GTCAC                ^GT[ACGT]AC
              358  GTCAC               
MboII         169  TCTTC                GAAGA(8/7)
              274  TCTTC               
MseI           47  TTAA                 T^TAA
               62  TTAA                
NlaIV          98  GGACCC               GG[ACGT]^[ACGT]CC
              346  GGCACC              
Sau96I         98  GGACC                G^G[ACGT]CC
              147  GGTCC               
SfaNI         164  GCATC                GCATC(5/9)
              235  GCATC               
Tru9I          47  TTAA                 T^TAA
               62  TTAA                
Tsp45I        202  GTCAC                ^GT[GC]AC
              358  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        Cac8I       CfoI        HapII       HhaI        
HinP1I      HpaII       MnlI        MspI        MwoI        
TspRI       
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