Restriction map for pF1KE1617
 (Circular) (Six-base) MAPSORT of: pF1KE1617.seq  Check: 5316  from: 1  to: 3494



pF1KE1617  3494 bp

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:    532     532 
   Size:       3494
 
Acc65I G'GTAC_C

Cuts at:    510     510 
   Size:       3494
 
AclI AA'CG_TT

Cuts at:   3334    3334 
   Size:       3494
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1319    1751    2498    2766    1319 
   Size:        432     747     268    2047

  Fragments arranged by size: 

                2047     747     432     268
 
AfeI AGC'GCT

Cuts at:    370     370 
   Size:       3494
 
AflIII A'CryG_T

Cuts at:    846    1950    2927     846 
   Size:       1104     977    1413

  Fragments arranged by size: 

                1413    1104     977
 
AgeI A'CCGG_T

Cuts at:   1882    1882 
   Size:       3494
 
AlwNI CAG_nnn'CTG

Cuts at:    799    2366     799 
   Size:       1567    1927
 
ApaLI G'TGCA_C

Cuts at:   2264    2264 
   Size:       3494
 
ApoI r'AATT_y

Cuts at:    300     498    2847    3103     300 
   Size:        198    2349     256     691

  Fragments arranged by size: 

                2349     691     256     198
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3494
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3494
 
AvaI C'yCGr_G

Cuts at:    514     514 
   Size:       3494
 
BamHI G'GATC_C

Cuts at:    519     519 
   Size:       3494
 
BanI G'GyrC_C

Cuts at:    454     510     924    1172    1207     454 
   Size:         56     414     248      35    2741

  Fragments arranged by size: 

                2741     414     248      56      35
 
BanII G_rGCy'C

Cuts at:    508    1538     508 
   Size:       1030    2464
 
BbeI G_GCGC'C

Cuts at:    928    1176     928 
   Size:        248    3246
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3460
 
BciVI GTATCCnnnnn_n'

Cuts at:   1392    2153    3494    1392 
   Size:        761    1341    1392

  Fragments arranged by size: 

                1392    1341     761
 
BclI T'GATC_A

Cuts at:   1014    1014 
   Size:       3494
 
BglII A'GATC_T

Cuts at:   1009    3046    1009 
   Size:       2037    1457
 
BlpI GC'TnA_GC

Cuts at:    606     606 
   Size:       3494
 
Bme1580I G_kGCm'C

Cuts at:    457    1119    1212    2268     457 
   Size:        662      93    1056    1683

  Fragments arranged by size: 

                1683    1056     662      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    706     787    1122    3266     706 
   Size:         81     335    2144     934

  Fragments arranged by size: 

                2144     934     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1861    1861 
   Size:       3494
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    670    1549    2041    2339     670 
   Size:        879     492     298    1825

  Fragments arranged by size: 

                1825     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3198    3198 
   Size:       3494
 
BsaAI yAC'GTr

Cuts at:    847     847 
   Size:       3494
 
BsaBI GATnn'nnATC

Cuts at:      6    1008    3045       6 
   Size:       1002    2037     455

  Fragments arranged by size: 

                2037    1002     455
 
BsaHI Gr'CG_yC

Cuts at:    925    1173    1875    3371     925 
   Size:        248     702    1496    1048

  Fragments arranged by size: 

                1496    1048     702     248
 
BsaWI w'CCGG_w

Cuts at:    261     683    1204    1882    2156    2303    2714     261 
   Size:        422     521     678     274     147     411    1041

  Fragments arranged by size: 

                1041     678     521     422     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    466     496     466 
   Size:         30    3464
 
BseYI C'CCAG_C

Cuts at:    919    1677    2254    2750     919 
   Size:        758     577     496    1663

  Fragments arranged by size: 

                1663     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1036    1082    2290     104 
   Size:        932      46    1208    1308

  Fragments arranged by size: 

                1308    1208     932      46
 
BsiHKAI G_wGCw'C

Cuts at:    508    1286    1476    2268     508 
   Size:        778     190     792    1734

  Fragments arranged by size: 

                1734     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    457     508    1119    1212    1286    1476    1538    2268     457 
   Size:         51     611      93      74     190      62     730    1683

  Fragments arranged by size: 

                1683     730     611     190      93      74      62      51
            
 
BspEI T'CCGG_A

Cuts at:    261    2714     261 
   Size:       2453    1041
 
BspHI T'CATG_A

Cuts at:   2670    2670 
   Size:       3494
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    243     544     758    1060    1441     243 
   Size:        301     214     302     381    2296

  Fragments arranged by size: 

                2296     381     302     301     214
 
BsrBI CCG'CTC

Cuts at:     50    1786    1840    3324    3489      50 
   Size:       1736      54    1484     165      55

  Fragments arranged by size: 

                1736    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1406    1406 
   Size:       3494
 
BsrFI r'CCGG_y

Cuts at:   1492    1673    1882    1492 
   Size:        181     209    3104

  Fragments arranged by size: 

                3104     209     181
 
BssSI C'ACGA_G

Cuts at:   1765    2123    1765 
   Size:        358    3136
 
BstAPI GCAn_nnn'nTGC

Cuts at:    871     871 
   Size:       3494
 
BstBI TT'CG_AA

Cuts at:    280    1855     280 
   Size:       1575    1919
 
BstYI r'GATC_y

Cuts at:      1     519     986    1009    1344    1590    2602    2688    2700 
   Size:        518     467      23     335     246    1012      86      12

Cuts at:   2700    3046       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     518     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1444    1625    1788    1444 
   Size:        181     163    3150

  Fragments arranged by size: 

                3150     181     163
 
BtsI GCAGTG_nn'

Cuts at:    410     737     410 
   Size:        327    3167
 
DraI TTT'AAA

Cuts at:    493     493 
   Size:       3494
 
DrdI GACnn_nn'nnGTC

Cuts at:   1200    2058    1200 
   Size:        858    2636
 
EaeI y'GGCC_r

Cuts at:   1079    1253    1644    1671    3016    1079 
   Size:        174     391      27    1345    1557

  Fragments arranged by size: 

                1557    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1079    1079 
   Size:       3494
 
EarI CTCTTCn'nnn_

Cuts at:    282    1517    1727     282 
   Size:       1235     210    2049

  Fragments arranged by size: 

                2049    1235     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1702    2012    2158    3304    1702 
   Size:        310     146    1146    1892

  Fragments arranged by size: 

                1892    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    506     506 
   Size:       3494
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1319    1751    2498    2766    1319 
   Size:        432     747     268    2047

  Fragments arranged by size: 

                2047     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    206     633     206 
   Size:        427    3067
 
EcoRI G'AATT_C

Cuts at:    300     498     300 
   Size:        198    3296
 
EcoRV GAT'ATC

Cuts at:    318     318 
   Size:       3494
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    168    1513     168 
   Size:       1345    2149
 
FspI TGC'GCA

Cuts at:   1275    1275 
   Size:       3494
 
HaeII r_GCGC'y

Cuts at:    372     928    1176    2198    3177     372 
   Size:        556     248    1022     979     689

  Fragments arranged by size: 

                1022     979     689     556     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    106     138    1336    1366    1368    1398     106 
   Size:         32    1198      30       2      30    2202

  Fragments arranged by size: 

                2202    1198      32      30      30       2
 
HincII GTy'rAC

Cuts at:    533     533 
   Size:       3494
 
KasI G'GCGC_C

Cuts at:    924    1172     924 
   Size:        248    3246
 
KpnI G_GTAC'C

Cuts at:    514     514 
   Size:       3494
 
MluI A'CGCG_T

Cuts at:   2927    2927 
   Size:       3494
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2165    2349    2165 
   Size:        184    3310
 
MscI TGG'CCA

Cuts at:   1255    1255 
   Size:       3494
 
MslI CAynn'nnrTG

Cuts at:   1483    1610    1483 
   Size:        127    3367
 
MspA1I CmG'CkG

Cuts at:    605     919    1279    2292    2537     605 
   Size:        314     360    1013     245    1562

  Fragments arranged by size: 

                1562    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1675    1675 
   Size:       3494
 
NarI GG'CG_CC

Cuts at:    925    1173     925 
   Size:        248    3246
 
NgoMIV G'CCGG_C

Cuts at:   1673    1673 
   Size:       3494
 
NspI r_CATG'y

Cuts at:    442     547    1047    1954     442 
   Size:        105     500     907    1982

  Fragments arranged by size: 

                1982     907     500     105
 
PciI A'CATG_T

Cuts at:   1950    1950 
   Size:       3494
 
PmeI GTTT'AAAC

Cuts at:    493     493 
   Size:       3494
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2633    2665    2633 
   Size:         32    3462
 
PpuMI rG'GwC_Cy

Cuts at:    206     206 
   Size:       3494
 
PstI C_TGCA'G

Cuts at:    541    1226     541 
   Size:        685    2809
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3494
 
PvuII CAG'CTG

Cuts at:    919    1279     919 
   Size:        360    3134
 
RsrII CG'GwC_CG

Cuts at:   1689    1689 
   Size:       3494
 
SacI G_AGCT'C

Cuts at:    508     508 
   Size:       3494
 
SalI G'TCGA_C

Cuts at:    531     531 
   Size:       3494
 
SapI GCTCTTCn'nnn_

Cuts at:   1517    1727    1517 
   Size:        210    3284
 
SbfI CC_TGCA'GG

Cuts at:    541     541 
   Size:       3494
 
ScaI AGT'ACT

Cuts at:   3011    3011 
   Size:       3494
 
SfcI C'TryA_G

Cuts at:     33     360     537    1222    2215    2406      33 
   Size:        327     177     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     327     191     177
 
SfoI GGC'GCC

Cuts at:    926    1174     926 
   Size:        248    3246
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3494
 
SmaI CCC'GGG

Cuts at:    516     516 
   Size:       3494
 
SmlI C'TyrA_G

Cuts at:    649    1564    2056    2318     649 
   Size:        915     492     262    1825

  Fragments arranged by size: 

                1825     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3494
 
SphI G_CATG'C

Cuts at:    547    1047     547 
   Size:        500    2994
 
StyI C'CwwG_G

Cuts at:    458     485     628     982     458 
   Size:         27     143     354    2970

  Fragments arranged by size: 

                2970     354     143      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1077    1210    1669    1878    1077 
   Size:        133     459     209    2693

  Fragments arranged by size: 

                2693     459     209     133
 
TatI w'GTAC_w

Cuts at:    473    3009     473 
   Size:       2536     958
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3011    3043    3011 
   Size:         32    3462
 
Tth111I GACn'n_nGTC

Cuts at:   1291    1291 
   Size:       3494
 
XbaI T'CTAG_A

Cuts at:    525     525 
   Size:       3494
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    265     989     265 
   Size:        724    2770
 
XmaI C'CCGG_G

Cuts at:    514     514 
   Size:       3494
 
XmnI GAAnn'nnTTC

Cuts at:    304     304 
   Size:       3494
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI    PpuMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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