Restriction map for
pF1KE6821
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 453 GAATTT [GA]^AATT[CT]
AgeI 685 ACCGGT A^CCGGT
ApoI 453 GAATTT [GA]^AATT[CT]
BbsI 1038 GAAGAC GAAGAC(2/6)
BglII 428 AGATCT A^GATCT
BpuAI 1038 GAAGAC GAAGAC(2/6)
BsgI 575 GTGCAG GTGCAG(16/14)
BsiEI 335 CGGTCG CG[GA][CT]^CG
BsmAI 912 GTCTC GTCTC(1/5)
BsrDI 933 CATTGC GCAATG(2/0)
BsrFI 685 ACCGGT [GA]^CCGG[CT]
BstBI 536 TTCGAA TT^CGAA
BstYI 428 AGATCT [GA]^GATC[CT]
CfoI 210 GCGC GCG^C
Cfr10I 685 ACCGGT [GA]^CCGG[CT]
EcoRV 497 GATATC GAT^ATC
HhaI 210 GCGC GCG^C
HinP1I 210 GCGC G^CGC
MaeII 461 ACGT A^CGT
MflI 428 AGATCT [GA]^GATC[CT]
MseI 178 TTAA T^TAA
MslI 351 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 468 CCATGG C^CATGG
NspV 536 TTCGAA TT^CGAA
PflMI 468 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 685 ACCGGT A^CCGGT
PleI 910 GAGTC GAGTC(4/5)
SexAI 137 ACCTGGT A^CC[AT]GGT
SfiI 403 GGCCTCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 536 TTCGAA TT^CGAA
TaqI 537 TCGA T^CGA
TfiI 534 GATTC G^A[AT]TC
Tru9I 178 TTAA T^TAA
Tsp509I 454 AATT ^AATT
TthHB8I 537 TCGA T^CGA
Van91I 468 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 428 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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Acc65I 134 GGTACC G^GTACC
227 GGTACC
AlwI 510 GATCC GGATC(4/5)
708 GGATC
ApaI 50 GGGCCC GGGCC^C
287 GGGCCC
Asp718I 134 GGTACC G^GTACC
227 GGTACC
BbvCI 1043 CCTCAGC CCTCAGC(-5/-2)
199 GCTGAGG
BfaI 106 CTAG C^TAG
151 CTAG
BglI 52 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
404 GCCTCAAGGGC
BsaWI 131 TCCGGT [AT]^CCGG[AT]
685 ACCGGT
BsrI 242 ACTGG ACTGG(1/-1)
1055 CCAGT
BstEII 90 GGTGACC G^GT[ACGT]ACC
688 GGTGACC
BstPI 90 GGTGACC G^GT[ACGT]ACC
688 GGTGACC
DsaI 282 CCGTGG C^C[GA][CT]GG
468 CCATGG
EarI 868 CTCTTC CTCTTC(1/4)
1015 GAAGAG
EcoO65I 90 GGTGACC G^GT[ACGT]ACC
688 GGTGACC
EcoT14I 65 CCTTGG C^C[AT][AT]GG
468 CCATGG
HinfI 534 GATTC G^A[ACGT]TC
910 GAGTC
HphI 90 GGTGA GGTGA(8/7)
688 GGTGA
KpnI 134 GGTACC GGTAC^C
227 GGTACC
Ksp632I 868 CTCTTC CTCTTC(1/4)
1015 GAAGAG
MaeI 106 CTAG C^TAG
151 CTAG
PvuII 163 CAGCTG CAG^CTG
422 CAGCTG
SacI 520 GAGCTC GAGCT^C
1084 GAGCTC
StyI 65 CCTTGG C^C[AT][AT]GG
468 CCATGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I TspRI