Restriction map for pF1KE9419
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsgI        BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BstBI       BstXI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MfeI        MluNI       MscI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         483  GACGTC               GACGT^C
AccIII        135  TCCGGA               T^CCGGA
AscI          175  GGCGCGCC             GG^CGCGCC
BbeI         1302  GGCGCC               GGCGC^C
BglI          673  GCCGCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         243  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         135  TCCGGA               T^CCGGA
BseRI         730  GAGGAG               GAGGAG(10/8)
BsmBI        1223  CGTCTC               CGTCTC(1/5)
BspEI         135  TCCGGA               T^CCGGA
BspLU11I      494  ACATGT               A^CATGT
BssHII        176  GCGCGC               G^CGCGC
Bst1107I     1102  GTATAC               GTA^TAC
BstAPI        807  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        969  GGTCACC              G^GT[ACGT]ACC
BstPI         969  GGTCACC              G^GT[ACGT]ACC
BstYI         659  GGATCT               [GA]^GATC[CT]
BstZ17I      1102  GTATAC               GTA^TAC
DraII        1295  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     1295  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       969  GGTCACC              G^GT[ACGT]ACC
HinfI         243  GATTC                G^A[ACGT]TC
KasI         1302  GGCGCC               G^GCGCC
MaeII         484  ACGT                 A^CGT
MamI          243  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          659  GGATCT               [GA]^GATC[CT]
MroI          135  TCCGGA               T^CCGGA
NarI         1302  GGCGCC               GG^CGCC
NcoI          476  CCATGG               C^CATGG
NheI           60  GCTAGC               G^CTAGC
NotI         1315  GCGGCCGC             GC^GGCCGC
PflMI         543  CCATTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI          672  GGCCGCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1302  GGCGCC               GGC^GCC
SmlI         1198  CTCAAG               C^T[CT][GA]AG
TfiI          243  GATTC                G^A[AT]TC
Van91I        543  CCATTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         659  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1102  GTATAC               GT^[AC][GT]AC
             1360  GTCTAC              
AfaI          261  GTAC                 GT^AC
              498  GTAC                
AlwI          659  GGATC                GGATC(4/5)
             1281  GGATC               
AlwNI         624  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1285  CAGGGGCTG           
AspHI         394  GTGCTC               G[AT]GC[AT]^C
             1273  GAGCAC              
BanI         1068  GGTGCC               G^G[CT][GA]CC
             1302  GGCGCC              
BfaI           61  CTAG                 C^TAG
             1129  CTAG                
BsiHKAI       394  GTGCTC               G[AT]GC[AT]^C
             1273  GAGCAC              
BsmAI         957  GAGAC                GTCTC(1/5)
             1224  GTCTC               
BsrI          263  ACTGG                ACTGG(1/-1)
              692  CCAGT               
BstF5I        190  CATCC                GGATG(2/0)
             1047  CATCC               
EcoNI          90  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1092  CCTGTGCCAGG         
EcoT14I       476  CCATGG               C^C[AT][AT]GG
              641  CCAAGG              
FokI          190  CATCC                GGATG(9/13)
             1047  CATCC               
KspI          917  CCGCGG               CCGC^GG
             1319  CCGCGG              
MaeI           61  CTAG                 C^TAG
             1129  CTAG                
MluI          119  ACGCGT               A^CGCGT
             1106  ACGCGT              
PstI          127  CTGCAG               CTGCA^G
             1291  CTGCAG              
PvuII         624  CAGCTG               CAG^CTG
             1143  CAGCTG              
RsaI          261  GTAC                 GT^AC
              498  GTAC                
SacII         917  CCGCGG               CCGC^GG
             1319  CCGCGG              
SapI          318  GAAGAGC              GCTCTTC(1/4)
              564  GAAGAGC             
SmaI          195  CCCGGG               CCC^GGG
              233  CCCGGG              
SphI          323  GCATGC               GCATG^C
             1241  GCATGC              
StyI          476  CCATGG               C^C[AT][AT]GG
              641  CCAAGG              
TaqI          964  TCGA                 T^CGA
             1231  TCGA                
TthHB8I       964  TCGA                 T^CGA
             1231  TCGA                
XmaCI         195  CCCGGG               C^CCGGG
              233  CCCGGG              
XmaI          195  CCCGGG               C^CCGGG
              233  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AcyI        AflIII      AluI        
AvaI        AvaII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsaWI       BsiEI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BssKI       BssSI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      TspRI       
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