Restriction map for pF1KE2419
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KspI        MaeI        MfeI        
MroI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       1505  GGTACC               G^GTACC
AcsI         1065  GAATTT               [GA]^AATT[CT]
AflIII       1740  ACGCGT               A^C[GA][CT]GT
ApoI         1065  GAATTT               [GA]^AATT[CT]
Asp718I      1505  GGTACC               G^GTACC
BcnI         1077  CCCGG                CC^[GC]GG
BglI         1405  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         783  AGATCT               A^GATCT
BsaBI        1329  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1818  CGTCTC               CGTCTC(1/5)
BsmI         2056  GAATGC               GAATGC(1/-1)
BspHI        1142  TCATGA               T^CATGA
BstEII        456  GGTGACC              G^GT[ACGT]ACC
BstPI         456  GGTGACC              G^GT[ACGT]ACC
DraII        2030  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     2030  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       456  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1436  ATGCAT               ATGCA^T
HaeII         358  AGCGCC               [GA]GCGC^[CT]
HgaI         1742  GCGTC                GACGC(5/10)
HincII       1239  GTTGAC               GT[CT]^[GA]AC
HindII       1239  GTTGAC               GT[CT]^[GA]AC
KpnI         1505  GGTACC               GGTAC^C
MamI         1329  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1740  ACGCGT               A^CGCGT
MseI          935  TTAA                 T^TAA
NaeI         1648  GCCGGC               GCC^GGC
NciI         1077  CCCGG                CC^[GC]GG
NgoMIV       1648  GCCGGC               G^CCGGC
NsiI         1436  ATGCAT               ATGCA^T
PaeR7I        721  CTCGAG               C^TCGAG
PpuMI        2030  AGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         1142  TCATGA               T^CATGA
SacI          474  GAGCTC               GAGCT^C
SapI          632  GAAGAGC              GCTCTTC(1/4)
Tru9I         935  TTAA                 T^TAA
XhoI          721  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaI          231  CTCGGG               C^[CT]CG[GA]G
              721  CTCGAG              
BclI         1139  TGATCA               T^GATCA
             1598  TGATCA              
BsaI          100  GAGACC               GGTCTC(1/5)
             1987  GAGACC              
BseRI        1443  GAGGAG               GAGGAG(10/8)
             1945  GAGGAG              
BsgI          963  CTGCAC               GTGCAG(16/14)
              469  GTGCAG              
BsmFI        1969  GTCCC                GGGAC(10/14)
             2032  GTCCC               
BsoBI         231  CTCGGG               C^[CT]CG[GA]G
              721  CTCGAG              
BsrFI        1648  GCCGGC               [GA]^CCGG[CT]
             1831  ACCGGC              
BstAPI       1922  GCAGCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1925  GCAAGTGCTGC         
Cfr10I       1648  GCCGGC               [GA]^CCGG[CT]
             1831  ACCGGC              
DsaI          164  CCACGG               C^C[GA][CT]GG
              698  CCGTGG              
EcoT14I       285  CCAAGG               C^C[AT][AT]GG
             1544  CCAAGG              
FauI         1620  CCCGC                CCCGC(4/6)
             1656  GCGGG               
FbaI         1139  TGATCA               T^GATCA
             1598  TGATCA              
PstI         1723  CTGCAG               CTGCA^G
             1920  CTGCAG              
PvuII         367  CAGCTG               CAG^CTG
              829  CAGCTG              
SfcI         1723  CTGCAG               C^T[GA][CT]AG
             1920  CTGCAG              
SmlI          721  CTCGAG               C^T[CT][GA]AG
             1171  CTCAAG              
StyI          285  CCAAGG               C^C[AT][AT]GG
             1544  CCAAGG              
TfiI          270  GATTC                G^A[AT]TC
             1334  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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