Restriction map for pF1KE2419
 (Linear) (Six-base) MAPSORT of: KIEE2419.seq  Check: 5137  from: 1  to: 2070



KIEE2419.orf

 With 182 enzymes: SgfI * 

                        June 11, 2014 14:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     597    2070 
   Size:        597    1473
 
Acc65I G'GTAC_C

Cuts at:      0    1505    2070 
   Size:       1505     565
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     384    1819    2070 
   Size:        208     176    1435     251

  Fragments arranged by size: 

                1435     251     208     176
 
AflIII A'CryG_T

Cuts at:      0    1740    2070 
   Size:       1740     330
 
AleI CACnn'nnGTG

Cuts at:      0     193    2070 
   Size:        193    1877
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      95    1117    2070 
   Size:         95    1022     953

  Fragments arranged by size: 

                1022     953      95
 
ApoI r'AATT_y

Cuts at:      0    1065    2070 
   Size:       1065    1005
 
AvaI C'yCGr_G

Cuts at:      0     231     721    2070 
   Size:        231     490    1349

  Fragments arranged by size: 

                1349     490     231
 
BanI G'GyrC_C

Cuts at:      0     126    1093    1390    1505    2070 
   Size:        126     967     297     115     565

  Fragments arranged by size: 

                 967     565     297     126     115
 
BanII G_rGCy'C

Cuts at:      0     188     478    1165    1381    2070 
   Size:        188     290     687     216     689

  Fragments arranged by size: 

                 689     687     290     216     188
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1207    1581    1680    2070 
   Size:       1207     374      99     390

  Fragments arranged by size: 

                1207     390     374      99
 
BclI T'GATC_A

Cuts at:      0    1139    1598    2070 
   Size:       1139     459     472

  Fragments arranged by size: 

                1139     472     459
 
BfrBI ATG'CAT

Cuts at:      0    1438    2070 
   Size:       1438     632
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1411    2070 
   Size:       1411     659
 
BglII A'GATC_T

Cuts at:      0     783    2070 
   Size:        783    1287
 
Bme1580I G_kGCm'C

Cuts at:      0    1096    2070 
   Size:       1096     974
 
BmgBI CAC'GTC

Cuts at:      0    1310    2070 
   Size:       1310     760
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462    1438    1744    2070 
   Size:        462     976     306     326

  Fragments arranged by size: 

                 976     462     326     306
 
Bpu10I CC'TnA_GC

Cuts at:      0    1419    2021    2070 
   Size:       1419     602      49

  Fragments arranged by size: 

                1419     602      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1156    2070 
   Size:       1156     914
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      94    1981    2070 
   Size:         94    1887      89

  Fragments arranged by size: 

                1887      94      89
 
BsaBI GATnn'nnATC

Cuts at:      0    1333    2070 
   Size:       1333     737
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     638     668     952     982    1697    1727    2070 
   Size:        638      30     284      30     715      30     343

  Fragments arranged by size: 

                 715     638     343     284      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1458    1960    2070 
   Size:       1458     502     110

  Fragments arranged by size: 

                1458     502     110
 
BseYI C'CCAG_C

Cuts at:      0      29     734    1374    1551    2070 
   Size:         29     705     640     177     519

  Fragments arranged by size: 

                 705     640     519     177      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     948    2070 
   Size:        490     458    1122

  Fragments arranged by size: 

                1122     490     458
 
BsiHKAI G_wGCw'C

Cuts at:      0     340     478     692    1172    2070 
   Size:        340     138     214     480     898

  Fragments arranged by size: 

                 898     480     340     214     138
 
BsmI GAATG_Cn'

Cuts at:      0    2062    2070 
   Size:       2062       8
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1824    2070 
   Size:       1824     246
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     340     478     692    1096    1165    1172    1381 
   Size:        188     152     138     214     404      69       7     209

Cuts at:   1381    2070 
   Size:        689

  Fragments arranged by size: 

                 689     404     214     209     188     152     138      69
                   7
 
BspHI T'CATG_A

Cuts at:      0    1142    2070 
   Size:       1142     928
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      55     597    1170    2070 
   Size:         55     542     573     900

  Fragments arranged by size: 

                 900     573     542      55
 
BsrDI GCAATG_nn'

Cuts at:      0     520     866    1440    2070 
   Size:        520     346     574     630

  Fragments arranged by size: 

                 630     574     520     346
 
BsrFI r'CCGG_y

Cuts at:      0    1648    1831    2070 
   Size:       1648     183     239

  Fragments arranged by size: 

                1648     239     183
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1928    1931    2070 
   Size:       1928       3     139

  Fragments arranged by size: 

                1928     139       3
 
BstEII G'GTnAC_C

Cuts at:      0     456    2070 
   Size:        456    1614
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195     292     373     555    1539    1551    2070 
   Size:        195      97      81     182     984      12     519

  Fragments arranged by size: 

                 984     519     195     182      97      81      12
 
BstYI r'GATC_y

Cuts at:      0     783    1080    1154    2070 
   Size:        783     297      74     916

  Fragments arranged by size: 

                 916     783     297      74
 
BtgI C'CryG_G

Cuts at:      0     164     698    2070 
   Size:        164     534    1372

  Fragments arranged by size: 

                1372     534     164
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1023    2070 
   Size:       1023    1047
 
BtsI GCAGTG_nn'

Cuts at:      0      77    2070 
   Size:         77    1993
 
EaeI y'GGCC_r

Cuts at:      0     707    1253    1412    1541    1646    2070 
   Size:        707     546     159     129     105     424

  Fragments arranged by size: 

                 707     546     424     159     129     105
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     522     627    2070 
   Size:        227     295     105    1443

  Fragments arranged by size: 

                1443     295     227     105
 
EcoICRI GAG'CTC

Cuts at:      0     476    2070 
   Size:        476    1594
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     384     462    1438    1744    1819    2070 
   Size:        208     176      78     976     306      75     251

  Fragments arranged by size: 

                 976     306     251     208     176      78      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2031    2070 
   Size:       2031      39
 
HaeII r_GCGC'y

Cuts at:      0     362    2070 
   Size:        362    1708
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     638     670    1347    1379    2070 
   Size:        638      32     677      32     691

  Fragments arranged by size: 

                 691     677     638      32      32
 
HincII GTy'rAC

Cuts at:      0    1241    2070 
   Size:       1241     829
 
KpnI G_GTAC'C

Cuts at:      0    1509    2070 
   Size:       1509     561
 
MluI A'CGCG_T

Cuts at:      0    1740    2070 
   Size:       1740     330
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     446     964    1406    1740    2070 
   Size:        354      92     518     442     334     330

  Fragments arranged by size: 

                 518     442     354     334     330      92
 
MscI TGG'CCA

Cuts at:      0     709    1255    1543    2070 
   Size:        709     546     288     527

  Fragments arranged by size: 

                 709     546     527     288
 
MslI CAynn'nnrTG

Cuts at:      0     193     553     607    1537    2070 
   Size:        193     360      54     930     533

  Fragments arranged by size: 

                 930     533     360     193      54
 
MspA1I CmG'CkG

Cuts at:      0     369     831    1623    1782    1919    2070 
   Size:        369     462     792     159     137     151

  Fragments arranged by size: 

                 792     462     369     159     151     137
 
NaeI GCC'GGC

Cuts at:      0    1650    2070 
   Size:       1650     420
 
NgoMIV G'CCGG_C

Cuts at:      0    1648    2070 
   Size:       1648     422
 
NsiI A_TGCA'T

Cuts at:      0    1440    2070 
   Size:       1440     630
 
PasI CC'CwG_GG

Cuts at:      0    1805    2070 
   Size:       1805     265
 
PfoI T'CCnGG_A

Cuts at:      0    1076    2070 
   Size:       1076     994
 
PpuMI rG'GwC_Cy

Cuts at:      0    2031    2070 
   Size:       2031      39
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1564    1596    2070 
   Size:       1564      32     474

  Fragments arranged by size: 

                1564     474      32
 
PstI C_TGCA'G

Cuts at:      0    1727    1924    2070 
   Size:       1727     197     146

  Fragments arranged by size: 

                1727     197     146
 
PvuII CAG'CTG

Cuts at:      0     369     831    2070 
   Size:        369     462    1239

  Fragments arranged by size: 

                1239     462     369
 
SacI G_AGCT'C

Cuts at:      0     478    2070 
   Size:        478    1592
 
SapI GCTCTTCn'nnn_

Cuts at:      0     627    2070 
   Size:        627    1443
 
SfcI C'TryA_G

Cuts at:      0    1723    1920    2070 
   Size:       1723     197     150

  Fragments arranged by size: 

                1723     197     150
 
SmlI C'TyrA_G

Cuts at:      0     721    1171    2070 
   Size:        721     450     899

  Fragments arranged by size: 

                 899     721     450
 
StyI C'CwwG_G

Cuts at:      0     285    1544    2070 
   Size:        285    1259     526

  Fragments arranged by size: 

                1259     526     285
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     727    2068    2070 
   Size:        727    1341       2

  Fragments arranged by size: 

                1341     727       2
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1325    1357    2070 
   Size:       1325      32     713

  Fragments arranged by size: 

                1325     713      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     429     763    1407    2070 
   Size:        429     334     644     663

  Fragments arranged by size: 

                 663     644     429     334
 
XhoI C'TCGA_G

Cuts at:      0     721    2070 
   Size:        721    1349
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI     ApoI     AvaI
     BanI    BanII     BbsI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I
   HincII     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NsiI     PasI     PfoI    PpuMI     PsrI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     StyI    TaqII     TstI     XcmI
     XhoI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BlpI     BmrI     BmtI
     BplI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BsrBI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XmaI     XmnI     ZraI
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