Restriction map for
pF1KE2457
(Linear) (Six-base) MAPSORT of: KIEE2457.seq Check: 4669 from: 1 to: 3528
KIEE2457.orf
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2737 3256 3311 3528
Size: 2737 519 55 217
Fragments arranged by size:
2737 519 217 55
AccI GT'mk_AC
Cuts at: 0 3224 3528
Size: 3224 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1006 1435 1818 2763 3174 3199 3528
Size: 121 885 429 383 945 411 25 329
Fragments arranged by size:
945 885 429 411 383 329 121 25
AfeI AGC'GCT
Cuts at: 0 787 3528
Size: 787 2741
AflII C'TTAA_G
Cuts at: 0 2808 3528
Size: 2808 720
AflIII A'CryG_T
Cuts at: 0 3392 3528
Size: 3392 136
AleI CACnn'nnGTG
Cuts at: 0 3250 3528
Size: 3250 278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 817 3528
Size: 202 615 2711
Fragments arranged by size:
2711 615 202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 1012 1044 3528
Size: 262 32 718 32 2484
Fragments arranged by size:
2484 718 262 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 984 1224 2003 2706 2756 3048 3303 3528
Size: 984 240 779 703 50 292 255 225
Fragments arranged by size:
984 779 703 292 255 240 225 50
ApaI G_GGCC'C
Cuts at: 0 636 1166 1425 1537 3019 3317 3420 3528
Size: 636 530 259 112 1482 298 103 108
Fragments arranged by size:
1482 636 530 298 259 112 108 103
ApaLI G'TGCA_C
Cuts at: 0 2724 3528
Size: 2724 804
ApoI r'AATT_y
Cuts at: 0 897 3528
Size: 897 2631
AvaI C'yCGr_G
Cuts at: 0 685 967 1771 2541 2958 3011 3528
Size: 685 282 804 770 417 53 517
Fragments arranged by size:
804 770 685 517 417 282 53
BanI G'GyrC_C
Cuts at: 0 262 2485 3528
Size: 262 2223 1043
Fragments arranged by size:
2223 1043 262
BanII G_rGCy'C
Cuts at: 0 636 1166 1425 1537 1799 1873 2273 2512
Size: 636 530 259 112 262 74 400 239
Cuts at: 2512 2632 3019 3104 3317 3420 3528
Size: 120 387 85 213 103 108
Fragments arranged by size:
636 530 400 387 262 259 239 213
120 112 108 103 85 74
BbeI G_GCGC'C
Cuts at: 0 266 3528
Size: 266 3262
BbsI GAAGACnn'nnnn_
Cuts at: 0 109 1631 3111 3146 3528
Size: 109 1522 1480 35 382
Fragments arranged by size:
1522 1480 382 109 35
BbvCI CC'TCA_GC
Cuts at: 0 2225 3515 3528
Size: 2225 1290 13
Fragments arranged by size:
2225 1290 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2939 2973 3369 3403 3528
Size: 2939 34 396 34 125
Fragments arranged by size:
2939 396 125 34 34
BfrBI ATG'CAT
Cuts at: 0 2048 3528
Size: 2048 1480
BglI GCCn_nnn'nGGC
Cuts at: 0 475 631 1002 1507 1679 1931 2854 2875
Size: 475 156 371 505 172 252 923 21
Cuts at: 2875 3014 3312 3528
Size: 139 298 216
Fragments arranged by size:
923 505 475 371 298 252 216 172
156 139 21
BlpI GC'TnA_GC
Cuts at: 0 1476 3528
Size: 1476 2052
Bme1580I G_kGCm'C
Cuts at: 0 12 636 1166 1278 1425 1537 2488 2728
Size: 12 624 530 112 147 112 951 240
Cuts at: 2728 3019 3317 3420 3528
Size: 291 298 103 108
Fragments arranged by size:
951 624 530 298 291 240 147 112
112 108 103 12
BmgBI CAC'GTC
Cuts at: 0 777 3114 3144 3528
Size: 777 2337 30 384
Fragments arranged by size:
2337 777 384 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 2606 2896 3302 3528
Size: 669 1937 290 406 226
Fragments arranged by size:
1937 669 406 290 226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 1013 3528
Size: 259 754 2515
Fragments arranged by size:
2515 754 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 295 1246 1506 2332 2347 2356 2476
Size: 36 259 951 260 826 15 9 120
Cuts at: 2476 3528
Size: 1052
Fragments arranged by size:
1052 951 826 260 259 120 36 15
9
Bpu10I CC'TnA_GC
Cuts at: 0 40 789 1669 2225 3376 3515 3528
Size: 40 749 880 556 1151 139 13
Fragments arranged by size:
1151 880 749 556 139 40 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 3528
Size: 1579 1949
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 1695 3528
Size: 1032 663 1833
Fragments arranged by size:
1833 1032 663
BsaAI yAC'GTr
Cuts at: 0 647 1194 1302 3528
Size: 647 547 108 2226
Fragments arranged by size:
2226 647 547 108
BsaBI GATnn'nnATC
Cuts at: 0 2986 3528
Size: 2986 542
BsaHI Gr'CG_yC
Cuts at: 0 263 3528
Size: 263 3265
BsaWI w'CCGG_w
Cuts at: 0 1657 3528
Size: 1657 1871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 289 319 1012 1042 1420 1450
Size: 181 30 78 30 693 30 378 30
Cuts at: 1450 2137 2167 2986 3016 3528
Size: 687 30 819 30 512
Fragments arranged by size:
819 693 687 512 378 181 78 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 248 336 1154 1428 1743 1891 2439
Size: 33 215 88 818 274 315 148 548
Cuts at: 2439 2542 3105 3112 3528
Size: 103 563 7 416
Fragments arranged by size:
818 563 548 416 315 274 215 148
103 88 33 7
BseYI C'CCAG_C
Cuts at: 0 175 782 857 1006 1010 1094 1398 1709
Size: 175 607 75 149 4 84 304 311
Cuts at: 1709 2736 2767 3438 3528
Size: 1027 31 671 90
Fragments arranged by size:
1027 671 607 311 304 175 149 90
84 75 31 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1216 2241 2351 3282 3528
Size: 1140 76 1025 110 931 246
Fragments arranged by size:
1140 1025 931 246 110 76
BsiEI CG_ry'CG
Cuts at: 0 2496 3528
Size: 2496 1032
BsiHKAI G_wGCw'C
Cuts at: 0 806 2273 2632 2728 3175 3365 3374 3528
Size: 806 1467 359 96 447 190 9 154
Fragments arranged by size:
1467 806 447 359 190 154 96 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 111 535 970 1636 1725 3528
Size: 111 424 435 666 89 1803
Fragments arranged by size:
1803 666 435 424 111 89
Bsp1286I G_dGCh'C
Cuts at: 0 12 636 806 1166 1278 1425 1537 1799
Size: 12 624 170 360 112 147 112 262
Cuts at: 1799 1873 2273 2488 2512 2632 2728 3019 3104
Size: 74 400 215 24 120 96 291 85
Cuts at: 3104 3175 3317 3365 3374 3420 3528
Size: 71 142 48 9 46 108
Fragments arranged by size:
624 400 360 291 262 215 170 147
142 120 112 112 108 96 85 74
71 48 46 24 12 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 2737 3256 3311 3528
Size: 719 2018 519 55 217
Fragments arranged by size:
2018 719 519 217 55
BsrBI CCG'CTC
Cuts at: 0 197 758 1520 3239 3528
Size: 197 561 762 1719 289
Fragments arranged by size:
1719 762 561 289 197
BsrDI GCAATG_nn'
Cuts at: 0 2226 2394 2406 3528
Size: 2226 168 12 1122
Fragments arranged by size:
2226 1122 168 12
BsrFI r'CCGG_y
Cuts at: 0 321 798 2952 3528
Size: 321 477 2154 576
Fragments arranged by size:
2154 576 477 321
BsrGI T'GTAC_A
Cuts at: 0 2420 3528
Size: 2420 1108
BssSI C'ACGA_G
Cuts at: 0 2094 3528
Size: 2094 1434
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2344 3528
Size: 2344 1184
BstBI TT'CG_AA
Cuts at: 0 836 3528
Size: 836 2692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 760 1238 1530 2310 2385 2439 3528
Size: 760 478 292 780 75 54 1089
Fragments arranged by size:
1089 780 760 478 292 75 54
Bsu36I CC'TnA_GG
Cuts at: 0 1874 3528
Size: 1874 1654
BtgI C'CryG_G
Cuts at: 0 293 716 2852 3053 3085 3282 3528
Size: 293 423 2136 201 32 197 246
Fragments arranged by size:
2136 423 293 246 201 197 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1482 3164 3212 3528
Size: 1482 1682 48 316
Fragments arranged by size:
1682 1482 316 48
BtsI GCAGTG_nn'
Cuts at: 0 179 775 985 2550 2685 3528
Size: 179 596 210 1565 135 843
Fragments arranged by size:
1565 843 596 210 179 135
ClaI AT'CG_AT
Cuts at: 0 2939 3528
Size: 2939 589
DrdI GACnn_nn'nnGTC
Cuts at: 0 93 2304 3528
Size: 93 2211 1224
Fragments arranged by size:
2211 1224 93
EaeI y'GGCC_r
Cuts at: 0 381 1694 2493 3050 3528
Size: 381 1313 799 557 478
Fragments arranged by size:
1313 799 557 478 381
EagI C'GGCC_G
Cuts at: 0 2493 3528
Size: 2493 1035
EarI CTCTTCn'nnn_
Cuts at: 0 250 319 982 2831 3528
Size: 250 69 663 1849 697
Fragments arranged by size:
1849 697 663 250 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1646 1746 3528
Size: 1646 100 1782
Fragments arranged by size:
1782 1646 100
EcoICRI GAG'CTC
Cuts at: 0 2271 2630 3528
Size: 2271 359 898
Fragments arranged by size:
2271 898 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 121 295 1006 1246 1435 1506 1818
Size: 36 85 174 711 240 189 71 312
Cuts at: 1818 2332 2347 2356 2476 2763 3174 3199 3528
Size: 514 15 9 120 287 411 25 329
Fragments arranged by size:
711 514 411 329 312 287 240 189
174 120 85 71 36 25 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 230 1450 3528
Size: 230 1220 2078
Fragments arranged by size:
2078 1220 230
EcoO109I rG'GnC_Cy
Cuts at: 0 24 270 459 633 731 1098 1163 1322
Size: 24 246 189 174 98 367 65 159
Cuts at: 1322 1350 1421 1534 2891 3015 3314 3416 3417
Size: 28 71 113 1357 124 299 102 1
Cuts at: 3417 3528
Size: 111
Fragments arranged by size:
1357 367 299 246 189 174 159 124
113 111 102 98 71 65 28 24
1
EcoRI G'AATT_C
Cuts at: 0 897 3528
Size: 897 2631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2373 3204 3528
Size: 2373 831 324
Fragments arranged by size:
2373 831 324
FspI TGC'GCA
Cuts at: 0 2071 3528
Size: 2071 1457
HaeII r_GCGC'y
Cuts at: 0 266 789 3528
Size: 266 523 2739
Fragments arranged by size:
2739 523 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 1420 1452 3528
Size: 289 32 1099 32 2076
Fragments arranged by size:
2076 1099 289 32 32
HincII GTy'rAC
Cuts at: 0 2793 2865 3528
Size: 2793 72 663
Fragments arranged by size:
2793 663 72
KasI G'GCGC_C
Cuts at: 0 262 3528
Size: 262 3266
MluI A'CGCG_T
Cuts at: 0 3392 3528
Size: 3392 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1098 1763 1982 2048 2809 2997 3528
Size: 926 172 665 219 66 761 188 531
Fragments arranged by size:
926 761 665 531 219 188 172 66
MscI TGG'CCA
Cuts at: 0 383 3528
Size: 383 3145
MslI CAynn'nnrTG
Cuts at: 0 184 2399 3250 3528
Size: 184 2215 851 278
Fragments arranged by size:
2215 851 278 184
MspA1I CmG'CkG
Cuts at: 0 205 1010 1116 1250 1950 2255 2756 3045
Size: 205 805 106 134 700 305 501 289
Cuts at: 3045 3055 3320 3326 3514 3528
Size: 10 265 6 188 14
Fragments arranged by size:
805 700 501 305 289 265 205 188
134 106 14 10 6
NaeI GCC'GGC
Cuts at: 0 323 3528
Size: 323 3205
NarI GG'CG_CC
Cuts at: 0 263 3528
Size: 263 3265
NcoI C'CATG_G
Cuts at: 0 2852 3282 3528
Size: 2852 430 246
Fragments arranged by size:
2852 430 246
NgoMIV G'CCGG_C
Cuts at: 0 321 3528
Size: 321 3207
NsiI A_TGCA'T
Cuts at: 0 2050 3528
Size: 2050 1478
NspI r_CATG'y
Cuts at: 0 73 2904 3528
Size: 73 2831 624
Fragments arranged by size:
2831 624 73
PasI CC'CwG_GG
Cuts at: 0 29 1166 1864 3420 3528
Size: 29 1137 698 1556 108
Fragments arranged by size:
1556 1137 698 108 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 1224 2706 3048 3528
Size: 1224 1482 342 480
Fragments arranged by size:
1482 1224 480 342
PmlI CAC'GTG
Cuts at: 0 647 1194 1302 3528
Size: 647 547 108 2226
Fragments arranged by size:
2226 647 547 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 3528
Size: 1012 32 2484
Fragments arranged by size:
2484 1012 32
PpuMI rG'GwC_Cy
Cuts at: 0 24 270 459 731 1350 2891 3528
Size: 24 246 189 272 619 1541 637
Fragments arranged by size:
1541 637 619 272 246 189 24
PshAI GACnn'nnGTC
Cuts at: 0 2386 3528
Size: 2386 1142
PspOMI G'GGCC_C
Cuts at: 0 632 1162 1421 1533 3015 3313 3416 3528
Size: 632 530 259 112 1482 298 103 112
Fragments arranged by size:
1482 632 530 298 259 112 112 103
PstI C_TGCA'G
Cuts at: 0 926 980 1156 2949 3325 3528
Size: 926 54 176 1793 376 203
Fragments arranged by size:
1793 926 376 203 176 54
PvuII CAG'CTG
Cuts at: 0 205 1010 1116 1950 2255 3045 3326 3514
Size: 205 805 106 834 305 790 281 188
Cuts at: 3514 3528
Size: 14
Fragments arranged by size:
834 805 790 305 281 205 188 106
14
SacI G_AGCT'C
Cuts at: 0 2273 2632 3528
Size: 2273 359 896
Fragments arranged by size:
2273 896 359
SacII CC_GC'GG
Cuts at: 0 3056 3528
Size: 3056 472
SanDI GG'GwC_CC
Cuts at: 0 24 459 1350 2891 3528
Size: 24 435 891 1541 637
Fragments arranged by size:
1541 891 637 435 24
SapI GCTCTTCn'nnn_
Cuts at: 0 982 3528
Size: 982 2546
SexAI A'CCwGG_T
Cuts at: 0 1022 3218 3528
Size: 1022 2196 310
Fragments arranged by size:
2196 1022 310
SfcI C'TryA_G
Cuts at: 0 922 930 976 1152 2945 3225 3321 3528
Size: 922 8 46 176 1793 280 96 207
Fragments arranged by size:
1793 922 280 207 176 96 46 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 631 3528
Size: 631 2897
SfoI GGC'GCC
Cuts at: 0 264 3528
Size: 264 3264
SmlI C'TyrA_G
Cuts at: 0 967 1558 2808 3528
Size: 967 591 1250 720
Fragments arranged by size:
1250 967 720 591
StuI AGG'CCT
Cuts at: 0 911 1925 2578 3520 3528
Size: 911 1014 653 942 8
Fragments arranged by size:
1014 942 911 653 8
StyI C'CwwG_G
Cuts at: 0 339 915 1204 1367 1929 2852 3282 3528
Size: 339 576 289 163 562 923 430 246
Fragments arranged by size:
923 576 562 430 339 289 246 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 2316 3528
Size: 21 2295 1212
Fragments arranged by size:
2295 1212 21
TatI w'GTAC_w
Cuts at: 0 2210 2420 3528
Size: 2210 210 1108
Fragments arranged by size:
2210 1108 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 3528
Size: 181 32 3315
Fragments arranged by size:
3315 181 32
Tth111I GACn'n_nGTC
Cuts at: 0 3115 3220 3528
Size: 3115 105 308
Fragments arranged by size:
3115 308 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1334 2355 2557 3111 3528
Size: 1334 1021 202 554 417
Fragments arranged by size:
1334 1021 554 417 202
XhoI C'TCGA_G
Cuts at: 0 967 3528
Size: 967 2561
XmnI GAAnn'nnTTC
Cuts at: 0 2776 2940 3528
Size: 2776 164 588
Fragments arranged by size:
2776 588 164
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI BtsI
ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PflMI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI SexAI SfcI SfiI SfoI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BglII BmtI BsiWI
BsmI BspEI BspHI BssHII BstEII BstYI BstZ17I CspCI
DraI DraIII EcoRV FseI FspAI HindIII HpaI KpnI
MfeI NdeI NheI NotI NruI PacI PciI PfoI
PmeI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XmaI ZraI