Restriction map for pF1KE2320
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BcgI        BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MluI        
MluNI       MroI        MscI        MspA1I      NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        579  GGTACC               G^GTACC
AccI          406  GTCTAC               GT^[AC][GT]AC
AcyI          150  GGCGCC               G[GA]^CG[CT]C
AflIII        251  ACGTGT               A^C[GA][CT]GT
AlwNI        1420  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          376  CCTCAGG              CC^T[ACGT]AGG
Asp700I      1692  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       579  GGTACC               G^GTACC
BbeI          150  GGCGCC               GGCGC^C
BbsI          563  GAAGAC               GAAGAC(2/6)
BfaI          500  CTAG                 C^TAG
BglI          277  GCCAAGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         563  GAAGAC               GAAGAC(2/6)
BsaHI         150  GGCGCC               G[GA]^CG[CT]C
BsaI         1612  GAGACC               GGTCTC(1/5)
BsgI         1326  CTGCAC               GTGCAG(16/14)
Bsp1407I      626  TGTACA               T^GTACA
BsrFI        1529  ACCGGC               [GA]^CCGG[CT]
BsrGI         626  TGTACA               T^GTACA
Bsu36I        376  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1529  ACCGGC               [GA]^CCGG[CT]
DsaI         1457  CCATGG               C^C[GA][CT]GG
EaeI          284  CGGCCA               [CT]^GGCC[GA]
EarI          764  GAAGAG               CTCTTC(1/4)
Eco81I        376  CCTCAGG              CC^T[ACGT]AGG
EcoT14I      1457  CCATGG               C^C[AT][AT]GG
Hin1I         150  GGCGCC               G[GA]^CG[CT]C
KasI          150  GGCGCC               G^GCGCC
KpnI          579  GGTACC               GGTAC^C
Ksp632I       764  GAAGAG               CTCTTC(1/4)
MaeI          500  CTAG                 C^TAG
MfeI         1010  CAATTG               C^AATTG
MseI          682  TTAA                 T^TAA
MunI         1010  CAATTG               C^AATTG
NarI          150  GGCGCC               GG^CGCC
NcoI         1457  CCATGG               C^CATGG
NdeI         1091  CATATG               CA^TATG
PflMI        1249  CCAATGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI         1589  CTACAG               C^T[GA][CT]AG
SfoI          150  GGCGCC               GGC^GCC
SmaI         1616  CCCGGG               CCC^GGG
SspBI         626  TGTACA               T^GTACA
StyI         1457  CCATGG               C^C[AT][AT]GG
Tru9I         682  TTAA                 T^TAA
Van91I       1249  CCAATGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1457  CCATGGATGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1616  CCCGGG               C^CCGGG
XmaI         1616  CCCGGG               C^CCGGG
XmnI         1692  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII         132  CGCG                 CG^CG
              394  CGCG                
AspHI         270  GAGCAC               G[AT]GC[AT]^C
              421  GAGCAC              
AvaI         1616  CCCGGG               C^[CT]CG[GA]G
             1679  CCCGAG              
BanII          61  GGGCTC               G[GA]GC[CT]^C
             1602  GGGCTC              
BbvCI        1000  CCTCAGC              CCTCAGC(-5/-2)
             1168  CCTCAGC             
BciVI         869  GTATCC               GTATCC(6/5)
             1344  GTATCC              
BlpI          418  GCTGAGC              GC^T[ACGT]AGC
              462  GCTGAGC             
BpmI         1605  CTCCAG               CTGGAG(16/14)
             1256  CTGGAG              
Bpu1102I      418  GCTGAGC              GC^T[ACGT]AGC
              462  GCTGAGC             
BsiHKAI       270  GAGCAC               G[AT]GC[AT]^C
              421  GAGCAC              
BsoBI        1616  CCCGGG               C^[CT]CG[GA]G
             1679  CCCGAG              
BstUI         132  CGCG                 CG^CG
              394  CGCG                
CelII         418  GCTGAGC              GC^T[ACGT]AGC
              462  GCTGAGC             
FauI          205  CCCGC                CCCGC(4/6)
              237  CCCGC               
HaeII         150  GGCGCC               [GA]GCGC^[CT]
              842  AGCGCC              
HgaI          728  GACGC                GACGC(5/10)
              143  GCGTC               
MvnI          132  CGCG                 CG^CG
              394  CGCG                
Tsp45I        145  GTCAC                ^GT[GC]AC
              427  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRI       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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