Restriction map for pF1KE9545
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BbeI        
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaWI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eco47III    Eco81I      FbaI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MluI        MroI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         1270  GTCTAC               GT^[AC][GT]AC
AccII        2235  CGCG                 CG^CG
AcyI          442  GGCGTC               G[GA]^CG[CT]C
AhdI         1762  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       531  GAATCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1762  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          881  TGGCCA               TGG^CCA
BamHI        2719  GGATCC               G^GATCC
BbsI         2761  GAAGAC               GAAGAC(2/6)
BciVI         280  GGATAC               GTATCC(6/5)
BpuAI        2761  GAAGAC               GAAGAC(2/6)
BsaHI         442  GGCGTC               G[GA]^CG[CT]C
BsiEI         658  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1183  TGTACA               T^GTACA
BsrFI        2043  GCCGGC               [GA]^CCGG[CT]
BsrGI        1183  TGTACA               T^GTACA
BssSI        2296  CACGAG               CACGAG(-5/-1)
BstUI        2235  CGCG                 CG^CG
Cfr10I       2043  GCCGGC               [GA]^CCGG[CT]
EagI          658  CGGCCG               C^GGCCG
Eam1105I     1762  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         658  CGGCCG               C^GGCCG
Eco52I        658  CGGCCG               C^GGCCG
EcoT22I      2079  ATGCAT               ATGCA^T
Hin1I         442  GGCGTC               G[GA]^CG[CT]C
MfeI          632  CAATTG               C^AATTG
MluNI         881  TGGCCA               TGG^CCA
MscI          881  TGGCCA               TGG^CCA
MunI          632  CAATTG               C^AATTG
MvnI         2235  CGCG                 CG^CG
NaeI         2043  GCCGGC               GCC^GGC
NgoMIV       2043  GCCGGC               G^CCGGC
NsiI         2079  ATGCAT               ATGCA^T
PvuII         638  CAGCTG               CAG^CTG
SacI         1945  GAGCTC               GAGCT^C
SexAI        1451  ACCTGGT              A^CC[AT]GGT
SfiI          882  GGCCAGCGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2654  CCCGGG               CCC^GGG
SphI         2429  GCATGC               GCATG^C
SspBI        1183  TGTACA               T^GTACA
XmaCI        2654  CCCGGG               C^CCGGG
XmaI         2654  CCCGGG               C^CCGGG
XmnI          531  GAATCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI         2654  CCCGGG               C^[CT]CG[GA]G
             2779  CCCGAG              
BbrPI         934  CACGTG               CAC^GTG
             2289  CACGTG              
BbvCI         519  CCTCAGC              CCTCAGC(-5/-2)
              688  GCTGAGG             
BcgI         2054  GCATCAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2597  GCAACACCATCG        
BsaAI         934  CACGTG               [CT]AC^GT[GA]
             2289  CACGTG              
BsaI         1213  GAGACC               GGTCTC(1/5)
             3113  GAGACC              
BsgI         2190  CTGCAC               GTGCAG(16/14)
             1346  GTGCAG              
BsmBI         444  CGTCTC               CGTCTC(1/5)
             2679  CGTCTC              
BsoBI        2654  CCCGGG               C^[CT]CG[GA]G
             2779  CCCGAG              
BsrDI        1278  CATTGC               GCAATG(2/0)
             1794  CATTGC              
BstAPI       2072  GCATCTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2169  GCAGTTCCTGC         
EcoRV         700  GATATC               GAT^ATC
             1245  GATATC              
HgaI          273  GACGC                GACGC(5/10)
              443  GCGTC               
MseI         1067  TTAA                 T^TAA
             1514  TTAA                
NcoI          587  CCATGG               C^CATGG
             2957  CCATGG              
NspI          759  GCATGT               [GA]CATG^[CT]
             2429  GCATGC              
PmaCI         934  CACGTG               CAC^GTG
             2289  CACGTG              
PmlI          934  CACGTG               CAC^GTG
             2289  CACGTG              
SapI          474  GCTCTTC              GCTCTTC(1/4)
             1326  GCTCTTC             
ScaI          734  AGTACT               AGT^ACT
              926  AGTACT              
SmlI          826  CTTGAG               C^T[CT][GA]AG
             2244  CTCAAG              
StuI          317  AGGCCT               AGG^CCT
              890  AGGCCT              
Tru9I        1067  TTAA                 T^TAA
             1514  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BglI        BglII       
BmyI        BpmI        BsaBI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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