Restriction map for pF1KE9545
 (Linear) (Six-base) MAPSORT of: KIEE9545.seq  Check: 2818  from: 1  to: 3234



KIEE9545.orf

 With 182 enzymes: SgfI * 

                        March 10, 2017 10:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1416    1687    2347    3234 
   Size:       1416     271     660     887

  Fragments arranged by size: 

                1416     887     660     271
 
AccI GT'mk_AC

Cuts at:      0    1271    3234 
   Size:       1271    1963
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     699     985    1021    1402    2409    3161    3234 
   Size:        699     286      36     381    1007     752      73

  Fragments arranged by size: 

                1007     752     699     381     286      73      36
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1767    3234 
   Size:       1767    1467
 
AleI CACnn'nnGTG

Cuts at:      0     454    3234 
   Size:        454    2780
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      68    2215    3234 
   Size:         68    2147    1019

  Fragments arranged by size: 

                2147    1019      68
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1771    1803    3234 
   Size:       1771      32    1431

  Fragments arranged by size: 

                1771    1431      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1380    1742    2175    2265    2805    2841    3234 
   Size:       1380     362     433      90     540      36     393

  Fragments arranged by size: 

                1380     540     433     393     362      90      36
 
ApoI r'AATT_y

Cuts at:      0     673     780     994    2970    3228    3234 
   Size:        673     107     214    1976     258       6

  Fragments arranged by size: 

                1976     673     258     214     107       6
 
AvaI C'yCGr_G

Cuts at:      0    2654    2779    3234 
   Size:       2654     125     455

  Fragments arranged by size: 

                2654     455     125
 
BamHI G'GATC_C

Cuts at:      0    2719    3234 
   Size:       2719     515
 
BanI G'GyrC_C

Cuts at:      0     946    1481    1608    3234 
   Size:        946     535     127    1626

  Fragments arranged by size: 

                1626     946     535     127
 
BanII G_rGCy'C

Cuts at:      0      78     110     476     833    1328    1676    1832    1949 
   Size:         78      32     366     357     495     348     156     117

Cuts at:   1949    2015    2162    2305    3234 
   Size:         66     147     143     929

  Fragments arranged by size: 

                 929     495     366     357     348     156     147     143
                 117      78      66      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2768    3234 
   Size:       2768     466
 
BbvCI CC'TCA_GC

Cuts at:      0     520     689    3234 
   Size:        520     169    2545

  Fragments arranged by size: 

                2545     520     169
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2043    2077    2586    2620    3234 
   Size:       2043      34     509      34     614

  Fragments arranged by size: 

                2043     614     509      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     274    3234 
   Size:        274    2960
 
BfrBI ATG'CAT

Cuts at:      0    2081    3234 
   Size:       2081    1153
 
BglI GCCn_nnn'nGGC

Cuts at:      0     889    2300    2524    2708    2784    3234 
   Size:        889    1411     224     184      76     450

  Fragments arranged by size: 

                1411     889     450     224     184      76
 
BglII A'GATC_T

Cuts at:      0     147     822     872    2567    3069    3234 
   Size:        147     675      50    1695     502     165

  Fragments arranged by size: 

                1695     675     502     165     147      50
 
Bme1580I G_kGCm'C

Cuts at:      0     631    1613    1756    3234 
   Size:        631     982     143    1478

  Fragments arranged by size: 

                1478     982     631     143
 
BmgBI CAC'GTC

Cuts at:      0    2027    3234 
   Size:       2027    1207
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     630     726    3234 
   Size:        630      96    2508

  Fragments arranged by size: 

                2508     630      96
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1518    1576    3234 
   Size:       1518      58    1658

  Fragments arranged by size: 

                1658    1518      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     795    1584    1971    2287    3013    3234 
   Size:        492     303     789     387     316     726     221

  Fragments arranged by size: 

                 789     726     492     387     316     303     221
 
Bpu10I CC'TnA_GC

Cuts at:      0     438     520     689    3234 
   Size:        438      82     169    2545

  Fragments arranged by size: 

                2545     438     169      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     847    2229    3234 
   Size:        847    1382    1005

  Fragments arranged by size: 

                1382    1005     847
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1207    3107    3234 
   Size:       1207    1900     127

  Fragments arranged by size: 

                1900    1207     127
 
BsaAI yAC'GTr

Cuts at:      0     936    2291    3234 
   Size:        936    1355     943

  Fragments arranged by size: 

                1355     943     936
 
BsaBI GATnn'nnATC

Cuts at:      0     146    2209    2518    2572    3234 
   Size:        146    2063     309      54     662

  Fragments arranged by size: 

                2063     662     309     146      54
 
BsaHI Gr'CG_yC

Cuts at:      0     443    3234 
   Size:        443    2791
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1771    1795    1801    1825    2287    2317    2971    3001 
   Size:       1771      24       6      24     462      30     654      30

Cuts at:   3001    3234 
   Size:        233

  Fragments arranged by size: 

                1771     654     462     233      30      30      24      24
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     495     508     894    1588    1948    1951    2623 
   Size:        250     245      13     386     694     360       3     672

Cuts at:   2623    2721    2724    3234 
   Size:         98       3     510

  Fragments arranged by size: 

                 694     672     510     386     360     250     245      98
                  13       3       3
 
BseYI C'CCAG_C

Cuts at:      0      20     252     276     468     970    1863    1890    2816 
   Size:         20     232      24     192     502     893      27     926

Cuts at:   2816    3153    3234 
   Size:        337      81

  Fragments arranged by size: 

                 926     893     502     337     232     192      81      27
                  24      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1367    2175    3234 
   Size:       1367     808    1059

  Fragments arranged by size: 

                1367    1059     808
 
BsiEI CG_ry'CG

Cuts at:      0     661    3234 
   Size:        661    2573
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     575    1949    2069    3234 
   Size:        401     174    1374     120    1165

  Fragments arranged by size: 

                1374    1165     401     174     120
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     450    2685    3234 
   Size:        450    2235     549

  Fragments arranged by size: 

                2235     549     450
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     110     401     476     575     631     833    1328 
   Size:         78      32     291      75      99      56     202     495

Cuts at:   1328    1613    1676    1756    1832    1949    2015    2069    2162 
   Size:        285      63      80      76     117      66      54      93

Cuts at:   2162    2305    3234 
   Size:        143     929

  Fragments arranged by size: 

                 929     495     291     285     202     143     117      99
                  93      80      78      76      75      66      63      56
                  54      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1315    1365    1416    1687    2174    2347    3234 
   Size:       1315      50      51     271     487     173     887

  Fragments arranged by size: 

                1315     887     487     271     173      51      50
 
BsrDI GCAATG_nn'

Cuts at:      0    1277    1793    3234 
   Size:       1277     516    1441

  Fragments arranged by size: 

                1441    1277     516
 
BsrFI r'CCGG_y

Cuts at:      0    2043    3234 
   Size:       2043    1191
 
BsrGI T'GTAC_A

Cuts at:      0    1183    3234 
   Size:       1183    2051
 
BssSI C'ACGA_G

Cuts at:      0    2296    3234 
   Size:       2296     938
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2078    2175    3234 
   Size:       2078      97    1059

  Fragments arranged by size: 

                2078    1059      97
 
BstEII G'GTnAC_C

Cuts at:      0    1428    1447    2916    3234 
   Size:       1428      19    1469     318

  Fragments arranged by size: 

                1469    1428     318      19
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     456     588     940    3234 
   Size:        456     132     352    2294

  Fragments arranged by size: 

                2294     456     352     132
 
BstYI r'GATC_y

Cuts at:      0     147     752     822     872     977    2126    2453    2567 
   Size:        147     605      70      50     105    1149     327     114

Cuts at:   2567    2719    3069    3234 
   Size:        152     350     165

  Fragments arranged by size: 

                1149     605     350     327     165     152     147     114
                 105      70      50
 
BtgI C'CryG_G

Cuts at:      0     195     449     587     785    2714    2957    3234 
   Size:        195     254     138     198    1929     243     277

  Fragments arranged by size: 

                1929     277     254     243     198     195     138
 
BtsI GCAGTG_nn'

Cuts at:      0     461     581    1212    3055    3234 
   Size:        461     120     631    1843     179

  Fragments arranged by size: 

                1843     631     461     179     120
 
EaeI y'GGCC_r

Cuts at:      0     658     788     881    3121    3234 
   Size:        658     130      93    2240     113

  Fragments arranged by size: 

                2240     658     130     113      93
 
EagI C'GGCC_G

Cuts at:      0     658    3234 
   Size:        658    2576
 
EarI CTCTTCn'nnn_

Cuts at:      0     481     743    1333    1567    1969    2587    3137    3234 
   Size:        481     262     590     234     402     618     550      97

  Fragments arranged by size: 

                 618     590     550     481     402     262     234      97
            
 
EcoICRI GAG'CTC

Cuts at:      0    1947    3234 
   Size:       1947    1287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     699     795     985    1021    1402    1584    1971 
   Size:        492     207      96     190      36     381     182     387

Cuts at:   1971    2287    2409    3013    3161    3234 
   Size:        316     122     604     148      73

  Fragments arranged by size: 

                 604     492     387     381     316     207     190     182
                 148     122      96      73      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     838    1003    1319    3234 
   Size:        838     165     316    1915

  Fragments arranged by size: 

                1915     838     316     165
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24    1096    1377    3100    3135    3234 
   Size:         24    1072     281    1723      35      99

  Fragments arranged by size: 

                1723    1072     281      99      35      24
 
EcoRI G'AATT_C

Cuts at:      0     994    2970    3228    3234 
   Size:        994    1976     258       6

  Fragments arranged by size: 

                1976     994     258       6
 
EcoRV GAT'ATC

Cuts at:      0     702    1247    3234 
   Size:        702     545    1987

  Fragments arranged by size: 

                1987     702     545
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2916    3234 
   Size:       2916     318
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171    1550    1582    1753    1785    1801    1833 
   Size:        139      32    1379      32     171      32      16      32

Cuts at:   1833    3234 
   Size:       1401

  Fragments arranged by size: 

                1401    1379     171     139      32      32      32      32
                  16
 
MfeI C'AATT_G

Cuts at:      0     632    3234 
   Size:        632    2602
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1197    1643    3234 
   Size:       1197     446    1591

  Fragments arranged by size: 

                1591    1197     446
 
MscI TGG'CCA

Cuts at:      0     883    3234 
   Size:        883    2351
 
MslI CAynn'nnrTG

Cuts at:      0     454     586    2086    2197    2215    3234 
   Size:        454     132    1500     111      18    1019

  Fragments arranged by size: 

                1500    1019     454     132     111      18
 
MspA1I CmG'CkG

Cuts at:      0     614     640    2805    2909    3045    3120    3234 
   Size:        614      26    2165     104     136      75     114

  Fragments arranged by size: 

                2165     614     136     114     104      75      26
 
NaeI GCC'GGC

Cuts at:      0    2045    3234 
   Size:       2045    1189
 
NcoI C'CATG_G

Cuts at:      0     587    2957    3234 
   Size:        587    2370     277

  Fragments arranged by size: 

                2370     587     277
 
NgoMIV G'CCGG_C

Cuts at:      0    2043    3234 
   Size:       2043    1191
 
NsiI A_TGCA'T

Cuts at:      0    2083    3234 
   Size:       2083    1151
 
NspI r_CATG'y

Cuts at:      0     763    2433    3234 
   Size:        763    1670     801

  Fragments arranged by size: 

                1670     801     763
 
PasI CC'CwG_GG

Cuts at:      0    2316    3234 
   Size:       2316     918
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     762    1498    2265    3234 
   Size:        762     736     767     969

  Fragments arranged by size: 

                 969     767     762     736
 
PfoI T'CCnGG_A

Cuts at:      0     989    2380    3080    3234 
   Size:        989    1391     700     154

  Fragments arranged by size: 

                1391     989     700     154
 
PmlI CAC'GTG

Cuts at:      0     936    2291    3234 
   Size:        936    1355     943

  Fragments arranged by size: 

                1355     943     936
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1771    1803    3234 
   Size:       1771      32    1431

  Fragments arranged by size: 

                1771    1431      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      24    1096    1377    3100    3135    3234 
   Size:         24    1072     281    1723      35      99

  Fragments arranged by size: 

                1723    1072     281      99      35      24
 
PstI C_TGCA'G

Cuts at:      0    1627    2171    3050    3234 
   Size:       1627     544     879     184

  Fragments arranged by size: 

                1627     879     544     184
 
PvuII CAG'CTG

Cuts at:      0     640    3234 
   Size:        640    2594
 
SacI G_AGCT'C

Cuts at:      0    1949    3234 
   Size:       1949    1285
 
SapI GCTCTTCn'nnn_

Cuts at:      0     481    1333    3234 
   Size:        481     852    1901

  Fragments arranged by size: 

                1901     852     481
 
ScaI AGT'ACT

Cuts at:      0     736     928    3234 
   Size:        736     192    2306

  Fragments arranged by size: 

                2306     736     192
 
SexAI A'CCwGG_T

Cuts at:      0    1451    3234 
   Size:       1451    1783
 
SfcI C'TryA_G

Cuts at:      0    1623    2167    3046    3234 
   Size:       1623     544     879     188

  Fragments arranged by size: 

                1623     879     544     188
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     889    3234 
   Size:        889    2345
 
SmaI CCC'GGG

Cuts at:      0    2656    3234 
   Size:       2656     578
 
SmlI C'TyrA_G

Cuts at:      0     826    2244    3234 
   Size:        826    1418     990

  Fragments arranged by size: 

                1418     990     826
 
SphI G_CATG'C

Cuts at:      0    2433    3234 
   Size:       2433     801
 
SrfI GCCC'GGGC

Cuts at:      0    2656    3234 
   Size:       2656     578
 
StuI AGG'CCT

Cuts at:      0     319     892    3234 
   Size:        319     573    2342

  Fragments arranged by size: 

                2342     573     319
 
StyI C'CwwG_G

Cuts at:      0      99     320     587    1043    1799    2703    2957    3234 
   Size:         99     221     267     456     756     904     254     277

  Fragments arranged by size: 

                 904     756     456     277     267     254     221      99
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     612    1882    1935    3097    3234 
   Size:        612    1270      53    1162     137

  Fragments arranged by size: 

                1270    1162     612     137      53
 
TatI w'GTAC_w

Cuts at:      0     734     926    1183    1259    3234 
   Size:        734     192     257      76    1975

  Fragments arranged by size: 

                1975     734     257     192      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2011    2043    2287    2319    2971    3003    3234 
   Size:       2011      32     244      32     652      32     231

  Fragments arranged by size: 

                2011     652     244     231      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273     798    1763    2047    3234 
   Size:        273     525     965     284    1187

  Fragments arranged by size: 

                1187     965     525     284     273
 
XmaI C'CCGG_G

Cuts at:      0    2654    3234 
   Size:       2654     580
 
XmnI GAAnn'nnTTC

Cuts at:      0     535    3234 
   Size:        535    2699
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI     AlfI     AloI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI     PstI    PvuII     SacI
     SapI     ScaI    SexAI     SfcI     SfiI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BclI
     BlpI     BmtI    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrBI
   BssHII    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     NarI     NdeI     NheI
     NotI     NruI     PacI     PciI     PmeI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI  Tth111I
     XbaI     XhoI     ZraI
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