Restriction map for pF1KE9522
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvrII       BamHI       BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HindIII     KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AocI         1799  CCTCAGG              CC^T[ACGT]AGG
AspI         2601  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1084  TGCGCA               TGC^GCA
BbeI         1244  GGCGCC               GGCGC^C
BbsI         1837  GTCTTC               GAAGAC(2/6)
BcgI         2053  GCACTTATCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          676  CTAG                 C^TAG
BpuAI        1837  GTCTTC               GAAGAC(2/6)
BseRI        2209  CTCCTC               GAGGAG(10/8)
Bsp1407I     2297  TGTACA               T^GTACA
BsrFI        1474  GCCGGC               [GA]^CCGG[CT]
BsrGI        2297  TGTACA               T^GTACA
BssSI         683  CTCGTG               CACGAG(-5/-1)
BstBI        1660  TTCGAA               TT^CGAA
BstYI         476  AGATCC               [GA]^GATC[CT]
Bsu36I       1799  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1474  GCCGGC               [GA]^CCGG[CT]
DraIII       2376  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          612  GACCTATGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1799  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1251  CCTCTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1084  TGCGCA               TGC^GCA
HincII       1237  GTTAAC               GT[CT]^[GA]AC
HindII       1237  GTTAAC               GT[CT]^[GA]AC
HpaI         1237  GTTAAC               GTT^AAC
KasI         1244  GGCGCC               G^GCGCC
MaeI          676  CTAG                 C^TAG
MflI          476  AGATCC               [GA]^GATC[CT]
MseI         1238  TTAA                 T^TAA
NaeI         1474  GCCGGC               GCC^GGC
NarI         1244  GGCGCC               GG^CGCC
NgoMIV       1474  GCCGGC               G^CCGGC
NspV         1660  TTCGAA               TT^CGAA
PflFI        2601  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1707  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         145  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1810  GAGCTC               GAGCT^C
SfoI         1244  GGCGCC               GGC^GCC
SfuI         1660  TTCGAA               TT^CGAA
SmlI          462  CTTGAG               C^T[CT][GA]AG
SspBI        2297  TGTACA               T^GTACA
TfiI          591  GATTC                G^A[AT]TC
Tru9I        1238  TTAA                 T^TAA
Tth111I      2601  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          782  CCAGTGCCCGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         476  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI         1039  GAATTC               [GA]^AATT[CT]
             1600  GAATTC              
Alw44I        118  GTGCAC               G^TGCAC
              254  GTGCAC              
AlwI          477  GATCC                GGATC(4/5)
                4  GGATC               
ApaLI         118  GTGCAC               G^TGCAC
              254  GTGCAC              
ApoI         1039  GAATTC               [GA]^AATT[CT]
             1600  GAATTC              
BsmI         1565  GCATTC               GAATGC(1/-1)
             2323  GCATTC              
BspMI         230  ACCTGC               ACCTGC(4/8)
             2305  ACCTGC              
BstAPI        228  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1565  GCATTCCGTGC         
EarI         2452  CTCTTC               CTCTTC(1/4)
             1527  GAAGAG              
EcoRI        1039  GAATTC               G^AATTC
             1600  GAATTC              
Ksp632I      2452  CTCTTC               CTCTTC(1/4)
             1527  GAAGAG              
NspI          288  ACATGC               [GA]CATG^[CT]
             1181  ACATGC              
PvuII        1301  CAGCTG               CAG^CTG
             2068  CAGCTG              
SmaI          824  CCCGGG               CCC^GGG
             1991  CCCGGG              
StuI          665  AGGCCT               AGG^CCT
             1796  AGGCCT              
Tsp509I      1040  AATT                 ^AATT
             1601  AATT                
XmaCI         824  CCCGGG               C^CCGGG
             1991  CCCGGG              
XmaI          824  CCCGGG               C^CCGGG
             1991  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
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