Restriction map for pF1KE9522
 (Linear) (Six-base) MAPSORT of: KIEE9522.seq  Check: 3607  from: 1  to: 2616



KIEE9522.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     239    2314    2616 
   Size:        239    2075     302

  Fragments arranged by size: 

                2075     302     239
 
AleI CACnn'nnGTG

Cuts at:      0     871    1786    1888    2616 
   Size:        871     915     102     728

  Fragments arranged by size: 

                 915     871     728     102
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1039    1071    2616 
   Size:       1039      32    1545

  Fragments arranged by size: 

                1545    1039      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     306     999    2616 
   Size:        183     123     693    1617

  Fragments arranged by size: 

                1617     693     183     123
 
ApaI G_GGCC'C

Cuts at:      0      61     897    1992    2616 
   Size:         61     836    1095     624

  Fragments arranged by size: 

                1095     836     624      61
 
ApaLI G'TGCA_C

Cuts at:      0     118     254    2616 
   Size:        118     136    2362

  Fragments arranged by size: 

                2362     136     118
 
ApoI r'AATT_y

Cuts at:      0    1039    1600    2616 
   Size:       1039     561    1016

  Fragments arranged by size: 

                1039    1016     561
 
AvaI C'yCGr_G

Cuts at:      0     824    1991    2061    2616 
   Size:        824    1167      70     555

  Fragments arranged by size: 

                1167     824     555      70
 
BanI G'GyrC_C

Cuts at:      0    1244    1732    1999    2103    2487    2616 
   Size:       1244     488     267     104     384     129

  Fragments arranged by size: 

                1244     488     384     267     129     104
 
BanII G_rGCy'C

Cuts at:      0      61     768     897    1508    1814    1992    2191    2616 
   Size:         61     707     129     611     306     178     199     425

  Fragments arranged by size: 

                 707     611     425     306     199     178     129      61
            
 
BbeI G_GCGC'C

Cuts at:      0    1248    2616 
   Size:       1248    1368
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1830    2616 
   Size:       1830     786
 
BbvCI CC'TCA_GC

Cuts at:      0      53     932    1470    2398    2616 
   Size:         53     879     538     928     218

  Fragments arranged by size: 

                 928     879     538     218      53
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2042    2076    2616 
   Size:       2042      34     540

  Fragments arranged by size: 

                2042     540      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     368     793    1652    2402    2504    2616 
   Size:        368     425     859     750     102     112

  Fragments arranged by size: 

                 859     750     425     368     112     102
 
Bme1580I G_kGCm'C

Cuts at:      0      61     122     258     690     789     897    1112    1194 
   Size:         61      61     136     432      99     108     215      82

Cuts at:   1194    1737    1921    1992    2004    2113    2257    2490    2616 
   Size:        543     184      71      12     109     144     233     126

  Fragments arranged by size: 

                 543     432     233     215     184     144     136     126
                 109     108      99      82      71      61      61      12
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109     776    1428    2550    2616 
   Size:        109     667     652    1122      66

  Fragments arranged by size: 

                1122     667     652     109      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     975    2616 
   Size:        975    1641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     205     316     919    1498    2334    2514    2616 
   Size:        103     102     111     603     579     836     180     102

  Fragments arranged by size: 

                 836     603     579     180     111     103     102     102
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      53     932    1470    2398    2616 
   Size:         53     879     538     928     218

  Fragments arranged by size: 

                 928     879     538     218      53
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     483    2616 
   Size:        483    2133
 
BsaHI Gr'CG_yC

Cuts at:      0     631    1245    1905    2616 
   Size:        631     614     660     711

  Fragments arranged by size: 

                 711     660     631     614
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      80     110     259     289     540     570     967     997 
   Size:         80      30     149      30     251      30     397      30

Cuts at:    997    1039    1069    2616 
   Size:         42      30    1547

  Fragments arranged by size: 

                1547     397     251     149      80      42      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2200    2616 
   Size:       2200     416
 
BseYI C'CCAG_C

Cuts at:      0      60      99     135    1299    1685    1755    1827    2003 
   Size:         60      39      36    1164     386      70      72     176

Cuts at:   2003    2616 
   Size:        613

  Fragments arranged by size: 

                1164     613     386     176      72      70      60      39
                  36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1400    1555    2208    2425    2616 
   Size:       1400     155     653     217     191

  Fragments arranged by size: 

                1400     653     217     191     155
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     167     258    1271    1814    2210    2616 
   Size:        122      45      91    1013     543     396     406

  Fragments arranged by size: 

                1013     543     406     396     122      91      45
 
BsmI GAATG_Cn'

Cuts at:      0    1565    2323    2616 
   Size:       1565     758     293

  Fragments arranged by size: 

                1565     758     293
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     122     167     258     690     768     789     897 
   Size:         61      61      45      91     432      78      21     108

Cuts at:    897    1112    1194    1271    1508    1737    1814    1921    1992 
   Size:        215      82      77     237     229      77     107      71

Cuts at:   1992    2004    2113    2191    2210    2257    2490    2616 
   Size:         12     109      78      19      47     233     126

  Fragments arranged by size: 

                 432     237     233     229     215     126     109     108
                 107      91      82      78      78      77      77      71
                  61      61      47      45      21      19      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239    2314    2616 
   Size:        239    2075     302

  Fragments arranged by size: 

                2075     302     239
 
BsrDI GCAATG_nn'

Cuts at:      0    1868    1967    2583    2616 
   Size:       1868      99     616      33

  Fragments arranged by size: 

                1868     616      99      33
 
BsrFI r'CCGG_y

Cuts at:      0    1474    2616 
   Size:       1474    1142
 
BsrGI T'GTAC_A

Cuts at:      0    2297    2616 
   Size:       2297     319
 
BssSI C'ACGA_G

Cuts at:      0     683    2616 
   Size:        683    1933
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     234    1571    2616 
   Size:        234    1337    1045

  Fragments arranged by size: 

                1337    1045     234
 
BstBI TT'CG_AA

Cuts at:      0    1661    2616 
   Size:       1661     955
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     450     582     954    1788    2049    2548    2616 
   Size:        450     132     372     834     261     499      68

  Fragments arranged by size: 

                 834     499     450     372     261     132      68
 
BstYI r'GATC_y

Cuts at:      0     476    2616 
   Size:        476    2140
 
Bsu36I CC'TnA_GG

Cuts at:      0    1800    2616 
   Size:       1800     816
 
BtgI C'CryG_G

Cuts at:      0     168     333     384     581    1160    1456    1883    2408 
   Size:        168     165      51     197     579     296     427     525

Cuts at:   2408    2616 
   Size:        208

  Fragments arranged by size: 

                 579     525     427     296     208     197     168     165
                  51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1240    1704    1706    2186    2199    2594    2616 
   Size:       1240     464       2     480      13     395      22

  Fragments arranged by size: 

                1240     480     464     395      22      13       2
 
BtsI GCAGTG_nn'

Cuts at:      0      25     398     924    1157    1392    1506    1895    1919 
   Size:         25     373     526     233     235     114     389      24

Cuts at:   1919    2152    2514    2616 
   Size:        233     362     102

  Fragments arranged by size: 

                 526     389     373     362     235     233     233     114
                 102      25      24
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1731    1766    2616 
   Size:       1731      35     850

  Fragments arranged by size: 

                1731     850      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2381    2616 
   Size:       2381     235
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     618    2616 
   Size:        618    1998
 
EaeI y'GGCC_r

Cuts at:      0     452     704     875    1231    1350    1739    2008    2039 
   Size:        452     252     171     356     119     389     269      31

Cuts at:   2039    2538    2580    2616 
   Size:        499      42      36

  Fragments arranged by size: 

                 499     452     389     356     269     252     171     119
                  42      36      31
 
EarI CTCTTCn'nnn_

Cuts at:      0    1522    2458    2616 
   Size:       1522     936     158

  Fragments arranged by size: 

                1522     936     158
 
EcoICRI GAG'CTC

Cuts at:      0    1812    2616 
   Size:       1812     804
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     205     316     919    1498    2334    2514    2616 
   Size:        103     102     111     603     579     836     180     102

  Fragments arranged by size: 

                 836     603     579     180     111     103     102     102
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1255    2616 
   Size:       1255    1361
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     893     894    1708    1988    2616 
   Size:         57     836       1     814     280     628

  Fragments arranged by size: 

                 836     814     628     280      57       1
 
EcoRI G'AATT_C

Cuts at:      0    1039    1600    2616 
   Size:       1039     561    1016

  Fragments arranged by size: 

                1039    1016     561
 
FspI TGC'GCA

Cuts at:      0    1086    2616 
   Size:       1086    1530
 
HaeII r_GCGC'y

Cuts at:      0     183     852    1248    1395    2616 
   Size:        183     669     396     147    1221

  Fragments arranged by size: 

                1221     669     396     183     147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     967     999    1108    1140    2616 
   Size:        259      32     676      32     109      32    1476

  Fragments arranged by size: 

                1476     676     259     109      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1239    2616 
   Size:       1239    1377
 
HpaI GTT'AAC

Cuts at:      0    1239    2616 
   Size:       1239    1377
 
KasI G'GCGC_C

Cuts at:      0    1244    2616 
   Size:       1244    1372
 
MscI TGG'CCA

Cuts at:      0     454     706     877    1741    2041    2540    2616 
   Size:        454     252     171     864     300     499      76

  Fragments arranged by size: 

                 864     499     454     300     252     171      76
 
MslI CAynn'nnrTG

Cuts at:      0     389     448     700     871    1138    1786    1888    2171 
   Size:        389      59     252     171     267     648     102     283

Cuts at:   2171    2377    2383    2616 
   Size:        206       6     233

  Fragments arranged by size: 

                 648     389     283     267     252     233     206     171
                 102      59       6
 
MspA1I CmG'CkG

Cuts at:      0    1061    1303    1685    2007    2070    2402    2616 
   Size:       1061     242     382     322      63     332     214

  Fragments arranged by size: 

                1061     382     332     322     242     214      63
 
NaeI GCC'GGC

Cuts at:      0    1476    2616 
   Size:       1476    1140
 
NarI GG'CG_CC

Cuts at:      0    1245    2616 
   Size:       1245    1371
 
NcoI C'CATG_G

Cuts at:      0     384     581    1160    1456    2616 
   Size:        384     197     579     296    1160

  Fragments arranged by size: 

                1160     579     384     296     197
 
NgoMIV G'CCGG_C

Cuts at:      0    1474    2616 
   Size:       1474    1142
 
NspI r_CATG'y

Cuts at:      0     292    1185    2616 
   Size:        292     893    1431

  Fragments arranged by size: 

                1431     893     292
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183     999    1410    2616 
   Size:        183     816     411    1206

  Fragments arranged by size: 

                1206     816     411     183
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1039    1071    2263    2295    2616 
   Size:       1039      32    1192      32     321

  Fragments arranged by size: 

                1192    1039     321      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1708    2616 
   Size:       1708     908
 
PshAI GACnn'nnGTC

Cuts at:      0     149    2616 
   Size:        149    2467
 
PspOMI G'GGCC_C

Cuts at:      0      57     893    1988    2616 
   Size:         57     836    1095     628

  Fragments arranged by size: 

                1095     836     628      57
 
PstI C_TGCA'G

Cuts at:      0     776    1066    1501    2616 
   Size:        776     290     435    1115

  Fragments arranged by size: 

                1115     776     435     290
 
PvuII CAG'CTG

Cuts at:      0    1303    2070    2616 
   Size:       1303     767     546

  Fragments arranged by size: 

                1303     767     546
 
SacI G_AGCT'C

Cuts at:      0    1814    2616 
   Size:       1814     802
 
SfcI C'TryA_G

Cuts at:      0     429     772    1062    1497    2616 
   Size:        429     343     290     435    1119

  Fragments arranged by size: 

                1119     435     429     343     290
 
SfoI GGC'GCC

Cuts at:      0    1246    2616 
   Size:       1246    1370
 
SmaI CCC'GGG

Cuts at:      0     826    1993    2616 
   Size:        826    1167     623

  Fragments arranged by size: 

                1167     826     623
 
SmlI C'TyrA_G

Cuts at:      0     462    2616 
   Size:        462    2154
 
StuI AGG'CCT

Cuts at:      0     667    1798    2616 
   Size:        667    1131     818

  Fragments arranged by size: 

                1131     818     667
 
StyI C'CwwG_G

Cuts at:      0     278     384     575     581    1006    1160    1456    2535 
   Size:        278     106     191       6     425     154     296    1079

Cuts at:   2535    2541    2616 
   Size:          6      75

  Fragments arranged by size: 

                1079     425     296     278     191     154     106      75
                   6       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      91     241     861    1324    1747    1819    2505    2616 
   Size:         91     150     620     463     423      72     686     111

  Fragments arranged by size: 

                 686     620     463     423     150     111      91      72
            
 
TatI w'GTAC_w

Cuts at:      0    1676    2297    2616 
   Size:       1676     621     319

  Fragments arranged by size: 

                1676     621     319
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112     540     572    2616 
   Size:         80      32     428      32    2044

  Fragments arranged by size: 

                2044     428      80      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2604    2616 
   Size:       2604      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     789    2616 
   Size:        789    1827
 
XmaI C'CCGG_G

Cuts at:      0     824    1991    2616 
   Size:        824    1167     625

  Fragments arranged by size: 

                1167     824     625
 

 Enzymes that do cut:

     AarI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
     HpaI     KasI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI    PflMI     PpiI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI   BssHII   BstEII  BstZ17I     ClaI     DraI     EagI
     EciI    EcoRV     FalI     FseI    FspAI  HindIII     KpnI     MfeI
     MluI     MmeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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