Restriction map for
pF1KE2349
(Linear) (Six-base) MAPSORT of: KIEE2349.seq Check: 3995 from: 1 to: 942
KIEE2349.orf
With 182 enzymes: SgfI *
May 20, 2011 15:42 ..
AccI GT'mk_AC
Cuts at: 0 191 942
Size: 191 751
Acc65I G'GTAC_C
Cuts at: 0 805 942
Size: 805 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 942
Size: 496 446
AleI CACnn'nnGTG
Cuts at: 0 122 268 374 942
Size: 122 146 106 568
Fragments arranged by size:
568 146 122 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 286 942
Size: 286 656
AlwNI CAG_nnn'CTG
Cuts at: 0 15 620 642 712 942
Size: 15 605 22 70 230
Fragments arranged by size:
605 230 70 22 15
ApaI G_GGCC'C
Cuts at: 0 29 942
Size: 29 913
AvaI C'yCGr_G
Cuts at: 0 21 942
Size: 21 921
AvrII C'CTAG_G
Cuts at: 0 534 942
Size: 534 408
BanI G'GyrC_C
Cuts at: 0 338 356 445 679 805 942
Size: 338 18 89 234 126 137
Fragments arranged by size:
338 234 137 126 89 18
BanII G_rGCy'C
Cuts at: 0 7 29 139 843 942
Size: 7 22 110 704 99
Fragments arranged by size:
704 110 99 22 7
BbeI G_GCGC'C
Cuts at: 0 342 942
Size: 342 600
BbvCI CC'TCA_GC
Cuts at: 0 590 942
Size: 590 352
BglI GCCn_nnn'nGGC
Cuts at: 0 24 942
Size: 24 918
BlpI GC'TnA_GC
Cuts at: 0 613 942
Size: 613 329
Bme1580I G_kGCm'C
Cuts at: 0 29 129 942
Size: 29 100 813
Fragments arranged by size:
813 100 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 880 942
Size: 880 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 505 557 806 857 942
Size: 151 354 52 249 51 85
Fragments arranged by size:
354 249 151 85 52 51
Bpu10I CC'TnA_GC
Cuts at: 0 590 942
Size: 590 352
BsaI GGTCTCn'nnnn_
Cuts at: 0 624 942
Size: 624 318
BsaHI Gr'CG_yC
Cuts at: 0 288 339 942
Size: 288 51 603
Fragments arranged by size:
603 288 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 184 186 214 942
Size: 156 28 2 28 728
Fragments arranged by size:
728 156 28 28 2
BseYI C'CCAG_C
Cuts at: 0 80 420 784 942
Size: 80 340 364 158
Fragments arranged by size:
364 340 158 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 942
Size: 737 205
BsiHKAI G_wGCw'C
Cuts at: 0 265 438 942
Size: 265 173 504
Fragments arranged by size:
504 265 173
Bsp1286I G_dGCh'C
Cuts at: 0 7 29 129 139 265 438 843 942
Size: 7 22 100 10 126 173 405 99
Fragments arranged by size:
405 173 126 100 99 22 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 942
Size: 326 616
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 942
Size: 15 927
BstEII G'GTnAC_C
Cuts at: 0 376 942
Size: 376 566
BstXI CCAn_nnnn'nTGG
Cuts at: 0 353 438 942
Size: 353 85 504
Fragments arranged by size:
504 353 85
BstYI r'GATC_y
Cuts at: 0 813 942
Size: 813 129
BtsI GCAGTG_nn'
Cuts at: 0 467 749 942
Size: 467 282 193
Fragments arranged by size:
467 282 193
DraIII CAC_nnn'GTG
Cuts at: 0 184 942
Size: 184 758
EaeI y'GGCC_r
Cuts at: 0 311 761 881 926 942
Size: 311 450 120 45 16
Fragments arranged by size:
450 311 120 45 16
EarI CTCTTCn'nnn_
Cuts at: 0 517 942
Size: 517 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 496 505 557 806 857 942
Size: 151 345 9 52 249 51 85
Fragments arranged by size:
345 249 151 85 52 51 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 714 942
Size: 714 228
EcoO109I rG'GnC_Cy
Cuts at: 0 12 25 480 639 831 942
Size: 12 13 455 159 192 111
Fragments arranged by size:
455 192 159 111 13 12
HaeII r_GCGC'y
Cuts at: 0 342 942
Size: 342 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 240 272 942
Size: 184 32 24 32 670
Fragments arranged by size:
670 184 32 32 24
KasI G'GCGC_C
Cuts at: 0 338 942
Size: 338 604
KpnI G_GTAC'C
Cuts at: 0 809 942
Size: 809 133
MscI TGG'CCA
Cuts at: 0 313 763 883 928 942
Size: 313 450 120 45 14
Fragments arranged by size:
450 313 120 45 14
MslI CAynn'nnrTG
Cuts at: 0 122 268 374 942
Size: 122 146 106 568
Fragments arranged by size:
568 146 122 106
MspA1I CmG'CkG
Cuts at: 0 268 942
Size: 268 674
NarI GG'CG_CC
Cuts at: 0 339 942
Size: 339 603
PpuMI rG'GwC_Cy
Cuts at: 0 831 942
Size: 831 111
PspOMI G'GGCC_C
Cuts at: 0 25 942
Size: 25 917
PstI C_TGCA'G
Cuts at: 0 232 240 942
Size: 232 8 702
Fragments arranged by size:
702 232 8
PvuII CAG'CTG
Cuts at: 0 268 942
Size: 268 674
SapI GCTCTTCn'nnn_
Cuts at: 0 517 942
Size: 517 425
SfcI C'TryA_G
Cuts at: 0 228 236 528 942
Size: 228 8 292 414
Fragments arranged by size:
414 292 228 8
SfoI GGC'GCC
Cuts at: 0 340 942
Size: 340 602
SmaI CCC'GGG
Cuts at: 0 23 942
Size: 23 919
SpeI A'CTAG_T
Cuts at: 0 778 942
Size: 778 164
StuI AGG'CCT
Cuts at: 0 226 672 942
Size: 226 446 270
Fragments arranged by size:
446 270 226
StyI C'CwwG_G
Cuts at: 0 86 517 534 789 809 893 942
Size: 86 431 17 255 20 84 49
Fragments arranged by size:
431 255 86 84 49 20 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 942
Size: 642 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 942
Size: 156 32 754
Fragments arranged by size:
754 156 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 891 942
Size: 756 135 51
Fragments arranged by size:
756 135 51
XmaI C'CCGG_G
Cuts at: 0 21 942
Size: 21 921
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaI AvaI
AvrII BanI BanII BbeI BbvCI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspMI BstAPI BstEII BstXI BstYI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI KpnI MscI MslI MspA1I NarI PpuMI PspOMI
PstI PvuII SapI SfcI SfoI SmaI SpeI StuI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI