Restriction map for pF1KE3426
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AccI        AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BsaAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MaeI        MfeI        MluI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII          88  GACGTC               GACGT^C
Acc65I        305  GGTACC               G^GTACC
AccIII        393  TCCGGA               T^CCGGA
AcsI          514  AAATTT               [GA]^AATT[CT]
ApoI          514  AAATTT               [GA]^AATT[CT]
Asp718I       305  GGTACC               G^GTACC
AvaI         1642  CTCGAG               C^[CT]CG[GA]G
BanII        1062  GAGCTC               G[GA]GC[CT]^C
BglI         1731  GCCTGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         110  AGATCT               A^GATCT
BpmI         1633  CTGGAG               CTGGAG(16/14)
BsaBI         203  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         393  TCCGGA               T^CCGGA
BsiEI        1182  CGGCCG               CG[GA][CT]^CG
BsiWI          65  CGTACG               C^GTACG
BsoBI        1642  CTCGAG               C^[CT]CG[GA]G
BspEI         393  TCCGGA               T^CCGGA
BspHI          92  TCATGA               T^CATGA
BsrBI        1735  GAGCGG               CCGCTC(-3/-3)
BsrDI         449  GCAATG               GCAATG(2/0)
BssSI         533  CTCGTG               CACGAG(-5/-1)
BstXI         179  CCAGCATAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         110  AGATCT               [GA]^GATC[CT]
DrdI          207  GACATCTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1182  CGGCCG               C^GGCCG
EclXI        1182  CGGCCG               C^GGCCG
Eco52I       1182  CGGCCG               C^GGCCG
KpnI          305  GGTACC               GGTAC^C
MamI          203  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          110  AGATCT               [GA]^GATC[CT]
MroI          393  TCCGGA               T^CCGGA
NspI          668  ACATGC               [GA]CATG^[CT]
PaeR7I       1642  CTCGAG               C^TCGAG
PshAI        1515  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI           92  TCATGA               T^CATGA
SacI         1062  GAGCTC               GAGCT^C
SapI         1335  GCTCTTC              GCTCTTC(1/4)
SexAI         366  ACCAGGT              A^CC[AT]GGT
SfiI         1730  GGCCTGAGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI         1643  TCGA                 T^CGA
Tsp509I       515  AATT                 ^AATT
TthHB8I      1643  TCGA                 T^CGA
XhoI         1642  CTCGAG               C^TCGAG
XhoII         110  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI           14  GGATC                GGATC(4/5)
             1082  GGATC               
BbvCI        1551  CCTCAGC              CCTCAGC(-5/-2)
             1594  CCTCAGC             
BlpI           54  GCTCAGC              GC^T[ACGT]AGC
              757  GCTGAGC             
Bpu1102I       54  GCTCAGC              GC^T[ACGT]AGC
              757  GCTGAGC             
BsaWI         393  TCCGGA               [AT]^CCGG[AT]
             1208  ACCGGA              
BssHII        696  GCGCGC               G^CGCGC
              783  GCGCGC              
CelII          54  GCTCAGC              GC^T[ACGT]AGC
              757  GCTGAGC             
DraII         161  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1621  GGGACCC             
DraIII        365  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1044  CACCGGGTG           
EcoO109I      161  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1621  GGGACCC             
MaeII          89  ACGT                 A^CGT
              487  ACGT                
NaeI          826  GCCGGC               GCC^GGC
             1180  GCCGGC              
NcoI         1493  CCATGG               C^CATGG
             1661  CCATGG              
NgoMIV        826  GCCGGC               G^CCGGC
             1180  GCCGGC              
PpuMI         161  AGGTCCT              [GA]G^G[AT]CC[CT]
             1621  GGGACCC             
PstI          166  CTGCAG               CTGCA^G
              840  CTGCAG              
PvuII         456  CAGCTG               CAG^CTG
             1597  CAGCTG              
StuI         1707  AGGCCT               AGG^CCT
             1721  AGGCCT              
TfiI          190  GAATC                G^A[AT]TC
              894  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaII       BalI        BanI        BbeI        
BbsI        BbvI        BcnI        BmyI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoNI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        StyI        TseI        Tsp45I      
TspRI       
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