Restriction map for
pF1KE3426
(Circular) (Six-base) MAPSORT of: pF1KE3426.seq Check: 7335 from: 1 to: 4892
pF1KE3426 4892 bp
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1773 1860 1773
Size: 87 4805
AatII G_ACGT'C
Cuts at: 200 200
Size: 4892
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 413 1908 413
Size: 1495 3397
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3149 3896 4164 2717
Size: 432 747 268 3445
Fragments arranged by size:
3445 747 432 268
AflIII A'CryG_T
Cuts at: 2244 3348 4325 2244
Size: 1104 977 2811
Fragments arranged by size:
2811 1104 977
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AleI CACnn'nnGTG
Cuts at: 1339 1616 1618 1339
Size: 277 2 4613
Fragments arranged by size:
4613 277 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 1777 1809 206
Size: 32 1539 32 3289
Fragments arranged by size:
3289 1539 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2197 3764 2197
Size: 1567 3325
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 622 1896 4245 4501 622
Size: 1274 2349 256 1013
Fragments arranged by size:
2349 1274 1013 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1750 1912 1750
Size: 162 4730
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 396 413 422 471 576 847 906 1200 1908
Size: 17 9 49 105 271 59 294 708
Cuts at: 1908 2322 2570 2605 396
Size: 414 248 35 2683
Fragments arranged by size:
2683 708 414 294 271 248 105 59
49 35 17 9
BanII G_rGCy'C
Cuts at: 1174 1906 2936 1174
Size: 732 1030 3130
Fragments arranged by size:
3130 1030 732
BbeI G_GCGC'C
Cuts at: 426 851 910 2326 2574 426
Size: 425 59 1416 248 2744
Fragments arranged by size:
2744 1416 425 248 59
BbsI GAAGACnn'nnnn_
Cuts at: 1077 1101 1368 1077
Size: 24 267 4601
Fragments arranged by size:
4601 267 24
BbvCI CC'TCA_GC
Cuts at: 1660 1703 1660
Size: 43 4849
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3551 4892 2790
Size: 761 1341 2790
Fragments arranged by size:
2790 1341 761
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 1845 1845
Size: 4892
BglII A'GATC_T
Cuts at: 218 2407 4444 218
Size: 2189 2037 666
Fragments arranged by size:
2189 2037 666
BlpI GC'TnA_GC
Cuts at: 163 866 2004 163
Size: 703 1138 3051
Fragments arranged by size:
3051 1138 703
Bme1580I G_kGCm'C
Cuts at: 474 497 587 1205 2517 2610 3666 474
Size: 23 90 618 1312 93 1056 1700
Fragments arranged by size:
1700 1312 1056 618 93 90 23
BmgBI CAC'GTC
Cuts at: 596 596
Size: 4892
BmrI ACTGGGnnnn_n'
Cuts at: 240 2104 2185 2520 4664 240
Size: 1864 81 335 2144 468
Fragments arranged by size:
2144 1864 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1848 3259 1848
Size: 1411 3481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 1762
Size: 4892
Bpu10I CC'TnA_GC
Cuts at: 239 1660 1703 1812 1841 239
Size: 1421 43 109 29 3290
Fragments arranged by size:
3290 1421 109 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1188 2068 2947 3439 3737 247
Size: 941 880 879 492 298 1402
Fragments arranged by size:
1402 941 880 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1273 1407 1738 1747 4596 1273
Size: 134 331 9 2849 1569
Fragments arranged by size:
2849 1569 331 134 9
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 315 2406 4443 6
Size: 309 2091 2037 455
Fragments arranged by size:
2091 2037 455 309
BsaHI Gr'CG_yC
Cuts at: 197 423 706 848 907 1361 2323 2571 3273
Size: 226 283 142 59 454 962 248 702
Cuts at: 3273 4769 197
Size: 1496 320
Fragments arranged by size:
1496 962 702 454 320 283 248 226
142 59
BsaWI w'CCGG_w
Cuts at: 501 1316 2081 2602 3280 3554 3701 4112 501
Size: 815 765 521 678 274 147 411 1281
Fragments arranged by size:
1281 815 765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1156 1186 1003
Size: 30 123 30 4709
Fragments arranged by size:
4709 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 895 898 1840 397
Size: 498 3 942 3449
Fragments arranged by size:
3449 942 498 3
BseYI C'CCAG_C
Cuts at: 465 566 1091 1160 1734 2317 3075 3652 4148
Size: 101 525 69 574 583 758 577 496
Cuts at: 4148 465
Size: 1209
Fragments arranged by size:
1209 758 583 577 574 525 496 101
69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 736 741 1009 214
Size: 522 5 268 4097
Fragments arranged by size:
4097 522 268 5
BsiEI CG_ry'CG
Cuts at: 104 1293 2434 2480 3688 104
Size: 1189 1141 46 1208 1308
Fragments arranged by size:
1308 1208 1189 1141 46
BsiHKAI G_wGCw'C
Cuts at: 164 240 728 839 1174 1226 1262 1346 1906
Size: 76 488 111 335 52 36 84 560
Cuts at: 1906 2684 2874 3666 164
Size: 778 190 792 1390
Fragments arranged by size:
1390 792 778 560 488 335 190 111
84 76 52 36
BsiWI C'GTAC_G
Cuts at: 173 173
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 164 240 474 497 587 728 839 1174 1205
Size: 76 234 23 90 141 111 335 31
Cuts at: 1205 1226 1262 1346 1906 2517 2610 2684 2874
Size: 21 36 84 560 611 93 74 190
Cuts at: 2874 2936 3666 164
Size: 62 730 1390
Fragments arranged by size:
1390 730 611 560 335 234 190 141
111 93 90 84 76 74 62 36
31 23 21
BspEI T'CCGG_A
Cuts at: 501 4112 501
Size: 3611 1281
BspHI T'CATG_A
Cuts at: 200 4068 200
Size: 3868 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1031 1773 1860 1942 2156 2458 2839 401
Size: 630 742 87 82 214 302 381 2454
Fragments arranged by size:
2454 742 630 381 302 214 87 82
BsrBI CCG'CTC
Cuts at: 50 1845 3184 3238 4722 4887 50
Size: 1795 1339 54 1484 165 55
Fragments arranged by size:
1795 1484 1339 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 2804 564
Size: 2240 2652
BsrFI r'CCGG_y
Cuts at: 934 1288 1292 2890 3071 3280 934
Size: 354 4 1598 181 209 2546
Fragments arranged by size:
2546 1598 354 209 181 4
BssHII G'CGCG_C
Cuts at: 804 891 804
Size: 87 4805
BssSI C'ACGA_G
Cuts at: 641 3163 3521 641
Size: 2522 358 2012
Fragments arranged by size:
2522 2012 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 294 294
Size: 4892
BstYI r'GATC_y
Cuts at: 1 218 1917 2384 2407 2742 2988 4000 4086
Size: 217 1699 467 23 335 246 1012 86
Cuts at: 4086 4098 4444 1
Size: 12 346 449
Fragments arranged by size:
1699 1012 467 449 346 335 246 217
86 23 12
BtgI C'CryG_G
Cuts at: 107 683 1601 1769 107
Size: 576 918 168 3230
Fragments arranged by size:
3230 918 576 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 882 2842 3023 3186 882
Size: 1960 181 163 2588
Fragments arranged by size:
2588 1960 181 163
BtsI GCAGTG_nn'
Cuts at: 753 1679 2135 753
Size: 926 456 3510
Fragments arranged by size:
3510 926 456
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DraIII CAC_nnn'GTG
Cuts at: 478 1157 478
Size: 679 4213
DrdI GACnn_nn'nnGTC
Cuts at: 321 2598 3456 321
Size: 2277 858 1757
Fragments arranged by size:
2277 1757 858
EaeI y'GGCC_r
Cuts at: 460 983 1145 1290 1382 1695 1713 1846 2477
Size: 523 162 145 92 313 18 133 631
Cuts at: 2477 2651 3042 3069 4414 460
Size: 174 391 27 1345 938
Fragments arranged by size:
1345 938 631 523 391 313 174 162
145 133 92 27 18
EagI C'GGCC_G
Cuts at: 1290 2477 1290
Size: 1187 3705
EarI CTCTTCn'nnn_
Cuts at: 1243 1450 1862 2915 3125 1243
Size: 207 412 1053 210 3010
Fragments arranged by size:
3010 1053 412 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3100 3410 3556 4702 3100
Size: 310 146 1146 3290
Fragments arranged by size:
3290 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1904 1172
Size: 732 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2717 3149 3896 4164 1762
Size: 955 432 747 268 2490
Fragments arranged by size:
2490 955 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 741 877 1822 1861 741
Size: 136 945 39 3772
Fragments arranged by size:
3772 945 136 39
EcoO109I rG'GnC_Cy
Cuts at: 270 1730 2031 270
Size: 1460 301 3131
Fragments arranged by size:
3131 1460 301
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4892
HaeII r_GCGC'y
Cuts at: 142 426 851 873 910 2326 2574 3596 4575
Size: 284 425 22 37 1416 248 1022 979
Cuts at: 4575 142
Size: 459
Fragments arranged by size:
1416 1022 979 459 425 284 248 37
22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 340 372 1156 1188 1454 1486 2734 2764 2766
Size: 32 784 32 266 32 1248 30 2
Cuts at: 2766 2796 340
Size: 30 2436
Fragments arranged by size:
2436 1248 784 266 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4892
KasI G'GCGC_C
Cuts at: 422 847 906 2322 2570 422
Size: 425 59 1416 248 2744
Fragments arranged by size:
2744 1416 425 248 59
KpnI G_GTAC'C
Cuts at: 417 1912 417
Size: 1495 3397
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3563 3747 3563
Size: 184 4708
MscI TGG'CCA
Cuts at: 985 1147 1384 1715 2653 985
Size: 162 237 331 938 3224
Fragments arranged by size:
3224 938 331 237 162
MslI CAynn'nnrTG
Cuts at: 775 1039 1198 1339 1616 1618 2881 3008 775
Size: 264 159 141 277 2 1263 127 2659
Fragments arranged by size:
2659 1263 277 264 159 141 127 2
MspA1I CmG'CkG
Cuts at: 465 566 931 1105 1535 1574 1707 2003 2317
Size: 101 365 174 430 39 133 296 314
Cuts at: 2317 2677 3690 3935 465
Size: 360 1013 245 1422
Fragments arranged by size:
1422 1013 430 365 360 314 296 245
174 133 101 39
NaeI GCC'GGC
Cuts at: 936 1290 3073 936
Size: 354 1783 2755
Fragments arranged by size:
2755 1783 354
NarI GG'CG_CC
Cuts at: 423 848 907 2323 2571 423
Size: 425 59 1416 248 2744
Fragments arranged by size:
2744 1416 425 248 59
NcoI C'CATG_G
Cuts at: 107 1601 1769 107
Size: 1494 168 3230
Fragments arranged by size:
3230 1494 168
NgoMIV G'CCGG_C
Cuts at: 934 1288 3071 934
Size: 354 1783 2755
Fragments arranged by size:
2755 1783 354
NspI r_CATG'y
Cuts at: 780 1945 2445 3352 780
Size: 1165 500 907 2320
Fragments arranged by size:
2320 1165 907 500
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PfoI T'CCnGG_A
Cuts at: 1174 1174
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4860
PpuMI rG'GwC_Cy
Cuts at: 270 1730 270
Size: 1460 3432
PshAI GACnn'nnGTC
Cuts at: 1627 1627
Size: 4892
PstI C_TGCA'G
Cuts at: 278 952 1939 2624 278
Size: 674 987 685 2546
Fragments arranged by size:
2546 987 685 674
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 566 1707 2317 2677 566
Size: 1141 610 360 2781
Fragments arranged by size:
2781 1141 610 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 1174 1906 1174
Size: 732 4160
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SanDI GG'GwC_CC
Cuts at: 1730 1730
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 1450 2915 3125 1450
Size: 1465 210 3217
Fragments arranged by size:
3217 1465 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SexAI A'CCwGG_T
Cuts at: 474 474
Size: 4892
SfcI C'TryA_G
Cuts at: 33 274 606 948 1587 1861 1935 2620 3613
Size: 241 332 342 639 274 74 685 993
Cuts at: 3613 3804 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 639 342 332 274 241
191 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 1845 1845
Size: 4892
SfoI GGC'GCC
Cuts at: 424 849 908 2324 2572 424
Size: 425 59 1416 248 2744
Fragments arranged by size:
2744 1416 425 248 59
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 262 1167 1750 2047 2962 3454 3716 262
Size: 905 583 297 915 492 262 1438
Fragments arranged by size:
1438 915 905 583 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
StuI AGG'CCT
Cuts at: 1817 1831 1817
Size: 14 4878
StyI C'CwwG_G
Cuts at: 107 1601 1769 1781 2026 2380 107
Size: 1494 168 12 245 354 2619
Fragments arranged by size:
2619 1494 354 245 168 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 2475 2608 3067 3276 414
Size: 2061 133 459 209 2030
Fragments arranged by size:
2061 2030 459 209 133
TatI w'GTAC_w
Cuts at: 4407 4407
Size: 4892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 4860
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2387 2387
Size: 4892
XhoI C'TCGA_G
Cuts at: 1750 1750
Size: 4892
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
ZraI GAC'GTC
Cuts at: 198 198
Size: 4892
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI
BcgI BfrBI BmtI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PsiI PspOMI PspXI PsrI SacII SgrAI
SnaBI SrfI SspI SwaI XmnI