Restriction map for pF1KE2310
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AvrII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BspEI       BspLU11I    BsrBI       BssHII      BstBI       
CelII       CpoI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       FseI        HaeII       
HgaI        Hin1I       HpaI        KasI        KspI        
MluI        MroI        NarI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RsrII       SacII       
SalI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        StuI        SwaI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

Acc65I       2211  GGTACC               G^GTACC
AccII        2777  CGCG                 CG^CG
AclI         2810  AACGTT               AA^CGTT
AflIII        257  ACGTGT               A^C[GA][CT]GT
Asp700I       453  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2211  GGTACC               G^GTACC
AspI         1712  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2933  CTCGAG               C^[CT]CG[GA]G
AviII         146  TGCGCA               TGC^GCA
BalI         1219  TGGCCA               TGG^CCA
BamHI        3818  GGATCC               G^GATCC
BclI         2309  TGATCA               T^GATCA
BsaAI         256  TACGTG               [CT]AC^GT[GA]
BsaBI        1825  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          859  GCATTC               GAATGC(1/-1)
BsoBI        2933  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1729  TGTACA               T^GTACA
BspDI         338  ATCGAT               AT^CGAT
BspHI        1890  TCATGA               T^CATGA
BsrDI         610  CATTGC               GCAATG(2/0)
BsrFI           6  GCCGGC               [GA]^CCGG[CT]
BsrGI        1729  TGTACA               T^GTACA
Bst1107I     2672  GTATAC               GTA^TAC
BstAPI       3280  GCACAGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1260  GGTGACC              G^GT[ACGT]ACC
BstPI        1260  GGTGACC              G^GT[ACGT]ACC
BstUI        2777  CGCG                 CG^CG
BstZ17I      2672  GTATAC               GTA^TAC
Cfr10I          6  GCCGGC               [GA]^CCGG[CT]
ClaI          338  ATCGAT               AT^CGAT
DraIII       3281  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          525  GACTCTTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1260  GGTGACC              G^GT[ACGT]ACC
EcoRI        2768  GAATTC               G^AATTC
EcoT22I      3569  ATGCAT               ATGCA^T
FauI           49  GCGGG                CCCGC(4/6)
FbaI         2309  TGATCA               T^GATCA
FspI          146  TGCGCA               TGC^GCA
HincII        534  GTCAAC               GT[CT]^[GA]AC
HindII        534  GTCAAC               GT[CT]^[GA]AC
KpnI         2211  GGTACC               GGTAC^C
MamI         1825  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1139  CAATTG               C^AATTG
MluNI        1219  TGGCCA               TGG^CCA
MscI         1219  TGGCCA               TGG^CCA
MunI         1139  CAATTG               C^AATTG
MvnI         2777  CGCG                 CG^CG
NaeI            6  GCCGGC               GCC^GGC
NdeI          851  CATATG               CA^TATG
NgoMIV          6  GCCGGC               G^CCGGC
NheI         2589  GCTAGC               G^CTAGC
NsiI         3569  ATGCAT               ATGCA^T
PaeR7I       2933  CTCGAG               C^TCGAG
PflFI        1712  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1552  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2810  AACGTT               AA^CGTT
PstI         3775  CTGCAG               CTGCA^G
RcaI         1890  TCATGA               T^CATGA
SacI          108  GAGCTC               GAGCT^C
SbfI         3774  CCTGCAGG             CCTGCA^GG
SexAI        1256  ACCTGGT              A^CC[AT]GGT
SpeI         1885  ACTAGT               A^CTAGT
Sse8387I     3774  CCTGCAGG             CCTGCA^GG
SspBI        1729  TGTACA               T^GTACA
Tth111I      1712  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2933  CTCGAG               C^TCGAG
XmnI          453  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          721  CCTAAGG              CC^T[ACGT]AGG
             3073  CCTCAGG             
BbvCI        2818  CCTCAGC              CCTCAGC(-5/-2)
             3055  GCTGAGG             
BsaI         2936  GAGACC               GGTCTC(1/5)
             3533  GAGACC              
BsaWI        2342  TCCGGT               [AT]^CCGG[AT]
             3908  ACCGGA              
BspMI         287  ACCTGC               ACCTGC(4/8)
             3773  ACCTGC              
BstYI        1589  GGATCT               [GA]^GATC[CT]
             3818  GGATCC              
Bsu36I        721  CCTAAGG              CC^T[ACGT]AGG
             3073  CCTCAGG             
CfoI          147  GCGC                 GCG^C
             1361  GCGC                
DraI          360  TTTAAA               TTT^AAA
             1747  TTTAAA              
EaeI          582  TGGCCG               [CT]^GGCC[GA]
             1219  TGGCCA              
Eco81I        721  CCTAAGG              CC^T[ACGT]AGG
             3073  CCTCAGG             
HhaI          147  GCGC                 GCG^C
             1361  GCGC                
HinP1I        147  GCGC                 G^CGC
             1361  GCGC                
HindIII       995  AAGCTT               A^AGCTT
             2980  AAGCTT              
MflI         1589  GGATCT               [GA]^GATC[CT]
             3818  GGATCC              
MspA1I       1961  CAGCTG               C[AC]G^C[GT]G
             2377  CAGCTG              
PflMI         479  CCATTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1412  CCATTAACTGG         
PvuII        1961  CAGCTG               CAG^CTG
             2377  CAGCTG              
SapI         2031  GAAGAGC              GCTCTTC(1/4)
             1980  GCTCTTC             
SspI          354  AATATT               AAT^ATT
             3794  AATATT              
Van91I        479  CCATTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1412  CCATTAACTGG         
XcmI          812  CCACCTTTCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2276  CCAAGGCAAATGTGG     
XhoII        1589  GGATCT               [GA]^GATC[CT]
             3818  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApoI        AspEI       
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BssSI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
Eam1105I    EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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