Restriction map for pF1KB6904
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BbeI        BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AspHI         394  GAGCTC               G[AT]GC[AT]^C
BalI          140  TGGCCA               TGG^CCA
BamHI         494  GGATCC               G^GATCC
BanI          313  GGCACC               G^G[CT][GA]CC
BanII         394  GAGCTC               G[GA]GC[CT]^C
BbrPI         115  CACGTG               CAC^GTG
BbsI           97  GTCTTC               GAAGAC(2/6)
BcgI          505  GCAGTTAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          207  CCGGG                CC^[GC]GG
BfaI          292  CTAG                 C^TAG
BlpI           31  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       31  GCTCAGC              GC^T[ACGT]AGC
BpuAI          97  GTCTTC               GAAGAC(2/6)
BsaAI         115  CACGTG               [CT]AC^GT[GA]
BsiHKAI       394  GAGCTC               G[AT]GC[AT]^C
BsiYI          50  CCAGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           50  CCAGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         372  GGGAC                GGGAC(10/14)
BsrDI         109  GCAATG               GCAATG(2/0)
BstF5I         90  CATCC                GGATG(2/0)
BstYI         494  GGATCC               [GA]^GATC[CT]
CelII          31  GCTCAGC              GC^T[ACGT]AGC
DraII          52  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          362  CCATGG               C^C[GA][CT]GG
EaeI          140  TGGCCA               [CT]^GGCC[GA]
EarI           85  CTCTTC               CTCTTC(1/4)
EcoO109I       52  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       179  ATGCAT               ATGCA^T
FokI           90  CATCC                GGATG(9/13)
HapII         207  CCGG                 C^CGG
HincII        282  GTTGAC               GT[CT]^[GA]AC
HindII        282  GTTGAC               GT[CT]^[GA]AC
HpaII         207  CCGG                 C^CGG
Ksp632I        85  CTCTTC               CTCTTC(1/4)
MaeI          292  CTAG                 C^TAG
MaeII         116  ACGT                 A^CGT
MflI          494  GGATCC               [GA]^GATC[CT]
MluNI         140  TGGCCA               TGG^CCA
MscI          140  TGGCCA               TGG^CCA
MspI          207  CCGG                 C^CGG
NciI          207  CCGGG                CC^[GC]GG
NcoI          362  CCATGG               C^CATGG
NsiI          179  ATGCAT               ATGCA^T
PflMI          50  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          374  GACTC                GAGTC(4/5)
PmaCI         115  CACGTG               CAC^GTG
PmlI          115  CACGTG               CAC^GTG
PpuMI          52  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         243  GACTGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          394  GAGCTC               GAGCT^C
Van91I         50  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         494  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          136  GTAC                 GT^AC
              402  GTAC                
AlwNI          51  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              473  CAGATTCTG           
AvaII          53  GGACC                G^G[AT]CC
              204  GGTCC               
BpmI          185  CTCCAG               CTGGAG(16/14)
              318  CTCCAG              
BseRI         487  CTCCTC               GAGGAG(10/8)
              538  CTCCTC              
BsgI           63  CTGCAC               GTGCAG(16/14)
              306  CTGCAC              
BsrI          138  ACTGG                ACTGG(1/-1)
              320  CCAGT               
DpnII         457  GATC                 ^GATC
              495  GATC                
HphI          210  GGTGA                GGTGA(8/7)
              240  GGTGA               
MaeIII        211  GTGAC                ^GT[ACGT]AC
              241  GTGAC               
MboI          457  GATC                 ^GATC
              495  GATC                
NdeII         457  GATC                 ^GATC
              495  GATC                
PvuII          34  CAGCTG               CAG^CTG
              545  CAGCTG              
RsaI          136  GTAC                 GT^AC
              402  GTAC                
Sau3AI        457  GATC                 ^GATC
              495  GATC                
SfaNI         155  GCATC                GCATC(5/9)
              181  GCATC               
TfiI          298  GATTC                G^A[AT]TC
              475  GATTC               
Tsp45I        211  GTGAC                ^GT[GC]AC
              241  GTGAC               
Tsp509I       453  AATT                 ^AATT
              641  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BmyI        
BsaJI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      EcoT14I     Fnu4HI      
HaeIII      HinfI       ItaI        MboII       MnlI        
MspA1I      MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       StyI        TseI        TspRI       
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