Restriction map for pF1KB6904
 (Linear) (Six-base) MAPSORT of: KIBB6904.seq  Check: 26  from: 1  to: 669



KIBB6904.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     669 
   Size:        266     403
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     127     669 
   Size:        127     542
 
AlwNI CAG_nnn'CTG

Cuts at:      0      56     478     669 
   Size:         56     422     191

  Fragments arranged by size: 

                 422     191      56
 
BamHI G'GATC_C

Cuts at:      0     494     669 
   Size:        494     175
 
BanI G'GyrC_C

Cuts at:      0     313     669 
   Size:        313     356
 
BanII G_rGCy'C

Cuts at:      0     398     669 
   Size:        398     271
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90     669 
   Size:         90     579
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     494     528     669 
   Size:        494      34     141

  Fragments arranged by size: 

                 494     141      34
 
BfrBI ATG'CAT

Cuts at:      0     181     669 
   Size:        181     488
 
BlpI GC'TnA_GC

Cuts at:      0      32     669 
   Size:         32     637
 
Bme1580I G_kGCm'C

Cuts at:      0     316     472     669 
   Size:        316     156     197

  Fragments arranged by size: 

                 316     197     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     303     669 
   Size:        170     133     366

  Fragments arranged by size: 

                 366     170     133
 
BsaAI yAC'GTr

Cuts at:      0     117     669 
   Size:        117     552
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     385     415     669 
   Size:        385      30     254

  Fragments arranged by size: 

                 385     254      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478     529     669 
   Size:        478      51     140

  Fragments arranged by size: 

                 478     140      51
 
BseYI C'CCAG_C

Cuts at:      0     122     669 
   Size:        122     547
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     291     669 
   Size:         48     243     378

  Fragments arranged by size: 

                 378     243      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     669 
   Size:        398     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     316     398     472     669 
   Size:        316      82      74     197

  Fragments arranged by size: 

                 316     197      82      74
 
BsrDI GCAATG_nn'

Cuts at:      0     116     669 
   Size:        116     553
 
BstYI r'GATC_y

Cuts at:      0     494     669 
   Size:        494     175
 
BtgI C'CryG_G

Cuts at:      0     362     669 
   Size:        362     307
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     141     669 
   Size:        141     528
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      98     133     669 
   Size:         98      35     536

  Fragments arranged by size: 

                 536      98      35
 
EaeI y'GGCC_r

Cuts at:      0     140     669 
   Size:        140     529
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     669 
   Size:         91     578
 
EcoICRI GAG'CTC

Cuts at:      0     396     669 
   Size:        396     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     266     303     669 
   Size:        170      96      37     366

  Fragments arranged by size: 

                 366     170      96      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     669 
   Size:         53     616
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     365     397     669 
   Size:        166      32     167      32     272

  Fragments arranged by size: 

                 272     167     166      32      32
 
HincII GTy'rAC

Cuts at:      0     284     669 
   Size:        284     385
 
MscI TGG'CCA

Cuts at:      0     142     669 
   Size:        142     527
 
MspA1I CmG'CkG

Cuts at:      0      21      36     547     669 
   Size:         21      15     511     122

  Fragments arranged by size: 

                 511     122      21      15
 
NcoI C'CATG_G

Cuts at:      0     362     669 
   Size:        362     307
 
NsiI A_TGCA'T

Cuts at:      0     183     669 
   Size:        183     486
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      56     669 
   Size:         56     613
 
PmlI CAC'GTG

Cuts at:      0     117     669 
   Size:        117     552
 
PpuMI rG'GwC_Cy

Cuts at:      0      53     669 
   Size:         53     616
 
PshAI GACnn'nnGTC

Cuts at:      0     247     669 
   Size:        247     422
 
PvuII CAG'CTG

Cuts at:      0      36     547     669 
   Size:         36     511     122

  Fragments arranged by size: 

                 511     122      36
 
SacI G_AGCT'C

Cuts at:      0     398     669 
   Size:        398     271
 
StyI C'CwwG_G

Cuts at:      0       9     348     362     669 
   Size:          9     339      14     307

  Fragments arranged by size: 

                 339     307      14       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     317     349     669 
   Size:        317      32     320

  Fragments arranged by size: 

                 320     317      32
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    BamHI     BanI    BanII     BbsI     BcgI
    BfrBI     BlpI Bme1580I     BpmI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BstYI     BtgI    BtgZI    CspCI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII     MscI
   MspA1I     NcoI     NsiI    PflMI     PmlI    PpuMI    PshAI    PvuII
     SacI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI    BciVI
     BclI     BglI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI
     ClaI     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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