Restriction map for
pF1KB8499
(Linear) (Six-base) MAPSORT of: KIBB8499.seq Check: 5634 from: 1 to: 2796
KIBB8499.orf
With 182 enzymes: SgfI *
September 25, 2012 15:38 ..
AccI GT'mk_AC
Cuts at: 0 792 2604 2796
Size: 792 1812 192
Fragments arranged by size:
1812 792 192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1139 2029 2796
Size: 707 432 890 767
Fragments arranged by size:
890 767 707 432
AflII C'TTAA_G
Cuts at: 0 555 2796
Size: 555 2241
AleI CACnn'nnGTG
Cuts at: 0 58 2796
Size: 58 2738
ApoI r'AATT_y
Cuts at: 0 255 924 1029 1271 1475 1515 1547 1980
Size: 255 669 105 242 204 40 32 433
Cuts at: 1980 2124 2555 2796
Size: 144 431 241
Fragments arranged by size:
669 433 431 255 242 241 204 144
105 40 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 410 443 1142 1175 1881 1914 2796
Size: 410 33 699 33 706 33 882
Fragments arranged by size:
882 706 699 410 33 33 33
BanI G'GyrC_C
Cuts at: 0 106 2796
Size: 106 2690
BbsI GAAGACnn'nnnn_
Cuts at: 0 1834 2796
Size: 1834 962
BbvCI CC'TCA_GC
Cuts at: 0 311 2796
Size: 311 2485
BciVI GTATCCnnnnn_n'
Cuts at: 0 550 884 2796
Size: 550 334 1912
Fragments arranged by size:
1912 550 334
BfrBI ATG'CAT
Cuts at: 0 1182 1303 2203 2796
Size: 1182 121 900 593
Fragments arranged by size:
1182 900 593 121
BglII A'GATC_T
Cuts at: 0 246 519 2796
Size: 246 273 2277
Fragments arranged by size:
2277 273 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 351 1389 2796
Size: 351 1038 1407
Fragments arranged by size:
1407 1038 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 2796
Size: 151 2645
Bpu10I CC'TnA_GC
Cuts at: 0 311 2796
Size: 311 2485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2305 2796
Size: 2305 491
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2627 2657 2796
Size: 2627 30 139
Fragments arranged by size:
2627 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 2796
Size: 514 2282
BsiHKAI G_wGCw'C
Cuts at: 0 1737 2796
Size: 1737 1059
BsmI GAATG_Cn'
Cuts at: 0 1305 2796
Size: 1305 1491
Bsp1286I G_dGCh'C
Cuts at: 0 1737 2796
Size: 1737 1059
BspHI T'CATG_A
Cuts at: 0 240 527 2796
Size: 240 287 2269
Fragments arranged by size:
2269 287 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1444 2796
Size: 1444 1352
BsrDI GCAATG_nn'
Cuts at: 0 215 2796
Size: 215 2581
BsrGI T'GTAC_A
Cuts at: 0 570 2796
Size: 570 2226
BstYI r'GATC_y
Cuts at: 0 246 512 519 2093 2353 2796
Size: 246 266 7 1574 260 443
Fragments arranged by size:
1574 443 266 260 246 7
BtsI GCAGTG_nn'
Cuts at: 0 381 2648 2796
Size: 381 2267 148
Fragments arranged by size:
2267 381 148
ClaI AT'CG_AT
Cuts at: 0 896 2796
Size: 896 1900
DraI TTT'AAA
Cuts at: 0 1220 2274 2549 2796
Size: 1220 1054 275 247
Fragments arranged by size:
1220 1054 275 247
EaeI y'GGCC_r
Cuts at: 0 378 2796
Size: 378 2418
EarI CTCTTCn'nnn_
Cuts at: 0 1473 1835 1856 1900 2150 2796
Size: 1473 362 21 44 250 646
Fragments arranged by size:
1473 646 362 250 44 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 707 1139 2029 2796
Size: 151 556 432 890 767
Fragments arranged by size:
890 767 556 432 151
EcoRV GAT'ATC
Cuts at: 0 813 2796
Size: 813 1983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1474 2575 2796
Size: 1474 1101 221
Fragments arranged by size:
1474 1101 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 504 536 1651 1683 2169 2201 2796
Size: 504 32 1115 32 486 32 595
Fragments arranged by size:
1115 595 504 486 32 32 32
HincII GTy'rAC
Cuts at: 0 793 2148 2333 2796
Size: 793 1355 185 463
Fragments arranged by size:
1355 793 463 185
HpaI GTT'AAC
Cuts at: 0 2333 2796
Size: 2333 463
MfeI C'AATT_G
Cuts at: 0 7 2796
Size: 7 2789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 852 2796
Size: 852 1944
MscI TGG'CCA
Cuts at: 0 380 2796
Size: 380 2416
MslI CAynn'nnrTG
Cuts at: 0 58 1187 1318 1424 2796
Size: 58 1129 131 106 1372
Fragments arranged by size:
1372 1129 131 106 58
MspA1I CmG'CkG
Cuts at: 0 910 1066 2796
Size: 910 156 1730
Fragments arranged by size:
1730 910 156
NsiI A_TGCA'T
Cuts at: 0 1184 1305 2205 2796
Size: 1184 121 900 591
Fragments arranged by size:
1184 900 591 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 1976 2533 2796
Size: 1976 557 263
Fragments arranged by size:
1976 557 263
PsiI TTA'TAA
Cuts at: 0 1375 2796
Size: 1375 1421
PstI C_TGCA'G
Cuts at: 0 1485 2307 2796
Size: 1485 822 489
Fragments arranged by size:
1485 822 489
PvuII CAG'CTG
Cuts at: 0 910 1066 2796
Size: 910 156 1730
Fragments arranged by size:
1730 910 156
SalI G'TCGA_C
Cuts at: 0 791 2796
Size: 791 2005
ScaI AGT'ACT
Cuts at: 0 217 2796
Size: 217 2579
SfcI C'TryA_G
Cuts at: 0 905 1481 2303 2703 2796
Size: 905 576 822 400 93
Fragments arranged by size:
905 822 576 400 93
SmlI C'TyrA_G
Cuts at: 0 555 2284 2796
Size: 555 1729 512
Fragments arranged by size:
1729 555 512
SpeI A'CTAG_T
Cuts at: 0 748 1786 2796
Size: 748 1038 1010
Fragments arranged by size:
1038 1010 748
SspI AAT'ATT
Cuts at: 0 1533 2079 2796
Size: 1533 546 717
Fragments arranged by size:
1533 717 546
StyI C'CwwG_G
Cuts at: 0 95 287 2796
Size: 95 192 2509
Fragments arranged by size:
2509 192 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 370 1408 2796
Size: 370 1038 1388
Fragments arranged by size:
1388 1038 370
TatI w'GTAC_w
Cuts at: 0 215 570 798 1024 2669 2796
Size: 215 355 228 226 1645 127
Fragments arranged by size:
1645 355 228 226 215 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2627 2659 2796
Size: 2627 32 137
Fragments arranged by size:
2627 137 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1234 1402 2404 2796
Size: 1234 168 1002 392
Fragments arranged by size:
1234 1002 392 168
XmnI GAAnn'nnTTC
Cuts at: 0 671 2042 2449 2796
Size: 671 1371 407 347
Fragments arranged by size:
1371 671 407 347
Enzymes that do cut:
AccI AcuI AflII AleI ApoI BaeI BanI BbsI
BbvCI BciVI BfrBI BglII BmrI BpmI Bpu10I BpuEI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstYI BtsI ClaI DraI EaeI EarI Eco57MI
EcoRV FalI Hin4I HincII HpaI MfeI MmeI MscI
MslI MspA1I NsiI PflMI PsiI PstI PvuII SalI
ScaI SfcI SmlI SpeI SspI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanII BbeI BcgI BclI BglI
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI
HaeII HindIII KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI