Restriction map for pF1KB7818
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvrII       BamHI       BbeI        
BbvCI       BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiEI       BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FseI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII         214  GACGTC               GACGT^C
AccI         1117  GTCGAC               GT^[AC][GT]AC
AccIII        250  TCCGGA               T^CCGGA
AcsI         1462  GAATTC               [GA]^AATT[CT]
ApoI         1462  GAATTC               [GA]^AATT[CT]
AviII         830  TGCGCA               TGC^GCA
BalI          470  TGGCCA               TGG^CCA
BclI         1091  TGATCA               T^GATCA
BfaI          563  CTAG                 C^TAG
BpmI          107  CTCCAG               CTGGAG(16/14)
BsaWI         250  TCCGGA               [AT]^CCGG[AT]
BseAI         250  TCCGGA               T^CCGGA
BsmBI         719  CGTCTC               CGTCTC(1/5)
BspEI         250  TCCGGA               T^CCGGA
BsrFI         619  GCCGGC               [GA]^CCGG[CT]
BstAPI       1410  GCACAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        619  GCCGGC               [GA]^CCGG[CT]
DraI         1011  TTTAAA               TTT^AAA
EcoRI        1462  GAATTC               G^AATTC
FbaI         1091  TGATCA               T^GATCA
FspI          830  TGCGCA               TGC^GCA
HincII       1117  GTCGAC               GT[CT]^[GA]AC
HindII       1117  GTCGAC               GT[CT]^[GA]AC
MaeI          563  CTAG                 C^TAG
MluI          716  ACGCGT               A^CGCGT
MluNI         470  TGGCCA               TGG^CCA
MroI          250  TCCGGA               T^CCGGA
MscI          470  TGGCCA               TGG^CCA
MspA1I        724  CAGCTG               C[AC]G^C[GT]G
NaeI          619  GCCGGC               GCC^GGC
NgoMIV        619  GCCGGC               G^CCGGC
PpuMI          25  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          703  CTGCAG               CTGCA^G
PvuII         724  CAGCTG               CAG^CTG
SalI         1117  GTCGAC               G^TCGAC
SapI         1047  GAAGAGC              GCTCTTC(1/4)
ScaI          947  AGTACT               AGT^ACT


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          214  GACGTC               G[GA]^CG[CT]C
              384  GACGCC              
AflIII        716  ACGCGT               A^C[GA][CT]GT
              890  ACGTGT              
AlwI         1269  GATCC                GGATC(4/5)
              439  GGATC               
ApaI          586  GGGCCC               GGGCC^C
             1109  GGGCCC              
Asp700I       277  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              991  GAAGTGGTTC          
BbrPI         631  CACGTG               CAC^GTG
             1612  CACGTG              
BbsI          277  GAAGAC               GAAGAC(2/6)
             1735  GAAGAC              
BcgI         1494  CGACATTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1419  GCAATGCGTTCG        
BpuAI         277  GAAGAC               GAAGAC(2/6)
             1735  GAAGAC              
BsaHI         214  GACGTC               G[GA]^CG[CT]C
              384  GACGCC              
BsgI          592  CTGCAC               GTGCAG(16/14)
              409  GTGCAG              
BstF5I       1248  CATCC                GGATG(2/0)
              980  GGATG               
BstYI         439  GGATCT               [GA]^GATC[CT]
             1268  AGATCC              
DraIII        628  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              631  CACGTGGTG           
DsaI          686  CCATGG               C^C[GA][CT]GG
             1238  CCATGG              
EaeI          470  TGGCCA               [CT]^GGCC[GA]
              711  CGGCCA              
EcoT14I       686  CCATGG               C^C[AT][AT]GG
             1238  CCATGG              
FokI         1248  CATCC                GGATG(9/13)
              980  GGATG               
Hin1I         214  GACGTC               G[GA]^CG[CT]C
              384  GACGCC              
MflI          439  GGATCT               [GA]^GATC[CT]
             1268  AGATCC              
NcoI          686  CCATGG               C^CATGG
             1238  CCATGG              
PflMI         627  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              630  CCACGTGGTGG         
PleI         1120  GACTC                GAGTC(4/5)
             1086  GAGTC               
PmaCI         631  CACGTG               CAC^GTG
             1612  CACGTG              
PmlI          631  CACGTG               CAC^GTG
             1612  CACGTG              
PshAI         254  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              922  GACAAGCGTC          
SmlI           35  CTCAAG               C^T[CT][GA]AG
             1552  CTCAAG              
StyI          686  CCATGG               C^C[AT][AT]GG
             1238  CCATGG              
Tsp45I       1534  GTCAC                ^GT[GC]AC
             1615  GTGAC               
Van91I        627  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              630  CCACGTGGTGG         
XhoII         439  GGATCT               [GA]^GATC[CT]
             1268  AGATCC              
XmnI          277  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              991  GAAGTGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XmaCI       XmaI        
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