Restriction map for pF1KB7818
 (Linear) (Six-base) MAPSORT of: KIBB7818.seq  Check: 1585  from: 1  to: 1794



KIBB7818.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     323     729    1794 
   Size:        323     406    1065

  Fragments arranged by size: 

                1065     406     323
 
AatII G_ACGT'C

Cuts at:      0     218    1794 
   Size:        218    1576
 
AccI GT'mk_AC

Cuts at:      0    1118    1794 
   Size:       1118     676
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162    1794 
   Size:        162    1632
 
AflIII A'CryG_T

Cuts at:      0     716     890    1794 
   Size:        716     174     904

  Fragments arranged by size: 

                 904     716     174
 
AleI CACnn'nnGTG

Cuts at:      0     556     739    1794 
   Size:        556     183    1055

  Fragments arranged by size: 

                1055     556     183
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     896    1794 
   Size:        896     898
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1325    1357    1794 
   Size:       1325      32     437

  Fragments arranged by size: 

                1325     437      32
 
ApaI G_GGCC'C

Cuts at:      0     590    1113    1794 
   Size:        590     523     681

  Fragments arranged by size: 

                 681     590     523
 
ApoI r'AATT_y

Cuts at:      0    1462    1794 
   Size:       1462     332
 
AvaI C'yCGr_G

Cuts at:      0     396     435     696    1794 
   Size:        396      39     261    1098

  Fragments arranged by size: 

                1098     396     261      39
 
BanI G'GyrC_C

Cuts at:      0     550     741    1743    1794 
   Size:        550     191    1002      51

  Fragments arranged by size: 

                1002     550     191      51
 
BanII G_rGCy'C

Cuts at:      0     590     617     766    1054    1064    1113    1553    1794 
   Size:        590      27     149     288      10      49     440     241

  Fragments arranged by size: 

                 590     440     288     241     149      49      27      10
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     284    1742    1794 
   Size:        284    1458      52

  Fragments arranged by size: 

                1458     284      52
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1408    1442    1483    1517    1794 
   Size:       1408      34      41      34     277

  Fragments arranged by size: 

                1408     277      41      34      34
 
BclI T'GATC_A

Cuts at:      0    1091    1794 
   Size:       1091     703
 
Bme1580I G_kGCm'C

Cuts at:      0     590     662     746    1101    1113    1794 
   Size:        590      72      84     355      12     681

  Fragments arranged by size: 

                 681     590     355      84      72      12
 
BmgBI CAC'GTC

Cuts at:      0     597    1794 
   Size:        597    1197
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     318     910    1363    1794 
   Size:        318     592     453     431

  Fragments arranged by size: 

                 592     453     431     318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1794 
   Size:         92    1702
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    1537    1794 
   Size:         20    1517     257

  Fragments arranged by size: 

                1517     257      20
 
BsaAI yAC'GTr

Cuts at:      0     468     633     891    1614    1794 
   Size:        468     165     258     723     180

  Fragments arranged by size: 

                 723     468     258     180     165
 
BsaHI Gr'CG_yC

Cuts at:      0     215     385    1794 
   Size:        215     170    1409

  Fragments arranged by size: 

                1409     215     170
 
BsaWI w'CCGG_w

Cuts at:      0     250    1794 
   Size:        250    1544
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385    1014    1044    1794 
   Size:        355      30     629      30     750

  Fragments arranged by size: 

                 750     629     355      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     363    1458    1794 
   Size:        346      17    1095     336

  Fragments arranged by size: 

                1095     346     336      17
 
BseYI C'CCAG_C

Cuts at:      0     172    1199    1256    1794 
   Size:        172    1027      57     538

  Fragments arranged by size: 

                1027     538     172      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     577    1794 
   Size:        430     147    1217

  Fragments arranged by size: 

                1217     430     147
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     725    1794 
   Size:        725    1069
 
Bsp1286I G_dGCh'C

Cuts at:      0     590     617     662     746     766    1054    1064    1101 
   Size:        590      27      45      84      20     288      10      37

Cuts at:   1101    1113    1553    1794 
   Size:         12     440     241

  Fragments arranged by size: 

                 590     440     288     241      84      45      37      27
                  20      12      10
 
BspEI T'CCGG_A

Cuts at:      0     250    1794 
   Size:        250    1544
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     323     729    1295    1316    1794 
   Size:        323     406     566      21     478

  Fragments arranged by size: 

                 566     478     406     323      21
 
BsrDI GCAATG_nn'

Cuts at:      0    1401    1426    1514    1794 
   Size:       1401      25      88     280

  Fragments arranged by size: 

                1401     280      88      25
 
BsrFI r'CCGG_y

Cuts at:      0     619    1794 
   Size:        619    1175
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1416    1794 
   Size:       1416     378
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1371    1569    1665    1794 
   Size:       1371     198      96     129

  Fragments arranged by size: 

                1371     198     129      96
 
BstYI r'GATC_y

Cuts at:      0     439    1268    1794 
   Size:        439     829     526

  Fragments arranged by size: 

                 829     526     439
 
BtgI C'CryG_G

Cuts at:      0     686    1238    1794 
   Size:        686     552     556

  Fragments arranged by size: 

                 686     556     552
 
BtsI GCAGTG_nn'

Cuts at:      0     152    1794 
   Size:        152    1642
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     212     247    1794 
   Size:        212      35    1547

  Fragments arranged by size: 

                1547     212      35
 
DraI TTT'AAA

Cuts at:      0    1013    1794 
   Size:       1013     781
 
DraIII CAC_nnn'GTG

Cuts at:      0     633     636    1794 
   Size:        633       3    1158

  Fragments arranged by size: 

                1158     633       3
 
EaeI y'GGCC_r

Cuts at:      0     470     711    1794 
   Size:        470     241    1083

  Fragments arranged by size: 

                1083     470     241
 
EarI CTCTTCn'nnn_

Cuts at:      0      81     529    1042    1794 
   Size:         81     448     513     752

  Fragments arranged by size: 

                 752     513     448      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     162    1794 
   Size:         92      70    1632

  Fragments arranged by size: 

                1632      92      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0      26     587    1109    1794 
   Size:         26     561     522     685

  Fragments arranged by size: 

                 685     561     522      26
 
EcoRI G'AATT_C

Cuts at:      0    1462    1794 
   Size:       1462     332
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1327    1794 
   Size:       1327     467
 
FspI TGC'GCA

Cuts at:      0     832    1794 
   Size:        832     962
 
FspAI rTGC'GCAy

Cuts at:      0     832    1794 
   Size:        832     962
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     424     456    1535    1567    1794 
   Size:        424      32    1079      32     227

  Fragments arranged by size: 

                1079     424     227      32      32
 
HincII GTy'rAC

Cuts at:      0    1119    1794 
   Size:       1119     675
 
MluI A'CGCG_T

Cuts at:      0     716    1794 
   Size:        716    1078
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1148    1388    1496    1794 
   Size:        797     351     240     108     298

  Fragments arranged by size: 

                 797     351     298     240     108
 
MscI TGG'CCA

Cuts at:      0     472    1794 
   Size:        472    1322
 
MslI CAynn'nnrTG

Cuts at:      0     286     556     739    1794 
   Size:        286     270     183    1055

  Fragments arranged by size: 

                1055     286     270     183
 
MspA1I CmG'CkG

Cuts at:      0     726    1794 
   Size:        726    1068
 
NaeI GCC'GGC

Cuts at:      0     621    1794 
   Size:        621    1173
 
NcoI C'CATG_G

Cuts at:      0     686    1238    1794 
   Size:        686     552     556

  Fragments arranged by size: 

                 686     556     552
 
NgoMIV G'CCGG_C

Cuts at:      0     619    1794 
   Size:        619    1175
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     633     636    1794 
   Size:        633       3    1158

  Fragments arranged by size: 

                1158     633       3
 
PfoI T'CCnGG_A

Cuts at:      0    1775    1794 
   Size:       1775      19
 
PmlI CAC'GTG

Cuts at:      0     633    1614    1794 
   Size:        633     981     180

  Fragments arranged by size: 

                 981     633     180
 
PpuMI rG'GwC_Cy

Cuts at:      0      26    1794 
   Size:         26    1768
 
PshAI GACnn'nnGTC

Cuts at:      0     258     926    1794 
   Size:        258     668     868

  Fragments arranged by size: 

                 868     668     258
 
PspOMI G'GGCC_C

Cuts at:      0     586    1109    1794 
   Size:        586     523     685

  Fragments arranged by size: 

                 685     586     523
 
PstI C_TGCA'G

Cuts at:      0     707    1794 
   Size:        707    1087
 
PvuII CAG'CTG

Cuts at:      0     726    1794 
   Size:        726    1068
 
SalI G'TCGA_C

Cuts at:      0    1117    1794 
   Size:       1117     677
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1042    1794 
   Size:       1042     752
 
ScaI AGT'ACT

Cuts at:      0     949    1794 
   Size:        949     845
 
SfcI C'TryA_G

Cuts at:      0      63     198     703    1708    1794 
   Size:         63     135     505    1005      86

  Fragments arranged by size: 

                1005     505     135      86      63
 
SmaI CCC'GGG

Cuts at:      0     398     437     698    1794 
   Size:        398      39     261    1096

  Fragments arranged by size: 

                1096     398     261      39
 
SmlI C'TyrA_G

Cuts at:      0      35    1552    1794 
   Size:         35    1517     242

  Fragments arranged by size: 

                1517     242      35
 
StyI C'CwwG_G

Cuts at:      0     686    1238    1794 
   Size:        686     552     556

  Fragments arranged by size: 

                 686     556     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1213    1794 
   Size:       1213     581
 
TatI w'GTAC_w

Cuts at:      0     947    1794 
   Size:        947     847
 
XmaI C'CCGG_G

Cuts at:      0     396     435     696    1794 
   Size:        396      39     261    1098

  Fragments arranged by size: 

                1098     396     261      39
 
XmnI GAAnn'nnTTC

Cuts at:      0     281     995    1794 
   Size:        281     714     799

  Fragments arranged by size: 

                 799     714     281
 
ZraI GAC'GTC

Cuts at:      0     216    1794 
   Size:        216    1578
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI     AloI
     ApaI     ApoI     AvaI     BanI    BanII     BbsI     BcgI     BclI
 Bme1580I    BmgBI     BmrI     BpmI    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI     BtsI    CspCI
     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    FspAI    Hin4I   HincII     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV    PflMI     PfoI     PmlI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SalI     SapI     ScaI     SfcI
     SmaI     SmlI     StyI    TaqII     TatI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI    AlwNI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI    BfrBI     BglI    BglII     BlpI     BmtI     BplI   Bpu10I
     BsaI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI
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