Restriction map for pF1KE2394
 (Linear) (Six-base) MAPSORT of: KIEE2394.seq  Check: 4074  from: 1  to: 2463



KIEE2394.orf

 With 182 enzymes: SgfI * 

                        January 26, 2012 13:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     408    2463 
   Size:        408    2055
 
AatII G_ACGT'C

Cuts at:      0     761    1492    2463 
   Size:        761     731     971

  Fragments arranged by size: 

                 971     761     731
 
AccI GT'mk_AC

Cuts at:      0    1250    2463 
   Size:       1250    1213
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1904    2306    2463 
   Size:        710    1194     402     157

  Fragments arranged by size: 

                1194     710     402     157
 
AflII C'TTAA_G

Cuts at:      0    2136    2463 
   Size:       2136     327
 
AflIII A'CryG_T

Cuts at:      0    1929    2463 
   Size:       1929     534
 
AleI CACnn'nnGTG

Cuts at:      0    1907    2463 
   Size:       1907     556
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45      84    1294    2463 
   Size:         45      39    1210    1169

  Fragments arranged by size: 

                1210    1169      45      39
 
ApaI G_GGCC'C

Cuts at:      0     858    2463 
   Size:        858    1605
 
ApoI r'AATT_y

Cuts at:      0    1381    1560    2463 
   Size:       1381     179     903

  Fragments arranged by size: 

                1381     903     179
 
AvaI C'yCGr_G

Cuts at:      0     647    2463 
   Size:        647    1816
 
BanI G'GyrC_C

Cuts at:      0      28    2463 
   Size:         28    2435
 
BanII G_rGCy'C

Cuts at:      0     377     858     944    2369    2463 
   Size:        377     481      86    1425      94

  Fragments arranged by size: 

                1425     481     377      94      86
 
BbeI G_GCGC'C

Cuts at:      0      32    2463 
   Size:         32    2431
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1826    2108    2463 
   Size:       1826     282     355

  Fragments arranged by size: 

                1826     355     282
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     573     667    2463 
   Size:        573      94    1796

  Fragments arranged by size: 

                1796     573      94
 
BclI T'GATC_A

Cuts at:      0     491    2349    2463 
   Size:        491    1858     114

  Fragments arranged by size: 

                1858     491     114
 
BlpI GC'TnA_GC

Cuts at:      0     638     769    2463 
   Size:        638     131    1694

  Fragments arranged by size: 

                1694     638     131
 
Bme1580I G_kGCm'C

Cuts at:      0     858    2463 
   Size:        858    1605
 
BmgBI CAC'GTC

Cuts at:      0    1398    2463 
   Size:       1398    1065
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     430    2463 
   Size:        430    2033
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     396    2298    2463 
   Size:        396    1902     165

  Fragments arranged by size: 

                1902     396     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1358    1841    2463 
   Size:         67    1291     483     622

  Fragments arranged by size: 

                1291     622     483      67
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    1201    1882    2463 
   Size:        692     509     681     581

  Fragments arranged by size: 

                 692     681     581     509
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     622    2463 
   Size:        622    1841
 
BsaAI yAC'GTr

Cuts at:      0     787    1224    2463 
   Size:        787     437    1239

  Fragments arranged by size: 

                1239     787     437
 
BsaBI GATnn'nnATC

Cuts at:      0    1031    1539    1991    2463 
   Size:       1031     508     452     472

  Fragments arranged by size: 

                1031     508     472     452
 
BsaHI Gr'CG_yC

Cuts at:      0      29     758    1489    2463 
   Size:         29     729     731     974

  Fragments arranged by size: 

                 974     731     729      29
 
BsaWI w'CCGG_w

Cuts at:      0    1946    2463 
   Size:       1946     517
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     994    1024    2463 
   Size:        994      30    1439

  Fragments arranged by size: 

                1439     994      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     256     579    1243    2247    2463 
   Size:        118     138     323     664    1004     216

  Fragments arranged by size: 

                1004     664     323     216     138     118
 
BseYI C'CCAG_C

Cuts at:      0    2142    2463 
   Size:       2142     321
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    2463 
   Size:        809    1654
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     786     944    2369    2463 
   Size:        377     409     158    1425      94

  Fragments arranged by size: 

                1425     409     377     158      94
 
BsmI GAATG_Cn'

Cuts at:      0     337     839    1665    2463 
   Size:        337     502     826     798

  Fragments arranged by size: 

                 826     798     502     337
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     765    1882    2463 
   Size:        765    1117     581

  Fragments arranged by size: 

                1117     765     581
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     786     858     944    2369    2463 
   Size:        377     409      72      86    1425      94

  Fragments arranged by size: 

                1425     409     377      94      86      72
 
BspHI T'CATG_A

Cuts at:      0    1317    1419    2463 
   Size:       1317     102    1044

  Fragments arranged by size: 

                1317    1044     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     356     408    2463 
   Size:        356      52    2055

  Fragments arranged by size: 

                2055     356      52
 
BsrBI CCG'CTC

Cuts at:      0     458    2463 
   Size:        458    2005
 
BsrDI GCAATG_nn'

Cuts at:      0    1086    1640    2463 
   Size:       1086     554     823

  Fragments arranged by size: 

                1086     823     554
 
BsrFI r'CCGG_y

Cuts at:      0      31    2463 
   Size:         31    2432
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     423    2463 
   Size:        423    2040
 
BstEII G'GTnAC_C

Cuts at:      0    1186    2463 
   Size:       1186    1277
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1813    2463 
   Size:       1813     650
 
BstYI r'GATC_y

Cuts at:      0     308    2012    2421    2463 
   Size:        308    1704     409      42

  Fragments arranged by size: 

                1704     409     308      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     134    2463 
   Size:        134    2329
 
BtsI GCAGTG_nn'

Cuts at:      0    1902    2463 
   Size:       1902     561
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1030    1065    2463 
   Size:       1030      35    1398

  Fragments arranged by size: 

                1398    1030      35
 
EaeI y'GGCC_r

Cuts at:      0      65    2463 
   Size:         65    2398
 
EarI CTCTTCn'nnn_

Cuts at:      0    1160    1600    2003    2204    2463 
   Size:       1160     440     403     201     259

  Fragments arranged by size: 

                1160     440     403     259     201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1792    2463 
   Size:       1792     671
 
EcoICRI GAG'CTC

Cuts at:      0     375     942    2367    2463 
   Size:        375     567    1425      96

  Fragments arranged by size: 

                1425     567     375      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     710    1358    1841    1904    2306    2463 
   Size:         67     643     648     483      63     402     157

  Fragments arranged by size: 

                 648     643     483     402     157      67      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2136    2194    2463 
   Size:       2136      58     269

  Fragments arranged by size: 

                2136     269      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     855     870    1148    2463 
   Size:        855      15     278    1315

  Fragments arranged by size: 

                1315     855     278      15
 
EcoRV GAT'ATC

Cuts at:      0    1713    1723    2463 
   Size:       1713      10     740

  Fragments arranged by size: 

                1713     740      10
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     658     744    2311    2463 
   Size:        658      86    1567     152

  Fragments arranged by size: 

                1567     658     152      86
 
HaeII r_GCGC'y

Cuts at:      0      32    2463 
   Size:         32    2431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     641     673    1297    1329    1577    1609    1712    1744 
   Size:        641      32     624      32     248      32     103      32

Cuts at:   1744    2203    2235    2463 
   Size:        459      32     228

  Fragments arranged by size: 

                 641     624     459     248     228     103      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2247    2439    2463 
   Size:       2247     192      24

  Fragments arranged by size: 

                2247     192      24
 
HindIII A'AGCT_T

Cuts at:      0     766    1940    2463 
   Size:        766    1174     523

  Fragments arranged by size: 

                1174     766     523
 
HpaI GTT'AAC

Cuts at:      0    2247    2439    2463 
   Size:       2247     192      24

  Fragments arranged by size: 

                2247     192      24
 
KasI G'GCGC_C

Cuts at:      0      28    2463 
   Size:         28    2435
 
MfeI C'AATT_G

Cuts at:      0    1685    2463 
   Size:       1685     778
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1944    2463 
   Size:       1944     519
 
MslI CAynn'nnrTG

Cuts at:      0    1079    1907    1972    2463 
   Size:       1079     828      65     491

  Fragments arranged by size: 

                1079     828     491      65
 
MspA1I CmG'CkG

Cuts at:      0      19     964    1294    2463 
   Size:         19     945     330    1169

  Fragments arranged by size: 

                1169     945     330      19
 
NaeI GCC'GGC

Cuts at:      0      33    2463 
   Size:         33    2430
 
NarI GG'CG_CC

Cuts at:      0      29    2463 
   Size:         29    2434
 
NgoMIV G'CCGG_C

Cuts at:      0      31    2463 
   Size:         31    2432
 
NspI r_CATG'y

Cuts at:      0    1933    2463 
   Size:       1933     530
 
PciI A'CATG_T

Cuts at:      0    1929    2463 
   Size:       1929     534
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45     928    1519    2463 
   Size:         45     883     591     944

  Fragments arranged by size: 

                 944     883     591      45
 
PfoI T'CCnGG_A

Cuts at:      0      89    2463 
   Size:         89    2374
 
PmlI CAC'GTG

Cuts at:      0     787    1224    2463 
   Size:        787     437    1239

  Fragments arranged by size: 

                1239     787     437
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1800    1832    2463 
   Size:       1800      32     631

  Fragments arranged by size: 

                1800     631      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     870    1148    2463 
   Size:        870     278    1315

  Fragments arranged by size: 

                1315     870     278
 
PsiI TTA'TAA

Cuts at:      0    2299    2463 
   Size:       2299     164
 
PspOMI G'GGCC_C

Cuts at:      0     854    2463 
   Size:        854    1609
 
PspXI vC'TCGA_Gb

Cuts at:      0     647    2463 
   Size:        647    1816
 
PvuII CAG'CTG

Cuts at:      0     964    1294    2463 
   Size:        964     330    1169

  Fragments arranged by size: 

                1169     964     330
 
SacI G_AGCT'C

Cuts at:      0     377     944    2369    2463 
   Size:        377     567    1425      94

  Fragments arranged by size: 

                1425     567     377      94
 
ScaI AGT'ACT

Cuts at:      0    2158    2463 
   Size:       2158     305
 
SfcI C'TryA_G

Cuts at:      0     249    2463 
   Size:        249    2214
 
SfoI GGC'GCC

Cuts at:      0      30    2463 
   Size:         30    2433
 
SmlI C'TyrA_G

Cuts at:      0     647     707    1216    1861    2136    2463 
   Size:        647      60     509     645     275     327

  Fragments arranged by size: 

                 647     645     509     327     275      60
 
StyI C'CwwG_G

Cuts at:      0     320     858     922    2463 
   Size:        320     538      64    1541

  Fragments arranged by size: 

                1541     538     320      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     449     458    2386    2463 
   Size:        449       9    1928      77

  Fragments arranged by size: 

                1928     449      77       9
 
TatI w'GTAC_w

Cuts at:      0    1195    2156    2463 
   Size:       1195     961     307

  Fragments arranged by size: 

                1195     961     307
 
Tth111I GACn'n_nGTC

Cuts at:      0    1830    2463 
   Size:       1830     633
 
XbaI T'CTAG_A

Cuts at:      0    1995    2463 
   Size:       1995     468
 
XhoI C'TCGA_G

Cuts at:      0     647    2463 
   Size:        647    1816
 
XmnI GAAnn'nnTTC

Cuts at:      0      85     158     286     839    2463 
   Size:         85      73     128     553    1624

  Fragments arranged by size: 

                1624     553     128      85      73
 
ZraI GAC'GTC

Cuts at:      0     759    1490    2463 
   Size:        759     731     973

  Fragments arranged by size: 

                 973     759     731
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII   AflIII     AleI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BciVI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI    BtgZI     BtsI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI    PvuII     SacI     ScaI     SfcI     SfoI     SmlI     StyI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BfrBI     BglI    BglII     BmtI   Bpu10I    BsiEI    BsiWI    BspEI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI     ClaI
     DraI   DraIII     DrdI     EagI    EcoRI     FseI     FspI    FspAI
     KpnI     MluI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI    PshAI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI     XcmI     XmaI
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