Restriction map for pF1KE2354
 (Linear) (Six-base) MAPSORT of: KIEE2354.seq  Check: 4121  from: 1  to: 9672



KIEE2354.orf

 With 182 enzymes: SgfI * 

                        February 15, 2013 16:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4560    4941    5118    5292    9672 
   Size:       4560     381     177     174    4380

  Fragments arranged by size: 

                4560    4380     381     177     174
 
AatII G_ACGT'C

Cuts at:      0     494    9672 
   Size:        494    9178
 
AccI GT'mk_AC

Cuts at:      0    1907    4527    8088    9672 
   Size:       1907    2620    3561    1584

  Fragments arranged by size: 

                3561    2620    1907    1584
 
Acc65I G'GTAC_C

Cuts at:      0    8358    9672 
   Size:       8358    1314
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1285    1729    2123    2603    3197    4559    4748 
   Size:        781     504     444     394     480     594    1362     189

Cuts at:   4748    4925    5102    5438    5846    6304    6416    6530    6789 
   Size:        177     177     336     408     458     112     114     259

Cuts at:   6789    6851    7460    7748    7889    8369    8399    9270    9547 
   Size:         62     609     288     141     480      30     871     277

Cuts at:   9547    9672 
   Size:        125

  Fragments arranged by size: 

                1362     871     781     609     594     504     480     480
                 458     444     408     394     336     288     277     259
                 189     177     177     141     125     114     112      62
                  30
 
AflII C'TTAA_G

Cuts at:      0     631    1080    9672 
   Size:        631     449    8592

  Fragments arranged by size: 

                8592     631     449
 
AflIII A'CryG_T

Cuts at:      0    1067    4613    9150    9672 
   Size:       1067    3546    4537     522

  Fragments arranged by size: 

                4537    3546    1067     522
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2759    9672 
   Size:       2759    6913
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1633    2554    4741    5920    7558    7621    9672 
   Size:       1633     921    2187    1179    1638      63    2051

  Fragments arranged by size: 

                2187    2051    1638    1633    1179     921      63
 
ApaI G_GGCC'C

Cuts at:      0    2701    6659    9672 
   Size:       2701    3958    3013

  Fragments arranged by size: 

                3958    3013    2701
 
ApoI r'AATT_y

Cuts at:      0     609    1332    2421    2855    2998    3094    3105    3323 
   Size:        609     723    1089     434     143      96      11     218

Cuts at:   3323    3363    3577    3686    4026    4033    4372    5578    5601 
   Size:         40     214     109     340       7     339    1206      23

Cuts at:   5601    5641    5695    6182    6373    6400    6412    7301    8604 
   Size:         40      54     487     191      27      12     889    1303

Cuts at:   8604    8792    9126    9327    9424    9438    9672 
   Size:        188     334     201      97      14     234

  Fragments arranged by size: 

                1303    1206    1089     889     723     609     487     434
                 340     339     334     234     218     214     201     191
                 188     143     109      97      96      54      40      40
                  27      23      14      12      11       7
 
AseI AT'TA_AT

Cuts at:      0     158     997    9672 
   Size:        158     839    8675

  Fragments arranged by size: 

                8675     839     158
 
BamHI G'GATC_C

Cuts at:      0     174    8909    9672 
   Size:        174    8735     763

  Fragments arranged by size: 

                8735     763     174
 
BanI G'GyrC_C

Cuts at:      0    6613    8358    9672 
   Size:       6613    1745    1314

  Fragments arranged by size: 

                6613    1745    1314
 
BanII G_rGCy'C

Cuts at:      0      52     933    1692    2592    2701    4154    4486    5473 
   Size:         52     881     759     900     109    1453     332     987

Cuts at:   5473    6659    8543    9672 
   Size:       1186    1884    1129

  Fragments arranged by size: 

                1884    1453    1186    1129     987     900     881     759
                 332     109      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     193    1291    1949    2081    2267    4616    7754    8240 
   Size:        193    1098     658     132     186    2349    3138     486

Cuts at:   8240    8258    9672 
   Size:         18    1414

  Fragments arranged by size: 

                3138    2349    1414    1098     658     486     193     186
                 132      18
 
BbvCI CC'TCA_GC

Cuts at:      0    6140    9672 
   Size:       6140    3532
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1255    1289    9672 
   Size:       1255      34    8383

  Fragments arranged by size: 

                8383    1255      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1182    6975    9582    9672 
   Size:       1182    5793    2607      90

  Fragments arranged by size: 

                5793    2607    1182      90
 
BclI T'GATC_A

Cuts at:      0    1779    6558    9672 
   Size:       1779    4779    3114

  Fragments arranged by size: 

                4779    3114    1779
 
BfrBI ATG'CAT

Cuts at:      0     873    9672 
   Size:        873    8799
 
BglII A'GATC_T

Cuts at:      0     282    1446    2974    3784    4386    4417    5844    9672 
   Size:        282    1164    1528     810     602      31    1427    3828

  Fragments arranged by size: 

                3828    1528    1427    1164     810     602     282      31
            
 
BlpI GC'TnA_GC

Cuts at:      0    7925    9672 
   Size:       7925    1747
 
Bme1580I G_kGCm'C

Cuts at:      0    1067    2213    2701    3483    6659    9672 
   Size:       1067    1146     488     782    3176    3013

  Fragments arranged by size: 

                3176    3013    1146    1067     782     488
 
BmgBI CAC'GTC

Cuts at:      0      21    9672 
   Size:         21    9651
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2649    2695    4263    5476    6662    7631    8655    9672 
   Size:       2649      46    1568    1213    1186     969    1024    1017

  Fragments arranged by size: 

                2649    1568    1213    1186    1024    1017     969      46
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2299    4500    9672 
   Size:       2299    2201    5172

  Fragments arranged by size: 

                5172    2299    2201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664    2236    5942    7145    8594    8645    8795    9124 
   Size:        664    1572    3706    1203    1449      51     150     329

Cuts at:   9124    9323    9672 
   Size:        199     349

  Fragments arranged by size: 

                3706    1572    1449    1203     664     349     329     199
                 150      51
 
Bpu10I CC'TnA_GC

Cuts at:      0    5771    6140    9672 
   Size:       5771     369    3532

  Fragments arranged by size: 

                5771    3532     369
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1389    6884    9672 
   Size:       1389    5495    2788

  Fragments arranged by size: 

                5495    2788    1389
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3208    8918    9175    9672 
   Size:       3208    5710     257     497

  Fragments arranged by size: 

                5710    3208     497     257
 
BsaBI GATnn'nnATC

Cuts at:      0    2180    2561    9672 
   Size:       2180     381    7111

  Fragments arranged by size: 

                7111    2180     381
 
BsaHI Gr'CG_yC

Cuts at:      0     491    3943    9672 
   Size:        491    3452    5729

  Fragments arranged by size: 

                5729    3452     491
 
BsaWI w'CCGG_w

Cuts at:      0    4704    5977    8906    9672 
   Size:       4704    1273    2929     766

  Fragments arranged by size: 

                4704    2929    1273     766
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1507    1537    2325    2355    2934    2964    4810    4840 
   Size:       1507      30     788      30     579      30    1846      30

Cuts at:   4840    4987    5017    8618    8648    9672 
   Size:        147      30    3601      30    1024

  Fragments arranged by size: 

                3601    1846    1507    1024     788     579     147      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    7520    8026    9386    9672 
   Size:       7520     506    1360     286

  Fragments arranged by size: 

                7520    1360     506     286
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     775    9672 
   Size:         64     711    8897

  Fragments arranged by size: 

                8897     711      64
 
BsiEI CG_ry'CG

Cuts at:      0    6611    9672 
   Size:       6611    3061
 
BsiHKAI G_wGCw'C

Cuts at:      0     795     933    1287    4154    4486    9294    9672 
   Size:        795     138     354    2867     332    4808     378

  Fragments arranged by size: 

                4808    2867     795     378     354     332     138
 
BsmI GAATG_Cn'

Cuts at:      0    1744    2122    4098    6221    6427    7600    9672 
   Size:       1744     378    1976    2123     206    1173    2072

  Fragments arranged by size: 

                2123    2072    1976    1744    1173     378     206
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2345    3696    9672 
   Size:       2345    1351    5976

  Fragments arranged by size: 

                5976    2345    1351
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     795     933    1067    1287    1692    2213    2592 
   Size:         52     743     138     134     220     405     521     379

Cuts at:   2592    2701    3483    4154    4486    5473    6659    8543    9294 
   Size:        109     782     671     332     987    1186    1884     751

Cuts at:   9294    9672 
   Size:        378

  Fragments arranged by size: 

                1884    1186     987     782     751     743     671     521
                 405     379     378     332     220     138     134     109
                  52
 
BspEI T'CCGG_A

Cuts at:      0    4704    9672 
   Size:       4704    4968
 
BspHI T'CATG_A

Cuts at:      0    3459    3984    9672 
   Size:       3459     525    5688

  Fragments arranged by size: 

                5688    3459     525
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1722    4539    4560    4941    5118    5292    9672 
   Size:       1722    2817      21     381     177     174    4380

  Fragments arranged by size: 

                4380    2817    1722     381     177     174      21
 
BsrBI CCG'CTC

Cuts at:      0      27    4790    4967    5144    9672 
   Size:         27    4763     177     177    4528

  Fragments arranged by size: 

                4763    4528     177     177      27
 
BsrDI GCAATG_nn'

Cuts at:      0    1336    2827    3657    4304    9672 
   Size:       1336    1491     830     647    5368

  Fragments arranged by size: 

                5368    1491    1336     830     647
 
BsrFI r'CCGG_y

Cuts at:      0    6610    8668    9672 
   Size:       6610    2058    1004

  Fragments arranged by size: 

                6610    2058    1004
 
BsrGI T'GTAC_A

Cuts at:      0     621    1570    1725    5373    9672 
   Size:        621     949     155    3648    4299

  Fragments arranged by size: 

                4299    3648     949     621     155
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1071    2760    2965    4652    4841    5018    5195    5848 
   Size:       1071    1689     205    1687     189     177     177     653

Cuts at:   5848    9672 
   Size:       3824

  Fragments arranged by size: 

                3824    1689    1687    1071     653     205     189     177
                 177
 
BstBI TT'CG_AA

Cuts at:      0    8030    9672 
   Size:       8030    1642
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2405    5794    9192    9672 
   Size:       2405    3389    3398     480

  Fragments arranged by size: 

                3398    3389    2405     480
 
BstYI r'GATC_y

Cuts at:      0     174     282    1446    2974    3784    4342    4386    4417 
   Size:        174     108    1164    1528     810     558      44      31

Cuts at:   4417    5844    7699    8909    9672 
   Size:       1427    1855    1210     763

  Fragments arranged by size: 

                1855    1528    1427    1210    1164     810     763     558
                 174     108      44      31
 
Bsu36I CC'TnA_GG

Cuts at:      0    1184    3863    9672 
   Size:       1184    2679    5809

  Fragments arranged by size: 

                5809    2679    1184
 
BtgI C'CryG_G

Cuts at:      0    3402    3483    3938    5799    9476    9672 
   Size:       3402      81     455    1861    3677     196

  Fragments arranged by size: 

                3677    3402    1861     455     196      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      18    1223    6038    9672 
   Size:         18    1205    4815    3634

  Fragments arranged by size: 

                4815    3634    1205      18
 
BtsI GCAGTG_nn'

Cuts at:      0     558    4461    4842    5019    5196    7526    9068    9672 
   Size:        558    3903     381     177     177    2330    1542     604

  Fragments arranged by size: 

                3903    2330    1542     604     558     381     177     177
            
 
ClaI AT'CG_AT

Cuts at:      0    4218    9672 
   Size:       4218    5454
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    7502    7537    9672 
   Size:       7502      35    2135

  Fragments arranged by size: 

                7502    2135      35
 
DraI TTT'AAA

Cuts at:      0    1106    3093    3882    3914    4371    5523    5694    6378 
   Size:       1106    1987     789      32     457    1152     171     684

Cuts at:   6378    6813    7269    7833    8802    8937    9072    9672 
   Size:        435     456     564     969     135     135     600

  Fragments arranged by size: 

                1987    1152    1106     969     789     684     600     564
                 457     456     435     171     135     135      32
 
DraIII CAC_nnn'GTG

Cuts at:      0    7390    9672 
   Size:       7390    2282
 
EaeI y'GGCC_r

Cuts at:      0    4377    5784    5796    5814    5957    6608    9479    9672 
   Size:       4377    1407      12      18     143     651    2871     193

  Fragments arranged by size: 

                4377    2871    1407     651     193     143      18      12
            
 
EagI C'GGCC_G

Cuts at:      0    6608    9672 
   Size:       6608    3064
 
EarI CTCTTCn'nnn_

Cuts at:      0     330     800    3536    4944    5121    5295    5499    6222 
   Size:        330     470    2736    1408     177     174     204     723

Cuts at:   6222    6514    6902    8003    8156    8916    9672 
   Size:        292     388    1101     153     760     756

  Fragments arranged by size: 

                2736    1408    1101     760     756     723     470     388
                 330     292     204     177     174     153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    9249    9416    9672 
   Size:       9249     167     256

  Fragments arranged by size: 

                9249     256     167
 
EcoICRI GAG'CTC

Cuts at:      0     931    4152    4484    9672 
   Size:        931    3221     332    5188

  Fragments arranged by size: 

                5188    3221     931     332
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     781    1285    1729    2123    2236    2603    3197 
   Size:        664     117     504     444     394     113     367     594

Cuts at:   3197    4559    4748    4925    5102    5438    5846    5942    6304 
   Size:       1362     189     177     177     336     408      96     362

Cuts at:   6304    6416    6530    6789    6851    7145    7460    7748    7889 
   Size:        112     114     259      62     294     315     288     141

Cuts at:   7889    8369    8399    8594    8645    8795    9124    9270    9323 
   Size:        480      30     195      51     150     329     146      53

Cuts at:   9323    9547    9672 
   Size:        224     125

  Fragments arranged by size: 

                1362     664     594     504     480     444     408     394
                 367     362     336     329     315     294     288     259
                 224     195     189     177     177     150     146     141
                 125     117     114     113     112      96      62      53
                  51      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2278    2697    9672 
   Size:       2278     419    6975

  Fragments arranged by size: 

                6975    2278     419
 
EcoRI G'AATT_C

Cuts at:      0     609    1332    2421    2855    3363    3577    6400    9327 
   Size:        609     723    1089     434     508     214    2823    2927

Cuts at:   9327    9672 
   Size:        345

  Fragments arranged by size: 

                2927    2823    1089     723     609     508     434     345
                 214
 
EcoRV GAT'ATC

Cuts at:      0    2563    7752    8727    9378    9672 
   Size:       2563    5189     975     651     294

  Fragments arranged by size: 

                5189    2563     975     651     294
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2251    3068    3892    4361    5846    6088    9672 
   Size:       2251     817     824     469    1485     242    3584

  Fragments arranged by size: 

                3584    2251    1485     824     817     469     242
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     817     849    2802    2834    2946    2978    3604    3636 
   Size:        817      32    1953      32     112      32     626      32

Cuts at:   3636    4402    4434    5654    5686    6543    6575    6750    6782 
   Size:        766      32    1220      32     857      32     175      32

Cuts at:   6782    6871    6903    7335    7367    7864    7896    8245    8277 
   Size:         89      32     432      32     497      32     349      32

Cuts at:   8277    9362    9394    9672 
   Size:       1085      32     278

  Fragments arranged by size: 

                1953    1220    1085     857     817     766     626     497
                 432     349     278     175     112      89      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    5651    6788    8459    9672 
   Size:       5651    1137    1671    1213

  Fragments arranged by size: 

                5651    1671    1213    1137
 
HindIII A'AGCT_T

Cuts at:      0    4361    5543    9672 
   Size:       4361    1182    4129

  Fragments arranged by size: 

                4361    4129    1182
 
KpnI G_GTAC'C

Cuts at:      0    8362    9672 
   Size:       8362    1310
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    4570    7825    7942    7987    8634    8844    9672 
   Size:       4570    3255     117      45     647     210     828

  Fragments arranged by size: 

                4570    3255     828     647     210     117      45
 
MscI TGG'CCA

Cuts at:      0    4379    5786    5816    5959    9481    9672 
   Size:       4379    1407      30     143    3522     191

  Fragments arranged by size: 

                4379    3522    1407     191     143      30
 
MslI CAynn'nnrTG

Cuts at:      0     160     563    2403    2741    3488    5674    6315    6666 
   Size:        160     403    1840     338     747    2186     641     351

Cuts at:   6666    9672 
   Size:       3006

  Fragments arranged by size: 

                3006    2186    1840     747     641     403     351     338
                 160
 
MspA1I CmG'CkG

Cuts at:      0    1760    4079    6114    6429    7924    8038    8632    9672 
   Size:       1760    2319    2035     315    1495     114     594    1040

  Fragments arranged by size: 

                2319    2035    1760    1495    1040     594     315     114
            
 
NcoI C'CATG_G

Cuts at:      0    3402    3483    3938    9476    9672 
   Size:       3402      81     455    5538     196

  Fragments arranged by size: 

                5538    3402     455     196      81
 
NdeI CA'TA_TG

Cuts at:      0     145    9672 
   Size:        145    9527
 
NsiI A_TGCA'T

Cuts at:      0     875    9672 
   Size:        875    8797
 
NspI r_CATG'y

Cuts at:      0     873     877    1071    3802    4463    4617    5590    6733 
   Size:        873       4     194    2731     661     154     973    1143

Cuts at:   6733    7220    9154    9559    9672 
   Size:        487    1934     405     113

  Fragments arranged by size: 

                2731    1934    1143     973     873     661     487     405
                 194     154     113       4
 
PasI CC'CwG_GG

Cuts at:      0    2867    9672 
   Size:       2867    6805
 
PciI A'CATG_T

Cuts at:      0    1067    4613    9150    9672 
   Size:       1067    3546    4537     522

  Fragments arranged by size: 

                4537    3546    1067     522
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2707    3262    3629    3772    5317    9672 
   Size:       2707     555     367     143    1545    4355

  Fragments arranged by size: 

                4355    2707    1545     555     367     143
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4789    4821    4966    4998    5143    5175    9672 
   Size:       4789      32     145      32     145      32    4497

  Fragments arranged by size: 

                4789    4497     145     145      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2278    9672 
   Size:       2278    7394
 
PshAI GACnn'nnGTC

Cuts at:      0      72    9672 
   Size:         72    9600
 
PsiI TTA'TAA

Cuts at:      0     395    9520    9672 
   Size:        395    9125     152

  Fragments arranged by size: 

                9125     395     152
 
PspOMI G'GGCC_C

Cuts at:      0    2697    6655    9672 
   Size:       2697    3958    3017

  Fragments arranged by size: 

                3958    3017    2697
 
PstI C_TGCA'G

Cuts at:      0    2982    3174    4923    5100    9672 
   Size:       2982     192    1749     177    4572

  Fragments arranged by size: 

                4572    2982    1749     192     177
 
PvuII CAG'CTG

Cuts at:      0    4079    8038    8632    9672 
   Size:       4079    3959     594    1040

  Fragments arranged by size: 

                4079    3959    1040     594
 
SacI G_AGCT'C

Cuts at:      0     933    4154    4486    9672 
   Size:        933    3221     332    5186

  Fragments arranged by size: 

                5186    3221     933     332
 
SapI GCTCTTCn'nnn_

Cuts at:      0     330     800    9672 
   Size:        330     470    8872

  Fragments arranged by size: 

                8872     470     330
 
ScaI AGT'ACT

Cuts at:      0    6239    6925    9672 
   Size:       6239     686    2747

  Fragments arranged by size: 

                6239    2747     686
 
SexAI A'CCwGG_T

Cuts at:      0    8927    9672 
   Size:       8927     745
 
SfcI C'TryA_G

Cuts at:      0    2265    2978    2993    3170    3240    3521    4919    5096 
   Size:       2265     713      15     177      70     281    1398     177

Cuts at:   5096    7017    7273    7403    8217    8447    9672 
   Size:       1921     256     130     814     230    1225

  Fragments arranged by size: 

                2265    1921    1398    1225     814     713     281     256
                 230     177     177     130      70      15
 
SmlI C'TyrA_G

Cuts at:      0     631    1080    1404    6899    9672 
   Size:        631     449     324    5495    2773

  Fragments arranged by size: 

                5495    2773     631     449     324
 
SpeI A'CTAG_T

Cuts at:      0    7678    9672 
   Size:       7678    1994
 
SphI G_CATG'C

Cuts at:      0     877    3802    9672 
   Size:        877    2925    5870

  Fragments arranged by size: 

                5870    2925     877
 
StyI C'CwwG_G

Cuts at:      0    1464    3402    3483    3938    8093    8493    9366    9476 
   Size:       1464    1938      81     455    4155     400     873     110

Cuts at:   9476    9672 
   Size:        196

  Fragments arranged by size: 

                4155    1938    1464     873     455     400     196     110
                  81
 
SwaI ATTT'AAAT

Cuts at:      0    4371    5694    9672 
   Size:       4371    1323    3978

  Fragments arranged by size: 

                4371    3978    1323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     805    1061    3447    7352    9672 
   Size:        805     256    2386    3905    2320

  Fragments arranged by size: 

                3905    2386    2320     805     256
 
TatI w'GTAC_w

Cuts at:      0     148     621    1570    1606    1725    5373    6237    6726 
   Size:        148     473     949      36     119    3648     864     489

Cuts at:   6726    6923    9672 
   Size:        197    2749

  Fragments arranged by size: 

                3648    2749     949     864     489     473     197     148
                 119      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     600     632    1507    1539    2934    2966    3624    3656 
   Size:        600      32     875      32    1395      32     658      32

Cuts at:   3656    4429    4461    4621    4653    4810    4842    4987    5019 
   Size:        773      32     160      32     157      32     145      32

Cuts at:   5019    5164    5196    5492    5524    7203    7235    8618    8650 
   Size:        145      32     296      32    1679      32    1383      32

Cuts at:   8650    9672 
   Size:       1022

  Fragments arranged by size: 

                1679    1395    1383    1022     875     773     658     600
                 296     160     157     145     145      32      32      32
                  32      32      32      32      32      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0     414    9672 
   Size:        414    9258
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1699    9672 
   Size:       1699    7973
 
XmnI GAAnn'nnTTC

Cuts at:      0      85     410    1457    1742    5873    6098    6535    8255 
   Size:         85     325    1047     285    4131     225     437    1720

Cuts at:   8255    8711    9672 
   Size:        456     961

  Fragments arranged by size: 

                4131    1720    1047     961     456     437     325     285
                 225      85
 
ZraI GAC'GTC

Cuts at:      0     492    9672 
   Size:        492    9180
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AlfI
    AlwNI     ApaI     ApoI     AseI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
   HincII  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI     PasI     PciI    PflMI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI    PvuII     SacI     SapI     ScaI
    SexAI     SfcI     SmlI     SpeI     SphI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI     AgeI     AhdI     AleI     AloI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BglI     BmtI    BsaAI
    BseYI    BsiWI   BssHII    BssSI   BstEII  BstZ17I     DrdI    EcoNI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     MfeI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PfoI
     PmeI     PmlI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     SspI     StuI  Tth111I     XhoI     XmaI
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