Restriction map for pF1KB6596
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          856  GTCTAC               GT^[AC][GT]AC
AccII         228  CGCG                 CG^CG
Alw44I        762  GTGCAC               G^TGCAC
ApaI         1088  GGGCCC               GGGCC^C
ApaLI         762  GTGCAC               G^TGCAC
AvaI          685  CCCGGG               C^[CT]CG[GA]G
AvaII         465  GGTCC                G^G[AT]CC
BbsI          346  GTCTTC               GAAGAC(2/6)
BfaI         1138  CTAG                 C^TAG
BglI          235  GCCTCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         825  AGATCT               A^GATCT
BpuAI         346  GTCTTC               GAAGAC(2/6)
BsaBI         159  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         959  TCCGGT               [AT]^CCGG[AT]
BsmI          247  GCATTC               GAATGC(1/-1)
BsoBI         685  CCCGGG               C^[CT]CG[GA]G
BspMI         852  GCAGGT               ACCTGC(4/8)
BsrFI         982  GCCGGC               [GA]^CCGG[CT]
BstEII        129  GGTGACC              G^GT[ACGT]ACC
BstPI         129  GGTGACC              G^GT[ACGT]ACC
BstUI         228  CGCG                 CG^CG
Cfr10I        982  GCCGGC               [GA]^CCGG[CT]
DraIII       1163  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          239  CCATGG               C^C[GA][CT]GG
EarI         1158  CTCTTC               CTCTTC(1/4)
EcoNI         380  CCTTTGATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       129  GGTGACC              G^GT[ACGT]ACC
EcoT22I       641  ATGCAT               ATGCA^T
HaeII         979  AGCGCC               [GA]GCGC^[CT]
Ksp632I      1158  CTCTTC               CTCTTC(1/4)
MaeI         1138  CTAG                 C^TAG
MamI          159  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          228  CGCG                 CG^CG
NaeI          982  GCCGGC               GCC^GGC
NcoI          239  CCATGG               C^CATGG
NgoMIV        982  GCCGGC               G^CCGGC
NsiI          641  ATGCAT               ATGCA^T
NspI          951  GCATGC               [GA]CATG^[CT]
PpuMI         464  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         107  CAGCTG               CAG^CTG
SacI          885  GAGCTC               GAGCT^C
SapI         1157  GCTCTTC              GCTCTTC(1/4)
SfaNI         558  GCATC                GCATC(5/9)
SgrAI         981  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          685  CCCGGG               CCC^GGG
SphI          951  GCATGC               GCATG^C
StuI          233  AGGCCT               AGG^CCT
TaqI          545  TCGA                 T^CGA
TfiI          659  GAATC                G^A[AT]TC
Tsp509I        93  AATT                 ^AATT
TthHB8I       545  TCGA                 T^CGA
XmaCI         685  CCCGGG               C^CCGGG
XmaI          685  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI          681  GATCC                GGATC(4/5)
              164  GGATC               
BanII         885  GAGCTC               G[GA]GC[CT]^C
             1088  GGGCCC              
BsaI          514  GAGACC               GGTCTC(1/5)
             1063  GAGACC              
BsmFI         689  GGGAC                GGGAC(10/14)
             1134  GTCCC               
Bsp1407I      896  TGTACA               T^GTACA
             1043  TGTACA              
BsrGI         896  TGTACA               T^GTACA
             1043  TGTACA              
BsrI          353  CCAGT                ACTGG(1/-1)
              674  CCAGT               
BssSI         709  CACGAG               CACGAG(-5/-1)
             1093  CACGAG              
BstF5I        406  CATCC                GGATG(2/0)
              559  CATCC               
BstXI         437  CCACCAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1076  CCACAGTGGTGG        
BstYI         680  AGATCC               [GA]^GATC[CT]
              825  AGATCT              
CfoI          729  GCGC                 GCG^C
              980  GCGC                
DdeI           24  CTCAG                C^T[ACGT]AG
              171  CTTAG               
DraII         464  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1087  GGGGCCC             
EcoO109I      464  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1087  GGGGCCC             
EcoT14I       239  CCATGG               C^C[AT][AT]GG
             1118  CCAAGG              
FokI          406  CATCC                GGATG(9/13)
              559  CATCC               
HhaI          729  GCGC                 GCG^C
              980  GCGC                
HinP1I        729  GCGC                 G^CGC
              980  GCGC                
MaeII           8  ACGT                 A^CGT
              149  ACGT                
MflI          680  AGATCC               [GA]^GATC[CT]
              825  AGATCT              
MspA1I        107  CAGCTG               C[AC]G^C[GT]G
              341  CCGCTG              
MwoI          235  GCCTCCATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              343  GCTGTCTTCGC         
PflMI         773  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              866  CCATCATGTGG         
SspBI         896  TGTACA               T^GTACA
             1043  TGTACA              
StyI          239  CCATGG               C^C[AT][AT]GG
             1118  CCAAGG              
Van91I        773  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              866  CCATCATGTGG         
XhoII         680  AGATCC               [GA]^GATC[CT]
              825  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BalI        
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
DpnII       EaeI        EcoRII      Fnu4HI      HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspI        MvaI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       TseI        Tsp45I      
TspRI       XcmI        
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