Restriction map for pF1KB6596
 (Linear) (Six-base) MAPSORT of: KIBB6596.seq  Check: 7338  from: 1  to: 1221



KIBB6596.orf

 With 182 enzymes: SgfI * 

                        November 7, 2014 16:44  ..

AccI GT'mk_AC

Cuts at:      0     857    1221 
   Size:        857     364
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1179    1221 
   Size:        856     323      42

  Fragments arranged by size: 

                 856     323      42
 
AleI CACnn'nnGTG

Cuts at:      0     442     593     652    1081    1221 
   Size:        442     151      59     429     140

  Fragments arranged by size: 

                 442     429     151     140      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     737    1221 
   Size:        737     484
 
ApaI G_GGCC'C

Cuts at:      0    1092    1221 
   Size:       1092     129
 
ApaLI G'TGCA_C

Cuts at:      0     762    1221 
   Size:        762     459
 
AvaI C'yCGr_G

Cuts at:      0     685    1221 
   Size:        685     536
 
BanII G_rGCy'C

Cuts at:      0     889    1092    1221 
   Size:        889     203     129

  Fragments arranged by size: 

                 889     203     129
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339    1221 
   Size:        339     882
 
BfrBI ATG'CAT

Cuts at:      0     643    1221 
   Size:        643     578
 
BglI GCCn_nnn'nGGC

Cuts at:      0     241    1221 
   Size:        241     980
 
BglII A'GATC_T

Cuts at:      0     825    1221 
   Size:        825     396
 
Bme1580I G_kGCm'C

Cuts at:      0     766    1092    1221 
   Size:        766     326     129

  Fragments arranged by size: 

                 766     326     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     735    1221 
   Size:        735     486
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397    1009    1081    1221 
   Size:        397     612      72     140

  Fragments arranged by size: 

                 612     397     140      72
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     508    1057    1221 
   Size:        508     549     164

  Fragments arranged by size: 

                 549     508     164
 
BsaBI GATnn'nnATC

Cuts at:      0     163    1221 
   Size:        163    1058
 
BsaWI w'CCGG_w

Cuts at:      0     959    1221 
   Size:        959     262
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     395     425    1132    1162    1221 
   Size:        395      30     707      30      59

  Fragments arranged by size: 

                 707     395      59      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     684    1111    1114    1194    1221 
   Size:        684     427       3      80      27

  Fragments arranged by size: 

                 684     427      80      27       3
 
BseYI C'CCAG_C

Cuts at:      0     523    1221 
   Size:        523     698
 
BsiHKAI G_wGCw'C

Cuts at:      0     766     889    1217    1221 
   Size:        766     123     328       4

  Fragments arranged by size: 

                 766     328     123       4
 
BsmI GAATG_Cn'

Cuts at:      0     247    1221 
   Size:        247     974
 
Bsp1286I G_dGCh'C

Cuts at:      0     766     889    1092    1217    1221 
   Size:        766     123     203     125       4

  Fragments arranged by size: 

                 766     203     125     123       4
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     843    1221 
   Size:        843     378
 
BsrFI r'CCGG_y

Cuts at:      0     982    1221 
   Size:        982     239
 
BsrGI T'GTAC_A

Cuts at:      0     896    1043    1221 
   Size:        896     147     178

  Fragments arranged by size: 

                 896     178     147
 
BssSI C'ACGA_G

Cuts at:      0     709    1093    1221 
   Size:        709     384     128

  Fragments arranged by size: 

                 709     384     128
 
BstEII G'GTnAC_C

Cuts at:      0     129    1221 
   Size:        129    1092
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     444    1083    1221 
   Size:        444     639     138

  Fragments arranged by size: 

                 639     444     138
 
BstYI r'GATC_y

Cuts at:      0     680     825    1221 
   Size:        680     145     396

  Fragments arranged by size: 

                 680     396     145
 
BtgI C'CryG_G

Cuts at:      0     239    1221 
   Size:        239     982
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     321    1221 
   Size:        321     900
 
BtsI GCAGTG_nn'

Cuts at:      0    1044    1221 
   Size:       1044     177
 
DraIII CAC_nnn'GTG

Cuts at:      0    1168    1221 
   Size:       1168      53
 
EaeI y'GGCC_r

Cuts at:      0     395     550     863     878    1221 
   Size:        395     155     313      15     343

  Fragments arranged by size: 

                 395     343     313     155      15
 
EarI CTCTTCn'nnn_

Cuts at:      0    1164    1221 
   Size:       1164      57
 
EcoICRI GAG'CTC

Cuts at:      0     887    1221 
   Size:        887     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     856    1009    1081    1179    1221 
   Size:        397     459     153      72      98      42

  Fragments arranged by size: 

                 459     397     153      98      72      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     384    1221 
   Size:        384     837
 
EcoO109I rG'GnC_Cy

Cuts at:      0     465    1088    1221 
   Size:        465     623     133

  Fragments arranged by size: 

                 623     465     133
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     972    1221 
   Size:        972     249
 
HaeII r_GCGC'y

Cuts at:      0     983    1221 
   Size:        983     238
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1132    1164    1221 
   Size:       1132      32      57

  Fragments arranged by size: 

                1132      57      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1175    1221 
   Size:       1175      46
 
MscI TGG'CCA

Cuts at:      0     397     552     865     880    1221 
   Size:        397     155     313      15     341

  Fragments arranged by size: 

                 397     341     313     155      15
 
MslI CAynn'nnrTG

Cuts at:      0     127     442     593     595     652    1081    1221 
   Size:        127     315     151       2      57     429     140

  Fragments arranged by size: 

                 429     315     151     140     127      57       2
 
MspA1I CmG'CkG

Cuts at:      0     109     343    1221 
   Size:        109     234     878

  Fragments arranged by size: 

                 878     234     109
 
NaeI GCC'GGC

Cuts at:      0     984    1221 
   Size:        984     237
 
NcoI C'CATG_G

Cuts at:      0     239    1221 
   Size:        239     982
 
NgoMIV G'CCGG_C

Cuts at:      0     982    1221 
   Size:        982     239
 
NsiI A_TGCA'T

Cuts at:      0     645    1221 
   Size:        645     576
 
NspI r_CATG'y

Cuts at:      0     955    1221 
   Size:        955     266
 
PasI CC'CwG_GG

Cuts at:      0     492    1032    1221 
   Size:        492     540     189

  Fragments arranged by size: 

                 540     492     189
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     779     872    1221 
   Size:        779      93     349

  Fragments arranged by size: 

                 779     349      93
 
PpuMI rG'GwC_Cy

Cuts at:      0     465    1221 
   Size:        465     756
 
PspOMI G'GGCC_C

Cuts at:      0    1088    1221 
   Size:       1088     133
 
PvuII CAG'CTG

Cuts at:      0     109    1221 
   Size:        109    1112
 
SacI G_AGCT'C

Cuts at:      0     889    1221 
   Size:        889     332
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1164    1221 
   Size:       1164      57
 
SgrAI Cr'CCGG_yG

Cuts at:      0     982    1221 
   Size:        982     239
 
SmaI CCC'GGG

Cuts at:      0     687    1221 
   Size:        687     534
 
SphI G_CATG'C

Cuts at:      0     955    1221 
   Size:        955     266
 
StuI AGG'CCT

Cuts at:      0     235    1221 
   Size:        235     986
 
StyI C'CwwG_G

Cuts at:      0     239    1118    1221 
   Size:        239     879     103

  Fragments arranged by size: 

                 879     239     103
 
TatI w'GTAC_w

Cuts at:      0     896    1043    1221 
   Size:        896     147     178

  Fragments arranged by size: 

                 896     178     147
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     372     732     873    1077    1221 
   Size:        372     360     141     204     144

  Fragments arranged by size: 

                 372     360     204     144     141
 
XmaI C'CCGG_G

Cuts at:      0     685    1221 
   Size:        685     536
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI     ApaI    ApaLI     AvaI    BanII
     BbsI    BfrBI     BglI    BglII Bme1580I     BplI     BpmI     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrFI    BsrGI    BssSI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NsiI     NspI     PasI    PflMI    PpuMI
   PspOMI    PvuII     SacI     SapI    SgrAI     SmaI     SphI     StuI
     StyI     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI    BsaHI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EagI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NdeI     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI     SmlI    SnaBI     SpeI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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